RNase III and RNase E Influence Posttranscriptional Regulatory Networks Involved in Virulence Factor Production, Metabolism, and Regulatory RNA Processing in Bordetella pertussis

Ifill, Gyles; Blimkie, Travis M.; Lee, Ahwon; Mackie, George A.; Chen, Qing; Stibitz, Scott; Hancock, Robert E. W.; Fernandez, Rachel C.

doi:10.1128/msphere.00650-21

Cited by 5 publications

(1 citation statement)

References 77 publications

(133 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the development of low‐cost high throughput RNA sequencing (RNA‐seq) methods in the beginning of the early 2000s and its applications revealed new functions of RNase III. RNase III was previously known to primarily affect maturation of ribosomal and transfer RNAs, but the spectrum of other RNAs recognised by RNase III has been largely expanded in the last decades (Altuvia et al, 2018; Gatewood et al, 2012; Ifill et al, 2021; Rath et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

RNase III participates in control of quorum sensing, pigmentation and oxidative stress resistance in Rhodobacter sphaeroides

Börner,

Friedrich,

Klug

2023

Molecular Microbiology

View full text Add to dashboard Cite

RNase III is a dsRNA‐specific endoribonuclease, highly conserved in bacteria and eukarya. In this study, we analysed the effects of inactivation of RNase III on the transcriptome and the phenotype of the facultative phototrophic α‐proteobacterium Rhodobacter sphaeroides. RNA‐seq revealed an unexpectedly high amount of genes with increased expression located directly downstream to the rRNA operons. Chromosomal insertion of additional transcription terminators restored wild type‐like expression of the downstream genes, indicating that RNase III may modulate the rRNA transcription termination in R. sphaeroides. Furthermore, we identified RNase III as a major regulator of quorum‐sensing autoinducer synthesis in R. sphaeroides. It negatively controls the expression of the autoinducer synthase CerI by reducing cerI mRNA stability. In addition, RNase III inactivation caused altered resistance against oxidative stress and impaired formation of photosynthetically active pigment‐protein complexes. We also observed an increase in the CcsR small RNAs that were previously shown to promote resistance to oxidative stress. Taken together, our data present interesting insights into RNase III‐mediated regulation and expand the knowledge on the function of this important enzyme in bacteria.

show abstract