RNAi mediated IL-6 in vitro knockdown in psoriasis skin model with topical siRNA delivery system based on liquid crystalline phase

Depieri, Lívia Vieira; Borgheti-Cardoso, Lívia Neves; Campos, Patrícia Mazureki; Otaguiri, Katia Kaori; Vicentini, Fabiana T. M. C.; Lopes, Luciana B.; Fonseca, Maria José Vieira; Bentley, Maria Vitória Lopes Badra

doi:10.1016/j.ejpb.2016.05.012

Cited by 52 publications

(35 citation statements)

References 52 publications

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“…[4][5][6][7][8] Up to date, topical therapy remains a preferred treatment strategy for psoriasis because the site of action is direct. The challenge for topical treatment of psoriasis is the poor drug penetration through the psoriatic skin owing to the physiopathology of psoriasis.…”

Section: Introductionmentioning

confidence: 99%

Effects of nanoparticles with hydrotropic nicotinamide on tacrolimus: permeability through psoriatic skin and antipsoriatic and antiproliferative activities

Wan

Pan

Long

et al. 2017

IJN

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The hybrid system based on nanoparticles (NPs) self-assembled by the conjugations of hyaluronic acid with cholesterol (HA–Chol NPs) combined with nicotinamide (NIC) for tacrolimus (FK506), ie, FK506 NPs–NIC, has been confirmed to exhibit a significant synergistic effect on FK506 permeation through and into intact skin; thus, it may be a promising approach for FK506 to effectively treat skin diseases. The aim of this study was to evaluate its potential for the treatment of psoriasis. In vitro permeation through the psoriatic skin was carried out, and the results revealed that the combination of NPs with NIC exhibited a significant synergistic effect on FK506 deposition within the psoriatic skin (3.40±0.67 μg/cm 2 ) and penetration through the psoriatic skin (30.86±9.66 μg/cm 2 ). The antipsoriatic activity of FK506 NPs–NIC was evaluated through the treatment for imiquimod (IMQ)-induced psoriasis. The psoriasis area and severity index (PASI) score demonstrated that FK506 HA–Chol NPs–NIC exerted the effect on ameliorating the skin lesions comparable to clobetasol propionate (a positive drug for psoriasis) and superior to commercial FK506 ointment (Protopic ® ), and the histological study showed that it presented a synergistic effect on antipsoriasis after FK506 incorporation into NPs combined with NIC hydrotropic system, which might ultimately increase the therapeutic effect and minimize the systemic side effects by reducing the overall dose of FK506. RAW 264.7 cell uptake presented the enhancement of drugs delivered into cells by HA–Chol NPs–NIC. The antiproliferative activity on HaCaT cells identified that FK506 HA–Chol NPs–NIC exhibited significant inhibiting effects on HaCaT proliferation. The results support that the combination of HA–Chol NPs with NIC is a promising approach for FK506 for the treatment of psoriasis.

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Section: Introductionmentioning

confidence: 99%

Effects of nanoparticles with hydrotropic nicotinamide on tacrolimus: permeability through psoriatic skin and antipsoriatic and antiproliferative activities

Wan

Pan

Long

et al. 2017

IJN

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“…This can be achieved through a carrier matrix that remains at the site of administration and releases therapeutic substances in a controlled manner. Examples of systems for local administration are injectable in situ gelling materials [49][50][51], beads [48], inhalable particles [36,52,53], nanocarriers that remain at the site of administrations [54][55][56], microneedles [57,58], liquid crystalline phases [59], creams and ointments [60,61] and metal drug releasing implants [62,63].…”

Section: Drug Delivery Systems For Local Administration: Basic Conceptsmentioning

confidence: 99%

“…The extended therapeutic duration also puts demand on protection of sensitive therapeutic molecules that are prone to degradation in the body, such as RNA. Again, nanoparticles and nanostructured materials can be used to protect the substances until released to their targets, as demonstrated for RNA [56,59,69,70]. This protective effect can be achieved by denying enzymes, such as RNAase, access to the therapeutic molecules, by providing a suitable chemical environment, and by keeping the substances in a stable physicochemical state.…”

Section: Drug Delivery Systems For Local Administration: Basic Conceptsmentioning

confidence: 99%

Local co-administration of gene-silencing RNA and drugs in cancer therapy: State-of-the art and therapeutic potential

Larsson

Huang

LIu

et al. 2017

Cancer Treatment Reviews

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Gene-silencing miRNA and siRNA are emerging as attractive therapeutics with potential to suppress any genes, which could be especially useful in combination cancer therapy to overcome multidrug resistant (MDR) cancer. Nanomedicine aims to advance cancer treatment through functional nanocarriers that delivers one or more therapeutics to cancer tissue and cells with minimal off-target effects and suitable release kinetics and dosages. Although much effort has gone into developing circulating nanocarriers with targeting functionality for systemic administration, another alternative and straightforward approach is to utilize formulations to be administered directly to the site of action, such as pulmonary and intratumoral delivery. The combination of gene-silencing RNA with drugs in nanocarriers for localized delivery is emerging with promising results. In this review, the current progress and strategies for local co-administration of RNA and drug for synergistic effects and future potential in cancer treatment are presented and discussed. Key advances in RNA-drug anticancer synergy and localized delivery systems were combined with a review of the available literature on local co-administration of RNA and drug for cancer treatment. It is concluded that advanced delivery systems for local administration of gene-silencing RNA and drug hold potential in treatment of cancer, depending on indication. In particular, there are promising developments using pulmonary delivery and intratumoral delivery in murine models, but further research should be conducted on other local administration strategies, designs that achieve effective intracellular delivery and maximize synergy and feasibility for clinical use.

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“…Entretanto, em ensaios pré clínicos e pesquisas científicas, outras nanopartículas de base lipídicas estão sendo estudadas para tratar não somente o câncer de pele, mas outras doenças de pele través do uso de siRNAs, tais como vitiligo, psoríase, acne, feridas, dermatites, inflamações cutâneas, entre outras (BORGHETI-CARDOSO et al, 2017;DEPIERI et al, 2016;PETRILLI et al, 2016;ROSA et al, 2018;TOFANI et al, 2018). (XU, XIAOYANG et al, 2015).…”

Section: Nanopartículas Lipídicas Como Vetores De Sirnaunclassified

“…siRNAs vem sendo aplicados para tratar doenças, porém, o grande desafio para estas moléculas são as barreiras biológicas, que no caso da pele, é o EC. Para superar estas dificuldade de penetração no tecido são utilizados vetores que carreiam os siRNAs até o citoplasma das células, para que os mesmos possam agir(BORGHETI-CARDOSO et al, 2017;DEPIERI et al, 2016;DOWDY, 2017;FOLDVARI et al, 2016;SARETT;NELSON;DUVALL, 2016;TOFANI et al, 2018). Relação de Clinical Trials utilizando siRNAs através ou não de nanopartículas, para tratamento de Câncer (Fonte: clinicaltrials.gov), N.A= não se aplica, palavras-chave da busca: Cancer e siRNA.Em muitos tumores, incluindo os cutâneos, o processo de morte é uma importante via.…”

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Carreador lipídico nanoestruturado multifuncional contendo 5-fluorouracil e siRNA para tratamento de câncer de pele

Rosa¹

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RNAi mediated IL-6 in vitro knockdown in psoriasis skin model with topical siRNA delivery system based on liquid crystalline phase

Cited by 52 publications

References 52 publications

Effects of nanoparticles with hydrotropic nicotinamide on tacrolimus: permeability through psoriatic skin and antipsoriatic and antiproliferative activities

Effects of nanoparticles with hydrotropic nicotinamide on tacrolimus: permeability through psoriatic skin and antipsoriatic and antiproliferative activities

Local co-administration of gene-silencing RNA and drugs in cancer therapy: State-of-the art and therapeutic potential

Carreador lipídico nanoestruturado multifuncional contendo 5-fluorouracil e siRNA para tratamento de câncer de pele

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