Robust enzymes designing for efficient biocatalysis

Parra‐Saldívar, Roberto; Ramirez‐Mendoza, Ricardo A.; Sharma, Ashutosh; Oza, Goldie; Zavala-Yoé, Ricardo; Iqbal, Hafiz M.N.

doi:10.1016/b978-0-12-819820-9.00004-1

Cited by 9 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Actinobacteria are interesting targets for chiral catalytic enzymes [55] because they are the richest bioactive sources [56] . By referring particularly to actinobacteria isolated from extreme environments, they are expected to harbor robust enzymes with unique features [56] .…”

Section: Stereo‐selective Reactions Catalyzed By Enzymes Derived From...mentioning

confidence: 99%

Actinobacteria as Microbial Cell Factories and Biocatalysts in The Synthesis of Chiral Intermediates and Bioactive Molecules; Insights and Applications

Salama,

Mostafa,

Husseiny

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

Actinobacteria are one of the most intriguing bacterial phyla in terms of chemical diversity and bioactivities of their reported biomolecules and natural products, including various types of chiral molecules. Actinobacterial genera such as Detzia, Mycobacterium, and Streptomyces are among the microbial sources targeted for selective reactions such as asymmetric biocatalysis catalyzed by whole cells or enzymes induced in their cell niche. Remarkably, stereoselective reactions catalyzed by actinobacterial whole cells or their enzymes include stereoselective oxidation, stereoselective reduction, kinetic resolution, asymmetric hydrolysis, and selective transamination, among others. Species of actinobacteria function with high chemo‐, regio‐, and enantio‐selectivity under benign conditions, which could help current industrial processing. Numerous selective enzymes were either isolated from actinobacteria or expressed from actinobacteria in other microbes and hence exploited in the production of pure organic compounds difficult to obtain chemically. In addition, different species of actinobacteria, especially Streptomyces species, function as natural producers of chiral molecules of therapeutic importance. Herein, we discuss some of the most outstanding contributions of actinobacteria to asymmetric biocatalysis, which are important in the organic and/or pharmaceutical industries. In addition, we highlight the role of actinobacteria as microbial cell factories for chiral natural products with insights into their various biological potentialities.

show abstract

Section: Stereo‐selective Reactions Catalyzed By Enzymes Derived From...mentioning

confidence: 99%

Actinobacteria as Microbial Cell Factories and Biocatalysts in The Synthesis of Chiral Intermediates and Bioactive Molecules; Insights and Applications

Salama,

Mostafa,

Husseiny

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…These methods are based on three main processes: biosorption, bioaccumulation, and biodegradation, or a combination of these. Regarding biodegradation, an enzyme participates in the conversion of the pollutants to simpler compounds usually with less toxicity than the initial ones [21,32,33]. The use of native enzymes to degrade pollutants is subject to certain limitations, such as low stability and difficulty of recovery, which impair their reusability.…”

Section: Applications Of Immobilized Enzymes For Removing Water Pollutantsmentioning

confidence: 99%

Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

Kołodziejczak-Radzimska

Nghiem

Jesionowski

2021

Curr Pollution Rep

View full text Add to dashboard Cite

Purpose of Review Untreated wastewater discharge can significantly and negatively impact the state of the environment. Rapid industrialization and economic development have directly contributed to land and water pollution resulting from the application of many chemicals such as organic dyes, pharmaceuticals, and industrial reagents. The removal of these chemicals before effluent discharge is crucial for environmental protection. This review aims to explore the importance of functionalized materials in the preparation of biocatalytic systems and consider their application in eliminating water pollutants. Recent Findings Wastewater treatment methods can be classified into three groups: (i) chemical (e.g., chemical oxidation and ozonation), (ii) physical (e.g., membrane separation and ion exchange), and (iii) biological processes. Biological treatment is the most widely used method due to its cost-effectiveness and eco-friendliness. In particular, the use of immobilized enzymes has recently become more attractive as a result of scientific progress in advanced material synthesis. The selection of an appropriate support plays an important role in the preparation of such biologically active systems. Recent studies have demonstrated the use of various materials for enzyme immobilization in the purification of water. Summary This review identifies and discusses different biocatalytic systems used in the enzymatic degradation of various water pollutants. Materials functionalized by specific groups can serve as good support matrices for enzyme immobilization, providing chemical and thermal stability to support catalytic reactions. Enzymatic biocatalysis converts the pollutants into simpler products, which are usually less toxic than their parents. Due to immobilization, the enzyme can be used over multiple cycles to reduce the cost of wastewater treatment. Future studies in this field should focus on developing new platforms for enzyme immobilization in order to improve degradation efficiency.

show abstract

“…For successful engineering of any protein it requires advance information regarding its amino acid concatenation, structural geometry, active site, subsite and loops forming residues near catalytic core and folds and networks of bonds, along with reaction mechanism [ [70] , [71] , [72] ]. Protein engineering is the espoused method for modifying CAZyme for improved activity and stability together with augmentation of the products biosynthesis.…”

Section: Engineering Of Cazyme For Improvement Of Propertiesmentioning

confidence: 99%

“…Both techniques have their own advantages and thus combining both the techniques can be possibly more effective in enhancing the desired enzyme properties [ 116 ]. Additionally, newly emerging protein engineering techniques such as nanomaterials-based enzyme immobilization, advanced computational modelling, microenvironment engineering via substrate engineering are being exploited for developing multifunctional enzymes [ 72 ].…”

Section: Engineering Of Cazyme For Improvement Of Propertiesmentioning

confidence: 99%

Innovations in CAZyme gene diversity and its modification for biorefinery applications

Chettri

Verma

2020

Biotechnology Reports

View full text Add to dashboard Cite

Highlights The review analyses the role of CAZymes in lignocellulose degradation with focus on the different approaches for improving the enzyme activity. Omics based approaches for the analysis of genes, transcripts, proteins and metabolites related to plant degrading mechanism. Directed evolution and rational design as approaches for engineering to enhancing the CAZyme properties. Microbial engineering of the fungal and microbial strains by adapting techniques like CRISPR/cas9 and use of various expression systems. Novel enzymes and cocktail formulation for biorefinery-based application for production of value-added products using low cost plant biomass.

show abstract

Robust enzymes designing for efficient biocatalysis

Cited by 9 publications

References 56 publications

Actinobacteria as Microbial Cell Factories and Biocatalysts in The Synthesis of Chiral Intermediates and Bioactive Molecules; Insights and Applications

Actinobacteria as Microbial Cell Factories and Biocatalysts in The Synthesis of Chiral Intermediates and Bioactive Molecules; Insights and Applications

Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

Innovations in CAZyme gene diversity and its modification for biorefinery applications

Contact Info

Product

Resources

About