RNA-seq reveals temporal differences in the transcriptome response to acute heat stress in the Atlantic salmon (Salmo salar)

SMRT sequencing of the full-length transcriptome of Odontotermes formosanus (Shiraki) under Serratia marcescens treatment

“…A total of 1 μg RNA per sample was used as input material for RNA sample preparation. Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute the sequences to each sample 24 – 26 . In short, mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads.…”

“…A total of 69 unigenes were divided into 26 functional groups. For the BP category, the dominant subcategories were metabolic process (35) and cellular process (29), followed by single-organism process (26). In the CC category, cell part (28), cell (28) and organelle (21) were highly enriched.…”

The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia

“…In addition, HSPs have a wider role in relation to the function of the immune system (i.e., antigen presentation) [84,85], apoptosis [86] and protection from oxidative stress [87]. HSPs are essential regulators of the cellular stress response in aquatic ectotherms [88], and their expression is well characterized in teleost species as they function as protective proteins against acute and chronic thermal stress [13][14][15][16][17][21][22][23] and during hypoxia [26,29,30]. After the incremental temperature challenge to 20 °C (alone or combined with hypoxia), we found enriched pathways related to the heat shock response, protein folding and protein stability (Theme # 1) (i.e., 'chaperone-mediated autophagy processes', 'chaperone and protein folding responses', 'STIP1(HOP) binds HSP90 and HSP70:HSP40:nascent protein').…”

“…Thermal stress responses in sh have been widely investigated, and involve the expression of evolutionarily conserved stress-responsive genes [12]. A number of studies have demonstrated that acute and/or long-term exposure to high temperatures results in extensive changes in gene transcription in different salmonid tissues [13][14][15][16][17][18][19][20]. These molecular responses include alterations in the expression of genes related to the heat shock response, protein folding and repair, stress-induced cell death/apoptosis, signal transduction, oxidative stress, the in ammatory response, and a diversity of metabolic processes [13][14][15][16][17][20][21][22][23].…”

“…The liver was chosen to study due to its roles in a number of biological processes including the stress response, nutrient metabolism and immunity [24,43], and that it has previously been shown to be an excellent target organ to characterize temperature and hypoxia stress response mechanisms in this sh species [14,16]. An Agilent 44K salmonid oligonucleotide microarray platform [44] was employed in the current study to initially assess hepatic transcriptome changes and to elucidate the processes and mechanisms involved in the liver's response once temperature reached 20 °C.…”