RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells

Rodd, Annabelle L; Ververis, Katherine; Sayakkarage, Dheeshana; Khan, Abdul Waheed; Rafehi, Haloom; Ziemann, Mark; Loveridge, Shanon J.; Lazarus, Ross; Kerr, Caroline A; Lockett, Trevor; El‐Osta, Assam; Karagiannis, Tom C.; Bennett, Louise

doi:10.1039/c5fo00467e

Cited by 8 publications

(6 citation statements)

References 93 publications

(107 reference statements)

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“…26 Similarly, other bioactive compounds from the plants have the ability to arrest the cell cycle at S and G2/M phase in different cancer cell lines. 5,31,35,36 3.6. Effect of MH on cyclin, CDK, p21 waf1/Cip1 and p27 kip1 mRNA expression in HCT-116 and…”

Section: Mh Induces Cell Cycle Arrest Of Hct 116 and Lovo Cellsmentioning

confidence: 99%

The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle

et al. 2018

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Numerous investigations have been made on plant phenolic compounds and cancer prevention in recent decades. Manuka honey (MH) represents a good source of phenolic compounds such as luteolin, kaempferol, quercetin, gallic acid and syringic acid. The aim of this work was to evaluate the chemopreventive effects of MH on human colon cancer HCT-116 and LoVo cells. Both cells were exposed to different concentrations of MH (0-20 mg mL-1 for HCT-116 cells and 0-50 mg mL-1 for LoVo cells) for 48 h to measure apoptosis and cell cycle arrest as well as apoptosis and cell cycle regulatory gene and protein expression. MH exhibited profound inhibitory effects on cellular growth by reducing the proliferation ability, inducing apoptosis and arresting the cell cycle in a dose-dependent manner. Interestingly, MH treatment in non-malignant cells did not exert any significant toxicity at similar concentrations. The apoptosis event was associated with the increasing expression of p53, cleaved-PARP and caspase-3 and with the activation of both intrinsic (caspase-9) and extrinsic (caspase-8) apoptotic pathways. MH induced cell cycle arrest in the S phase in HCT-116 cells, and simultaneously, in LoVo cells, it occurred in the G2/M phase through the modulation of cell cycle regulator genes (cyclin D1, cyclin E, CDK2, CDK4, p21, p27 and Rb). The expression of p-Akt was suppressed while the expression of p-p38MAPK, p-Erk1/2 and endoplasmic stress markers (ATF6 and XBP1) was increased for apoptosis induction. Overall, these findings indicate that MH could be a promising preventive or curative food therapy for colon cancer.

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Section: Mh Induces Cell Cycle Arrest Of Hct 116 and Lovo Cellsmentioning

confidence: 99%

The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle

et al. 2018

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“…Anticancer: Polar and apolar extracts and essential oil of laurel fruits and leaves present important anticancer activities for various types of cancer, such as leukemia [45,50,52,94,191,[202][203][204], fibrosarcoma [195], neuroblastoma [205][206][207], liver [27], breast [14,27,208], ovarian [39], prostatic [209], colon [27,96,210], colorectal [96], renal [209], skin [211], cervical [27,212], lung [27,213], and intestinal cancer [50].…”

Section: Review On Laurus Nobilismentioning

confidence: 99%

Phytochemicals and Biological Activities of Laurel Tree (Laurus nobilis)

Alejo-Armijo

Altarejos

Salido

2017

Natural Product Communications

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This review summarizes the chemical composition reported up to date on Laurus nobilis L. (Lauraceae), an evergreen shrub or tree cultivated for its aromatic leaves and ornamental interest. It has been focused on non-volatile phytochemicals such as sesquiterpene lactones, flavonoids and proanthocyanidins, among others. Moreover, biological activities of laurel extracts and pure compounds have also been reviewed.

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“…These findings are very similar to those of the present study. There is relatively little known about the effect of bay leaf on the cell cycle and apoptosis in CRC cells with the exception of, to the authors’ knowledge, the work of Rodd et al [ 78 ], who reported that unfractionated and low molecular mass fractions of bay leaf were pro-apoptotic (based on caspase-3/7 activity) although their impact varied. In addition, they halted the cell cycle via arrest in the G1/S phase.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding p53, the slight reduction in pro-apoptotic p53 caused by TE was unexpected. However, the HCA-7 cell line has a partially mutated/dysfunctional p53, which in fact can suppress apoptosis [ 78 , 79 ]. Thus, the most likely explanation therefore is that TE simply reduced mutated p53.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Culinary Herbs and Spices on the Growth of HCA-7 Colorectal Cancer Cells and Their COX-2 Expression

et al. 2017

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It is unclear if the anti-inflammatory properties of culinary herbs and spices (CHS) are linked to their ability to inhibit Colorectal cancer cell (CRC) growth. Furthermore, their therapeutic potential with regards to CRC is unknown. The aim of this study was to establish if the inhibition of HCA-7 CRC cell growth by a selection of culinary herbs and spices (CHS) is linked to the inhibition of the cells’ cyclooxygenase-2 (COX-2 )expression, and to investigate their therapeutic potential. CHS inhibited the growth of Human colon adenocarcinoma-7 (HCA-7) cells; the order of potency was turmeric, bay leaf, ginger, sage, and rosemary; their combinations had a synergistic or additive effect on cell growth inhibition. CHS also inhibited COX-2 expression and activity; this action was comparable to that of the specific COX-2 inhibitor Celecoxib. Coincident with COX-2 inhibition was the accumulation of cells in the sub G1 phase of the HCA-7’s cell cycle and, using bay leaf and turmeric, the cleavage of caspase 3 and poly (ADP-ribose) polymerase (PARP). This latter effect showed that the effect of these CHS on growth arrest was irreversible, and was comparable to that of the caspase activator Etoposide. This study provides evidence of a link between the inhibition of HCA-7 growth, and its COX-2 expression, by CHS, and their therapeutic potential.

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RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells

Abstract: DNA double strand breaks mediated by high mass bay leaf fraction in HT-29 cells.

Cited by 8 publications

References 93 publications

The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle

The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle

Phytochemicals and Biological Activities of Laurel Tree (Laurus nobilis)

Inhibitory Effects of Culinary Herbs and Spices on the Growth of HCA-7 Colorectal Cancer Cells and Their COX-2 Expression

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