RNA transcript expression of IGF-I/PI3K pathway components in regenerating skeletal muscle is sensitive to initial injury intensity

Matheny, Ronald W.; Carrigan, Christopher T.; Abdalla, Mary N.; Geddis, Alyssa V.; Leandry, Luis A.; Aguilar, Carlos A.; Hobbs, Stuart S.; Urso, Maria L.

doi:10.1016/j.ghir.2016.09.002

Cited by 20 publications

(11 citation statements)

References 54 publications

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“…miR-206 is a muscle-specific miRNA and its increase in postprandial lipemia suggests it is released from this tissue in response to a fat challenge, although no significant changes were observed in its tissue of origin (Figure 4B). Several other studies have reported their secretion upon muscle damage [55]. miR-409-3p is a brain-enriched miRNA with very high expression in the pituitary gland, but it is also expressed in other brain regions.…”

Section: Resultsmentioning

confidence: 99%

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

et al. 2019

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Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are poorly described. We aimed to profile circulating miRNAs in response to a fat challenge. In total, 641 circulating miRNAs were assessed by real-time PCR in plasmas from mice two hours after lipid gavage. Mice with intestine-specific loss of Dicer were screened to identify potential miRNAs released by the intestine. A total of 68 miRNAs were selected for further validation. Ten circulating miRNAs were finally validated as responsive to postprandial lipemia, including miR-206-3p, miR-543-3p, miR-466c-5p, miR-27b-5p, miR-409-3p, miR-340-3p, miR-1941-3p, miR-10a-3p, miR-125a-3p, and miR-468-3p. Analysis of their possible tissues of origin/target showed an enrichment of selected miRNAs in liver, intestine, brain, or skeletal muscle. miR-206, miR-27b-5p, and miR-409-3p were validated in healthy humans. Analysis of their predicted target genes revealed their potential involvement in insulin/insulin like growth factor (insulin/IGF), angiogenesis, cholecystokinin B receptor signaling pathway (CCKR), inflammation or Wnt pathways for mice, and in platelet derived growth factor (PDGF) and CCKR signaling pathways for humans. Therefore, the current study shows that certain miRNAs are released in the circulation in response to fatty meals, proposing them as potential novel therapeutic targets of lipid metabolism.

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Section: Resultsmentioning

confidence: 99%

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

et al. 2019

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“…In this combined regulatory network, gene PIK3CB, VEGFA, MAPK14, PIK3R1, and CASP3 were identified as hub genes, and their related miRNAs were detected as hub miRNAs. As a potential related miRNA of PIK3CB, miR-206 was reported as a regulatory miRNA on cell proliferation and apoptosis [ 50 , 51 ], and was identified with PIK3CB showing sensitive to initial injury intensity induced by freeze damage [ 52 ]. Another potential related miRNA of PIK3CB, miR-125a, was reported as important regulation miRNA on metabolism [ 53 ] and cell development [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of microRNAs in the intestinal tissues of sheep (Ovis aries)

Hou

Wang

et al. 2018

PLoS ONE

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Sheep are small ruminants, and their long intestines exhibit high digestive and absorptive capacity in many different rearing conditions; however, the genetic bases of this characteristic remains unclear. MicroRNAs (miRNAs) play a major role in maintaining both intestinal morphological structure as well as in regulating the physiological functions of this organ. However, no study has reported on the miRNA expression profile in the intestinal tissues of sheep. Here, we analyzed and identified the miRNA expression profile of three different intestinal tissues (i.e., duodenum, cecum, and colon) of sheep (Ovis aries) using high-throughput sequencing and bioinformatic methods. In total, 106 known miRNAs were identified, 458 conserved miRNAs were detected, 192 unannotated novel miRNAs were predicted, and 195 differentially expressed miRNAs were found between the different tissues. Additionally, 3,437 candidate target genes were predicted, and 17 non-redundant significantly enriched GO terms were identified using enrichment analysis. A total of 99 candidate target genes were found to significantly enriched in 4 KEGG biological pathways. A combined regulatory network was constructed based on 92 metabolism-related candidate target genes and 65 differentially expressed miRNAs, among which 7 miRNAs were identified as hub miRNAs. Via these mechanisms, miRNAs may play a role in maintaining intestinal homeostasis and metabolism. This study helps to further explain the mechanisms that underlie differences in tissue morphology and function in three intestinal segments of sheep.

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“…Among all mature miRNAs, only miR-1 is up-regulated, while there is no change for the expression of miR-133a and miR-206 after taking exercise for 3 and 6 h [ 86 ]. Through IGF-1/Akt signal pathway, the expression of miR-1 can promote protein synthesis and hypertrophy of skeletal muscle [ 87 ]. After taking 12-week resistance exercise, 56 adult subjects are divided into high-response team and low-response team based on their changes of lean body weight (LBW).…”

Section: Effect Of Exercise On Mirnamentioning

confidence: 99%

Regulatory Role of MicroRNAs in Muscle Atrophy during Exercise Intervention

Zhang

Chen

2018

IJMS

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Skeletal muscle comprising approximately 40% of body weight is highly important for locomotion and metabolic homeostasis. The growth and regeneration of skeletal muscle are highly organized processes; thus, it is not surprising to reveal certain complexity during these regulatory processes. Recently, a large number of evidence indicate that microRNAs can result in obvious impacts on growth, regeneration and metabolism of skeletal muscle. In this review, recent research achievements of microRNAs in regulating myogenesis, atrophy and aging during exercise intervention are discussed, which will provide the guidance for developing potential applications of microRNAs in health promotion and rehabilitation of sports injuries.

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RNA transcript expression of IGF-I/PI3K pathway components in regenerating skeletal muscle is sensitive to initial injury intensity

Abstract: During early skeletal muscle regeneration in mice, transcript expressions for some components of the IGF-I/PI3K pathway are sensitive to initial injury intensity induced by freeze damage.

Cited by 20 publications

References 54 publications

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

Identification and characterization of microRNAs in the intestinal tissues of sheep (Ovis aries)

Regulatory Role of MicroRNAs in Muscle Atrophy during Exercise Intervention

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