Robust enhancing stability and fructose tolerance of sucrose phosphorylase by immobilization on Ni-NTA functionalized agarose microspheres for the biosynthesis of 2-α-glucosylglycerol

Mediated electron transfer in a photo-bioreactor: continuous flow hydroxylation using cytochrome P450 BM3 in NADPH-free conditions

“…54 In this study, two supports were tested for the immobilization of P450 BM3: agarose gel beads activated with Ni-chelated nitrilotriacetic acid (Ni-NTA) and Sepharose resin beads activated with Ni-chelated iminodiacetic acid (Ni-IDA); these supports allow direct, selective immobilization of His-tagged enzyme from clarified cell lysate, enabling efficient immobilization of P450 BM3 for further use in biocatalysis. 55,56 Ni-NTA beads have been shown to be effective in immobilizing a range of enzymes, including lipases, proteases, and oxidoreductases, for various applications such as bio-transformations, biosensing, and drug discovery. For example, immobilized P450 monooxygenase on Ni-NTA beads for the selective hydroxylation of steroids retained its activity and showed improved stability and selectivity compared to the free enzyme.…”