RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma

Abstract

Ring finger protein 113A (RNF113A) possesses a C3HC4 zinc finger domain and this domain is found in E3 ubiquitin ligase and is involved in tumorigenesis. To date, and at least to the best of our knowledge, there are no studies available which have investigated RNF113A in cancer. Thus, this study aimed to explore the role of RNF113A in the development of esophageal squamous cell carcinoma (ESCC). For this purpose, paraffin-embedded samples from 117 patients with ESCC were selected, as well as 41 pairs of fresh-frozen ESCC and adjacent normal tissue samples. RNF113A expression was examined by immunohistochemistry and reverse transcription-quantitative PCR (RT-qPCR). RNF113A was overexpressed or silenced in the EC9706 and Eca109 cells. The cells were examined for cell cycle progression, apoptosis, invasiveness and migration. Xenograft tumors were also created in mice using the Eca109 cells. Tumor differentiation (P=0.008) and T classification (P<0.001) were found to be significantly associated with RNF113A expression. No statistically significant association was observed between RNF113A expression and sex, age, histological type, tumor location and lymph node metastasis (N classification). Kaplan-Meier analysis revealed that the patients with ESCC with ahigh expression of RNF113A had a lower survival rate than those with a low expression (P=0.002). Multivariate analysis revealed that RNF113A expression (HR=2.406; 95% CI, 1.301-4.449, P=0.005) was independently associated with overall survival in patients with ESCC. The overexpression of RNF113A promoted proliferation, migration, and invasiveness of ESCC cell lines in vitro, and RNF113A silencing reversed these malignant behaviors. RNF113A knockdown inhibited tumor growth in vivo. Thus, these results indicate that RNF113A promotes the proliferation, migration and invasiveness of ESCC cell lines. RNF113A expression in ESCC is this associated with a poor prognosis of affected patients.

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer

“…KIF15 may be a potential prognostic biomarker in bladder cancer [23]. RNF113A expression is correlated with a poor prognosis of esophageal squamous cell carcinoma [24]. GJB6 involves in glioma cell growth and proliferation, and DNA damage.…”

Gene expression analyses identify a relationship between stanniocalcin 2 and the malignant behavior of colorectal cancer

“… 36 As an oncoprotein, RNF113A promoted cell proliferation, migration, and invasiveness in esophageal squamous cell carcinoma (ESCC). 37 In the study of genome-wide gene expression profiling of CRC and adjacent tissues by Sun and Qian, 38 UTP3 was identified as 1 of 20 hub genes in CRC. It suggested that UTP3 plays a vital role in the development of CRC.…”

Downregulation of long non‐coding RNA LOC101928477 correlates with tumor progression by regulating the epithelial‐mesenchymal transition in esophageal squamous cell carcinoma

“…Briefly, specimens were incubated overnight at 4°C with antibodies specific to Ki‐67 (1:100; Abcam) and MMP10 (1:100; Abcam) in an avidin‐biotin complex method. The signal was later amplified and visualized with a secondary antibody (Beyotime), followed by 3′‐diaminobenzidine chromogen 7,30,31 …”

“…After 24 h incubation, nonmigrated/invaded cells on the upper surface were removed with a cotton swab and cells migrated to the lower chamber were fixed with 4% methanol and stained with crystal violet. The number of migrated cells was recorded under a microscope (DM IL, Leica Microsystems) in five random fields and calculated as the average per field 2,7,31 …”

“…In addition, the incidence of ESCC continues to rise rapidly each year 5 . So far, the prognosis of ESCC patients is still extremely poor 6 and the underlying molecular features of ESCC invasion and metastasis remains to be elucidated 7,8 …”