RNase Activities Are Reduced Concomitantly with Conservation of Total Cellular RNA and Ribosomes in O2-Deprived Seedling Roots of Maize

Fennoy, Sheila L.; Jayachandran, S.; Bailey‐Serres, Julia

doi:10.1104/pp.115.3.1109

Cited by 22 publications

(15 citation statements)

References 33 publications

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“…Modulation of RNase activities has been expected to play a role in the regulation of RNA turnover in response to certain abiotic and biotic cues (Green, 1993;Wilson, 1975). Especially, Fennoy et al (1997) has demonstrated that a specific set of mRNAs could be conserved during O 2 deficiency through modulation of specific RNase activities in response to O 2 deprivation. If specific group of mRNAs is preferentially stabilized under anoxia by such a decrease in specific RNase activity, the resultant pattern of response would resemble that observed for the type-3 genes in Fig.…”

Section: Figmentioning

confidence: 99%

Differential Anoxic Expression of Sugar-Regulated Genes Reveals Diverse Interactions between Sugar and Anaerobic Signaling Systems in Rice

Lim

Lee

Yim

et al. 2013

Molecules and Cells

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The interaction between the dual roles of sugar as a metabolic fuel and a regulatory molecule was unveiled by examining the changes in sugar signaling upon oxygen deprivation, which causes the drastic alteration in the cellular energy status. In our study, the expression of anaerobically induced genes is commonly responsive to sugar, either under the control of hexokinase or non-hexokinase mediated signaling cascades. Only sugar regulation via the hexokinase pathway was susceptible for O 2 deficiency or energy deficit conditions evoked by uncoupler. Examination of sugar regulation of those genes under anaerobic conditions revealed the presence of multiple paths underlying anaerobic induction of gene expression in rice, subgrouped into three distinct types. The first of these, which was found in type-1 genes, involved neither sugar regulation nor additional anaerobic induction under anoxia, indicating that anoxic induction is a simple result from the release of sugar repression by O 2 -deficient conditions. In contrast, type-2 genes also showed no sugar regulation, albeit with enhanced expression under anoxia. Lastly, expression of type-3 genes is highly enhanced with sugar regulation sustained under anoxia. Intriguingly, the inhibition of the mitochondrial ATP synthesis can reproduce expression pattern of a specific set of anaerobically induced genes, implying that rice cells may sense O 2 deprivation, partly via perception of the perturbed cellular energy status. Our study of interaction between sugar signaling and anaerobic conditions has revealed that sugar signaling and the cellular energy status are likely to communicate with each other and influence anaerobic induction of gene expression in rice.

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Section: Figmentioning

confidence: 99%

Differential Anoxic Expression of Sugar-Regulated Genes Reveals Diverse Interactions between Sugar and Anaerobic Signaling Systems in Rice

Lim

Lee

Yim

et al. 2013

Molecules and Cells

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“…Polysomes were isolated from cytoplasmic extracts by sucrose sedimentation. We used methods derived from the protocols developed for nuclei isolation by Blobel and Potter (1966), for cytoplasmic RNA prepara-tion by del Prete et al (2007), and for polysome isolation by Fennoy et al (1997) with minor modifications.…”

Section: Preparation Of Nuclear Cytoplasmic and Polysomal Rnasmentioning

confidence: 99%

PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Ebralidze¹,

Guibal²,

Steidl³

et al. 2008

Genes Dev.

166

147

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The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.[Keywords: Noncoding antisense RNA; upstream and intronic regulatory elements; coordinated expression of the target and regulator; translation stalling] Supplemental material is available at http://www.genesdev.org.

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“…An effect on the activity of RNases has also been observed in plants, following abiotic stresses such as ozone and salinity (Gomes-Filho and Sodek, 1988;Booker, 2004). An observed decrease in RNase activity in wheat (Triticum aestivum) following heat shock was hypothesized to have a role in the observed increase in mRNA stability (Chang and Gallie, 1997), whereas reduction in activity of specific RNases in response to low-O 2 stress in maize (Zea mays) roots was suggested to facilitate conservation of nontranslating ribosomes and poorly translated mRNA (Fennoy et al, 1997).…”

mentioning

confidence: 99%

Suppression of LX Ribonuclease in Tomato Results in a Delay of Leaf Senescence and Abscission

et al. 2006

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Although present in different organisms and conserved in their protein sequence, the biological functions of T2 ribonucleases (RNase) are generally unknown. Tomato (Lycopersicon esculentum) LX is a T2/S-like RNase and its expression is known to be associated with phosphate starvation, ethylene responses, and senescence and programmed cell death. In this study, LX function was investigated using antisense tomato plants in which the LX protein level was reduced. LX protein levels normally become elevated when leaves senesce and antisense inhibition of LX retarded the progression of senescence. Moreover, we observed a marked delay of leaf abscission in LX-deficient plants. This correlated with specific induction of LX protein in the tomato mature abscission zone tissue. LX RNase gene regulation and the consequences of antisense inhibition indicate that LX has an important functional role in both abscission and senescence.

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RNase Activities Are Reduced Concomitantly with Conservation of Total Cellular RNA and Ribosomes in O2-Deprived Seedling Roots of Maize

Cited by 22 publications

References 33 publications

Differential Anoxic Expression of Sugar-Regulated Genes Reveals Diverse Interactions between Sugar and Anaerobic Signaling Systems in Rice

Differential Anoxic Expression of Sugar-Regulated Genes Reveals Diverse Interactions between Sugar and Anaerobic Signaling Systems in Rice

PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Suppression of LX Ribonuclease in Tomato Results in a Delay of Leaf Senescence and Abscission

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