RNA-seq analysis of transcriptomes for assessing stress tolerance of S. cerevisiae strain, NCIM3186

Abstract

We have previously sequenced the draft genome of high ethanol producing S. cerevisiae strain, NCIM3186. Towards assessing the stress tolerance by this strain transcriptomes from control and in response to glucose, ethanol and furfural stress were sequenced. Comparative RNA-seq analysis of these transcriptomes identified 573 differentially expressed genes of which thiamine biosynthesis genes under furfural stress, TDH1, heat shock proteins and hexose transporter gene under ethanol stress were observed to be highly differentially expressed. Apart from thiamine biosynthesis genes and TDH1, 2 other proteins of unknown function were highly differentially expressed under glucose stress. Most importantly, TAR1 gene was highly down-regulated under all the stress conditions compared to control. Among 93 fermentome genes, 7 (TPS1, TPS2, SIN3, PTK2, SSQ1, ZAP1, DOA4) out of 9 stuck genes are found to be differentially expressed. Several stress-related genes like PHO4, SOD2, STR3, GRE2, GLR1, MEP1,3, MLH3, SNF1, MSN2, ATG1, GLC7 were differentially expressed.

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“…Thiamine, or vitamin B1, is an essential nutrient indispensable for the normal growth and development of various organisms, including humans. In yeast, THI13 expression is differentially expressed under low pH values, such as under furfural stress (Goud and Ulaganathan 2019). The involvement of thiamine in the maintenance of the redox balance in yeast cells under oxidative stress by shifting energy generation from fermentation to respiration has been reported elsewhere (Wolak et al 2014).…”

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“…In fact, most of the molecular markers showed increasing trends of correlation with FTIR profiles at increasing ethanol concentrations, confirming the primary role of the FTIR analytical system in finely characterizing the physiological status of cells in various conditions, including stress [ 18 , 49 , 50 , 51 , 52 ]. Several studies have shown that the transcription of TEF-1α can be induced by increasing stressing condition [ 53 , 54 ]. In addition, FAS-1 , one of the main actors in yeast long-chain fatty acid metabolism, was interested in stress response to ethanol [ 55 , 56 ], antifoaming compounds [ 57 ] and high temperature [ 58 ], whereas the mitochondrial COX gene is a known to be part of the cellular response to different types of stressing agents, such as ethanol or high temperatures [ 59 ].…”