RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri

Liao, Jinxing; Li, Kaihuai; Wang, Jinpei; Deng, Jia-Ru; Liu, Qiongguang; Chang, Changqing

doi:10.1186/s12864-019-6193-0

Cited by 14 publications

(4 citation statements)

References 63 publications

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“…Typically, the proportion of unsaturated and short‐chained FAs increases with decreasing growth temperature [35–37]. For example in Xanthomonas citri , membrane adaptation to cold is mediated by the specific increase in cis ‐C16:1 FA in parallel with an increase in anteiso‐C15:0 relative to iso‐C15:0 [38]. A similar pattern was discernible in X. campestris (Fig.…”

Section: Discussionmentioning

confidence: 83%

Characterization of multiple lysophosphatidic acid acyltransferases in the plant pathogen Xanthomonas campestris

Vasilopoulos,

Heflik,

Czolkoss

et al. 2023

The FEBS Journal

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Phosphatidic acid (PA) is the precursor of most phospholipids like phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. In bacteria, its biosynthesis begins with the acylation of glycerol‐3‐phosphate to lysophosphatidic acid (LPA), which is further acylated to PA by the PlsC enzyme. Some bacteria, like the plant pathogen Xanthomonas campestris, use a similar pathway to acylate lysophosphatidylcholine to phosphatidylcholine (PC). Previous studies assigned two acyltransferases to PC formation. Here, we set out to study their activity and found a second much more prominent function of these enzymes in LPA to PA conversion. This PlsC‐like activity was supported by the functional complementation of a temperature‐sensitive plsC‐deficient Escherichia coli strain. Biocomputational analysis revealed two further PlsC homologs in X. campestris. The cellular levels of the four PlsC‐like proteins varied with respect to growth phase and growth temperature. To address the question whether these enzymes have redundant or specific functions, we purified two recombinant, detergent‐solubilized enzymes in their active form, which enabled the first direct biochemical comparison of PlsC isoenzymes from the same organism. Overlapping but not identical acyl acceptor and acyl donor preferences suggest redundant and specialized functions of the X. campestris PlsC enzymes. The altered fatty acid composition in plsC mutant strains further supports the functional differentiation of these enzymes.

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Section: Discussionmentioning

confidence: 83%

Characterization of multiple lysophosphatidic acid acyltransferases in the plant pathogen Xanthomonas campestris

Vasilopoulos,

Heflik,

Czolkoss

et al. 2023

The FEBS Journal

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“…Differentially Expressed Genes (DEGs) were identified in the sshog1Δ mutant compared to the wild‐type MAT‐1 strain. We filtered DEGs of significance ( p ≤ 0.05) as described (Yan et al ., 2016a; Liao et al ., 2019). A total of 1100 DEGs were listed in supplementary Table S2.…”

Section: Resultsmentioning

confidence: 99%

MAP kinase Hog1 mediates a cytochrome P450 oxidoreductase to promote the Sporisorium scitamineum cell survival under oxidative stress

Cai

Deng

et al. 2021

Environmental Microbiology

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The MAP kinase high osmolarity glycerol 1 (Hog1) plays a central role in responding to external oxidative stress in budding yeast Saccchromyces cerevisiae. However, the downstream responsive elements regulated by Hog1 remain poorly understood. In this study, we report that a Sporisorium scitamineum orthologue of Hog1, named as SsHog1, induced transcriptional expression of a putative cytochrome P450 oxidoreductase encoding gene SsCPR1, to antagonize oxidative stress. We found that upon exposure to hydrogen peroxide (H 2 O 2 ), SsHog1 underwent strikingly phosphorylation, which was proved to be critical for transcriptional induction of SsCPR1. Loss of SsCPR1 led to hypersensitive to oxidative stress similar as the sshog1Δ mutant did, but was resistant to osmotic stress, which is different from the sshog1Δ mutant. On the other hand, overexpression of SsCPR1 in the sshog1Δ mutant could partially restore its ability of oxidative stress tolerance, which indicated that the Hog1 MAP kinase regulates the oxidative stress response specifically through cytochrome P450 (SsCpr1) pathway. Overall, our findings highlight a novel MAPK signalling pathway mediated by Hog1 in regulation of the oxidative stress response via the cytochrome P450 system, which plays an important role in host-fungus interaction.

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“…Meanwhile, transcriptome sequencing has been widely used to study the growth and development patterns, stress physiology, stress response mechanisms, and metabolic regulatory networks of plants [53][54][55][56]. In this study, transcriptome sequencing was conducted on the wild-type plants and miR2871b overexpression plants to obtain a more comprehensive understanding of the regulation of miR2871b.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA2871b of Dongxiang Wild Rice (Oryza rufipogon Griff.) Negatively Regulates Cold and Salt Stress Tolerance in Transgenic Rice Plants

Yang,

Chen,

Gao

et al. 2023

IJMS

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Cold and salt stresses are major environmental factors that constrain rice production. Understanding their mechanisms is important to enhance cold and salt stress tolerance in rice. MicroRNAs (miRNAs) are a class of non-coding RNAs with only 21–24 nucleotides that are gene regulators in plants and animals. Previously, miR2871b expression was suppressed by cold stress in Dongxiang wild rice (DXWR, Oryza rufipogon Griff.). However, its biological functions in abiotic stress responses remain elusive. In the present study, miR2871b of DWXR was overexpressed to investigate its function under stress conditions. When miR2871b of DWXR was introduced into rice plants, the transgenic lines were more sensitive to cold and salt stresses, and their tolerance to cold and salt stress decreased. The increased expression of miR2871b in rice plants also increased the levels of reactive oxygen species (ROS) and malondialdehyde (MDA); however, it markedly decreased the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) and the contents of proline (Pro) and soluble sugar (SS). These data suggested that miR2871b of DXWR has negative regulatory effects on cold and salt stress tolerance. Meanwhile, 412 differentially expressed genes (DEGs) were found in rice transgenic plants using transcriptome sequencing, among which 266 genes were up-regulated and 146 genes were down-regulated. Furthermore, the upstream cis-acting elements and downstream targets of miR2871b were predicted and analyzed, and several critical acting elements (ABRE and TC-rich repeats) and potential target genes (LOC_Os03g41200, LOC_Os07g47620, and LOC_Os04g30260) were obtained. Collectively, these results generated herein further elucidate the vital roles of miR2871b in regulating cold and salt responses of DXWR.

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RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri

Cited by 14 publications

References 63 publications

Characterization of multiple lysophosphatidic acid acyltransferases in the plant pathogen Xanthomonas campestris

Characterization of multiple lysophosphatidic acid acyltransferases in the plant pathogen Xanthomonas campestris

MAP kinase Hog1 mediates a cytochrome P450 oxidoreductase to promote the Sporisorium scitamineum cell survival under oxidative stress

MicroRNA2871b of Dongxiang Wild Rice (Oryza rufipogon Griff.) Negatively Regulates Cold and Salt Stress Tolerance in Transgenic Rice Plants

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