Robust CRISPR/Cas9 mediated genome editing and its application in manipulating plant height in the first generation of hexaploid Ma bamboo (<i>Dendrocalamus latiflorus Munro</i>)

Ye, Shanwen; Chen, Gang; Kohnen, Markus V.; Wang, Wenjia; Cai, Changyang; Ding, WenSha; Wu, Chu; Gu, Lianfeng; Yu-shan, Zheng; Ma, Xiao; Lin, Chentao; Zhu, Qiang

doi:10.1111/pbi.13320

Cited by 42 publications

(32 citation statements)

References 11 publications

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“…8 ). The phytohormone GA has been reported to be involved in skotomorphogenesis, and the GA concentration reached its maximum in the basal portion of moso bamboo [ 45 , 65 ]. Most PhAP s also positively or negatively respond to GA treatment (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots

Wang

Yan

et al. 2021

BMC Genomics

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Background Aspartic proteases (APs) are a class of aspartic peptidases belonging to nine proteolytic enzyme families whose members are widely distributed in biological organisms. APs play essential functions during plant development and environmental adaptation. However, there are few reports about APs in fast-growing moso bamboo. Result In this study, we identified a total of 129 AP proteins (PhAPs) encoded by the moso bamboo genome. Phylogenetic and gene structure analyses showed that these 129 PhAPs could be divided into three categories (categories A, B and C). The PhAP gene family in moso bamboo may have undergone gene expansion, especially the members of categories A and B, although homologs of some members in category C have been lost. The chromosomal location of PhAPs suggested that segmental and tandem duplication events were critical for PhAP gene expansion. Promoter analysis revealed that PhAPs in moso bamboo may be involved in plant development and responses to environmental stress. Furthermore, PhAPs showed tissue-specific expression patterns and may play important roles in rapid growth, including programmed cell death, cell division and elongation, by integrating environmental signals such as light and gibberellin signals. Conclusion Comprehensive analysis of the AP gene family in moso bamboo suggests that PhAPs have experienced gene expansion that is distinct from that in rice and may play an important role in moso bamboo organ development and rapid growth. Our results provide a direction and lay a foundation for further analysis of plant AP genes to clarify their function during rapid growth.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots

Wang

Yan

et al. 2021

BMC Genomics

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“…Latest advancements in gene editing technologies have found increasing use of clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR‐associated endonuclease (Cas9) system in several crop species (Doudna & Charpentier, 2014). Very recently, Ye, Chen, et al (2019) established a CRISPR/Cas9 system in ma bamboo by targeting all homo‐alleles or specifically one allele of a gene. Using this technology, they could demonstrate the plant height changes by knocking out one gibberellin‐responsive gene in ma bamboo.…”

Section: Transgenic Approaches To Crop Improvementmentioning

confidence: 99%

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

Ramakrishnan

Yrjälä

Vinod

et al. 2020

Food and Energy Security

101

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“…Rg3039 is a DcpS inhibitor originally used for the treatment of spinal muscular atrophy. It shows anti-leukemic activity by inducing pre mRNA mismatch, so we can expand its application in AML treatment in the next step [6].…”

Section: The Application Of Crispr / Cas9 Library Technology In the Rmentioning

confidence: 99%

“…This system of bacteria is considered as the adaptive immune system [4]. The CRISPR Cas9 technology was first used in mammalian cells in 2013 and has since been named the "top 10 technological breakthroughs of 2015" by Science [5,6].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 high-throughput screening in cancer research

Liu¹

2020

E3S Web Conf.

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In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely used in various models, including cell lines of mice and zebrafish. The use of CRISPR system to construct a genomic library for high-throughput screening is the main strategy for research of disease, especially tumor target gene research. This article reviews the basic principles and latest developments of CRISPR/Cas9 library screening technology strategies to improve its off-target effect, the basic workflow of library screening, and its application in tumor research.

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Robust CRISPR/Cas9 mediated genome editing and its application in manipulating plant height in the first generation of hexaploid Ma bamboo (Dendrocalamus latiflorus Munro)

Cited by 42 publications

References 11 publications

Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots

Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

CRISPR/Cas9 high-throughput screening in cancer research

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