2018
DOI: 10.1016/j.microc.2016.11.005
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Robust and sensitive LC/MS-MS method for simultaneous detection of acetylsalicylic acid and salicylic acid in human plasma

Abstract: Highlights:• A LC-MS/MS method was developed and validated for simultaneous determination of aspirin and salicylic acid in human plasma.• The accuracy and the precision of the method with one-step liquid-liquid extraction were excellent for both analytes.• The method was highly robust and suitable for the analysis of > 2000 samples in a pharmacokinetic study.• The present method can contribute to the improvement of ASP/SAL determination in patients under antithrombotic therapy.• The method can contribute to th… Show more

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Cited by 27 publications
(17 citation statements)
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“…This is mainly done by drug screening via blood or urine chemical tests. The most widely used methods for detection of pharmaceuticals or their metabolites in blood are liquid chromatography tandem mass spectrometry and gas chromatography coupled with mass spectrometry . These methods are quite sensitive and allow the detection of SA with concentration down to 0.04 mg/l (30 μM).…”
Section: Introductionmentioning
confidence: 99%
“…This is mainly done by drug screening via blood or urine chemical tests. The most widely used methods for detection of pharmaceuticals or their metabolites in blood are liquid chromatography tandem mass spectrometry and gas chromatography coupled with mass spectrometry . These methods are quite sensitive and allow the detection of SA with concentration down to 0.04 mg/l (30 μM).…”
Section: Introductionmentioning
confidence: 99%
“…At the end of intervention, a reduction of daily energy intake and a macronutrient redistribution toward the recommended range were observed. In particular, fiber intake (g/1000 kcal) at baseline was lower than the Italian Dietary Reference Values, and had increased by the end of intervention ( p < 0.001) to reach the reference values [24] (Table 1). An increase of FV intake was observed at the end of intervention ( p < 0.001).…”
Section: Resultsmentioning
confidence: 99%
“…Fasting blood samples were taken on the day after the dietary record was completed, at 8 h ± 30 min a.m., since it has been observed that circulating SA is significantly related both to daily fruit and vegetables (FV) intake of the entire previous week and of the last day [23]. Measurement of the SA serum concentration was performed using isotope dilution liquid chromatography tandem mass spectrometry (ID-LC-MS-MS), optimizing the method described by Sirok et al [24]. In brief, the instrument was a Dionex 3000 UltiMate HPLC (Thermo Fisher Scientific, Rodano (MI), Italy) connected to an AB Sciex 3200 QTRAP LC-MS/MS with electrospray ionization (ESI) TurboIonSpray™ source (AB Sciex S.r.l., Milano, Italy).…”
Section: Methodsmentioning
confidence: 99%
“…ASA and SA were quantified in plasma by isotope dilution LC/ MS/MS, following the method by Sirok et al 34 with some modifications. The analysis was performed in negative multiple reaction monitoring; ASA-d4 and SA-d4 were used as internal standards.…”
Section: Measurement Of Plasma Asa and Samentioning
confidence: 99%