Robust Electrochemical Biosensors Based on Antifouling Peptide Nanoparticles for Protein Quantification in Complex Biofluids

Song, Zhen; Li, Yang; Li, Rong; Fan, Gao-Chao; Luo, Xiliang

doi:10.1021/acssensors.3c02706

Cited by 8 publications

(1 citation statement)

References 40 publications

(55 reference statements)

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“…The sensitivity of the proposed immunosensing approach was compared to some previous electrochemical CEA biosensors based on amplification-free, − and amplification detection approaches. − As depicted in Table , our proposed biosensor exhibited excellent performance regarding its detection limit and analytical range, which either exceeded or matched the performance of the mentioned examples. It is particularly advantageous that our approach does not require labeling or successive steps of isothermal enzymatic signal amplification, thus reducing the potential for errors and simplifying the detection process.…”

Section: Resultsmentioning

confidence: 93%

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Bahri,

Fredj,

Qin

et al. 2024

ACS Appl. Nano Mater.

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Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the everincreasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL −1 , the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL −1 , surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF's large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.

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Section: Resultsmentioning

confidence: 93%

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Bahri,

Fredj,

Qin

et al. 2024

ACS Appl. Nano Mater.

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Breaking barriers in electrochemical biosensing using bioinspired peptide and phage probes

Campuzano,

Pedrero,

Barderas

et al. 2024

Anal Bioanal Chem

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Electrochemical biosensing continues to advance tirelessly, overcoming barriers that have kept it from leaving research laboratories for many years. Among them, its compromised performance in complex biological matrices due to fouling or receptor stability issues, the limitations in determining toxic and small analytes, and its use, conditioned to the commercial availability of commercial receptors and the exploration of natural molecular interactions, deserved to be highlighted. To address these challenges, in addition to the intrinsic properties of electrochemical biosensing, its coupling with biomimetic materials has played a fundamental role, among which bioinspired phage and peptide probes stand out. The versatility in design and employment of these probes has opened an unimaginable plethora of possibilities for electrochemical biosensing, improving their performance far beyond the development of highly sensitive and selective devices. The state of the art offers robust electroanalytical biotools, capable of operating in complex samples and with exciting opportunities to discover and determine targets regardless of their toxicity and size, the commercial availability of bioreceptors, and prior knowledge of molecular interactions. With all this in mind, this review offers a panoramic, novel, and updated vision of both the tremendous advances and opportunities offered by the combination of electrochemical biosensors with bioinspired phage and peptide probes and the challenges and research efforts that are envisioned in the immediate future. Graphical Abstract

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Double hook-type aptamer-based colorimetric and electrochemical biosensor enables rapid and robust analysis of EpCAM expression

Hu,

Yang,

Chen

et al. 2024

Biosensors and Bioelectronics

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Robust Electrochemical Biosensors Based on Antifouling Peptide Nanoparticles for Protein Quantification in Complex Biofluids

Cited by 8 publications

References 40 publications

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Breaking barriers in electrochemical biosensing using bioinspired peptide and phage probes

Double hook-type aptamer-based colorimetric and electrochemical biosensor enables rapid and robust analysis of EpCAM expression

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