RNA sequencing for global gene expression associated with muscle growth in a single male modern broiler line compared to a foundational Barred Plymouth Rock chicken line

Kong, Byung-Whi; Hudson, Nicholas J.; Seo, Dongwon; Lee, Seok; Khatri, Bhuwan; Lassiter, Kentu; Cook, Devin; Piekarski, Alissa; Dridi, Sami; Anthony, Nicholas B.; Bottje, Walter

doi:10.1186/s12864-016-3471-y

Cited by 63 publications

(62 citation statements)

References 93 publications

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“…Furthermore, interest in this transcription factor goes beyond stress. For example, when a comparison of a single male modern broiler line compared to a foundational Barred Plymouth Rock chicken line was conducted, it showed that Nrf2 expression in breast muscle was much higher in the modern broiler compared to the foundational BPR line [299], and it was suggested Nrf2-mediated oxidative stress response pathways are involved in breast muscle growth in chickens [300]. In recent years, several compounds, including carnitine, have been shown to have inhibitory activities against multiple components of the NF-κB activation pathway.…”

Section: Other Nutrients and Probioticsmentioning

confidence: 99%

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

et al. 2019

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Poultry in commercial settings are exposed to a range of stressors. A growing body of information clearly indicates that excess ROS/RNS production and oxidative stress are major detrimental consequences of the most common commercial stressors in poultry production. During evolution, antioxidant defence systems were developed in poultry to survive in an oxygenated atmosphere. They include a complex network of internally synthesised (e.g., antioxidant enzymes, (glutathione) GSH, (coenzyme Q) CoQ) and externally supplied (vitamin E, carotenoids, etc.) antioxidants. In fact, all antioxidants in the body work cooperatively as a team to maintain optimal redox balance in the cell/body. This balance is a key element in providing the necessary conditions for cell signalling, a vital process for regulation of the expression of various genes, stress adaptation and homeostasis maintenance in the body. Since ROS/RNS are considered to be important signalling molecules, their concentration is strictly regulated by the antioxidant defence network in conjunction with various transcription factors and vitagenes. In fact, activation of vitagenes via such transcription factors as Nrf2 leads to an additional synthesis of an array of protective molecules which can deal with increased ROS/RNS production. Therefore, it is a challenging task to develop a system of optimal antioxidant supplementation to help growing/productive birds maintain effective antioxidant defences and redox balance in the body. On the one hand, antioxidants, such as vitamin E, or minerals (e.g., Se, Mn, Cu and Zn) are a compulsory part of the commercial pre-mixes for poultry, and, in most cases, are adequate to meet the physiological requirements in these elements. On the other hand, due to the aforementioned commercially relevant stressors, there is a need for additional support for the antioxidant system in poultry. This new direction in improving antioxidant defences for poultry in stress conditions is related to an opportunity to activate a range of vitagenes (via Nrf2-related mechanisms: superoxide dismutase, SOD; heme oxygenase-1, HO-1; GSH and thioredoxin, or other mechanisms: Heat shock protein (HSP)/heat shock factor (HSP), sirtuins, etc.) to maximise internal AO protection and redox balance maintenance. Therefore, the development of vitagene-regulating nutritional supplements is on the agenda of many commercial companies worldwide.

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Section: Other Nutrients and Probioticsmentioning

confidence: 99%

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

et al. 2019

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“…Oxidative stress and impaired glycolysis can both arise due to mitochondrial dysfunction. Multiple studies have confirmed the transcriptomic [9,18] and metabolomic signatures of oxidative stress in WB [10], and have also suggested mitochondrial dysfunction [43]. Furthermore, genes essential to glycolysis, angiogenesis, and apoptosis (up-regulated in WB) are transcriptionally regulated in hypoxic conditions [44,45], that are typical of tissue under oxidative stress.…”

Section: Discussionmentioning

confidence: 87%

Insights into the molecular basis of wooden breast based on comparative analysis of fast- and slow-growth broilers

Hubert

Williams

Athrey

2018

Preprint

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Wooden breast has emerged as an important condition in the poultry industry and affects the breast muscle of commercial meat-type chickens (broilers). Thus far, the condition has been classified as a myopathy, confirmed to not have an infectious origin, with molecular data showing the muscle to be under oxidative stress. The objective in this study was to query the functional origins of wooden breast and reveal its molecular similarities to known conditions based on pathway analyses. To carry out an in-depth comparative analysis, we generated RNAseq data from wooden breast affected birds and incorporated breast specific transcriptomic data from previously published studies. The comparative datasets were constructed from a range of commercial fast-growth and slow-growth varieties. Analysis of high-impact variants identified from transcriptome data provided a list of genes important in cell signaling, cell proliferation, cytoskeletal development, and calcium metabolism as being affected by nucleotide changes.Overlaying the lists of significantly differentially expressed genes with the list of high-impact variants produced a list of twenty genes that suggest an association of mechanistic and functional causes for woody breast; supporting a polygenic basis for this condition. Our study demonstrates that wooden breast shows an age-dependent gene expression pattern, with pathway analysis showing enrichment of glycolysis, cell differentiation, tumor suppression and inhibition, further indicating a complex condition with few similarities to myopathies. In summary, our results indicate the existence of a mechanistic, heritable basis for wooden breast, the drivers of which deserve more in-depth investigation. Additionally, they also suggest wooden breast to be a more complex condition than previously reported, potentially involving other organ systems.3

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“…33 Kong et al reported that upregulation of LMOD2 gene responsible for skeletal muscle growth in broiler chickens. 34 Phosphoglycerate dehydrogenase (PHGDH) is a key metabolic enzyme in de novo serine biosynthesis. 35 Brown et al suggested that increased expression of PHGDH genes enhanced the skeletal muscle growth in ractopamine treated pigs.…”

Section: Discussionmentioning

confidence: 99%

Modulatory effect of Kolin PlusTM, a polyherbal formulation on muscle growth development in choline deficient diet model of broilers: a microarray approach

et al. 2019

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Background: The present study was conducted to elucidate the genes and its associated pathways delineating the growth-promoting potential of polyherbal formulation (PHF), Kolin PlusTM using microarray in Cobb 430 broiler chickens.Methods: Microarray was performed for four groups, namely, normal diet (ND) as G1, choline chloride deficient (CCD) diet as G2, choline chloride (CCL, 400 g/ton) as G3 and PHF (400 g/ton) as G4. Breast muscle samples were collected, and the growth-related gene expression profile was measured using the Agilent microarray platform. Results: Totally 2900 differentially expressed genes (DEGs) in muscle tissue sample was revealed using hierarchical clustering based on the similarity of their expression profiles, which further allows the user to pick out groups of similar genes. Among them, 1000, 364 and 481 genes were significantly upregulated and 244, 485 and 326 genes were significantly downregulated between ND and CCD, CCL and CCD, PHF and CCD respectively. Furthermore, some of the focused genes (CSRP3, SOX10, BCO1, CALB1, LMOD2, KLF15, CTHRC1, PHGDH, UTS2R, and ANKRD2) were significantly (p<0.05) modulated by PHF (400 g/ton) supplementation in birds fed with CCD diet. These genes play an essential role in protein translation, energy metabolism, and muscle growth promotion.Conclusions:It may be concluded that supplementation of PHF at 400 g/ton of feed could positively influence the certain focused genes associated with muscle growth promotion, which favours the productive phenotypic response in broiler chickens fed with CCD diet.

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RNA sequencing for global gene expression associated with muscle growth in a single male modern broiler line compared to a foundational Barred Plymouth Rock chicken line

Cited by 63 publications

References 93 publications

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

Insights into the molecular basis of wooden breast based on comparative analysis of fast- and slow-growth broilers

Modulatory effect of Kolin PlusTM, a polyherbal formulation on muscle growth development in choline deficient diet model of broilers: a microarray approach

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