Robust naphthyl methacrylate monolithic column for high performance liquid chromatography of a wide range of solutes

Jonnada, Murthy; Rassi, Ziad El

doi:10.1016/j.chroma.2015.07.069

Cited by 19 publications

(12 citation statements)

References 22 publications

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“…biphenyl ligands, on the surface of the OHM precursor. Through the course of monolithic column development, aromatic acrylate monolithic capillary columns emerged , and more recently, other promising aromatic monolithic stationary phases appeared . This is not surprising since there has been a noticeable rise in the commercial availability of phenyl type reversed‐phase columns for HPLC separations due to their unique retention mechanism.…”

Section: Introductionmentioning

confidence: 99%

Postpolymerization modification of a hydroxy monolith precursor. Part II. Epoxy biphenyl modified poly (hydroxyethyl methacrylate‐co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatography based on π–π and hydrophobic interactions

Khadka

Rassi

2016

Electrophoresis

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In this second part of the series of investigations involving the postpolymerization modification of a hydroxy monolith (OHM) capillary column, the surface hydroxyl groups were reacted with epoxy biphenyl thus yielding the so-called Biphenyl OHM capillary column. The modification involved the epoxy ring opening of the 2-biphenylyl glycidyl ether catalyzed by BF and its subsequent reaction with the hydroxyl groups on the OHM precursor surface. The Biphenyl OHM capillary column thus obtained exhibited the typical reversed phase behavior by primarily hydrophobic interactions vis-à-vis the homologous series of alkyl benzenes and in addition by π-π interactions toward nitroalkane homologous series via their π-electron rich nitro groups. This dual retention mechanism was very distinctly observed with a set of PAH solutes in the sense that the k values of the PAH solutes were comparable to those obtained on a more non polar stationary phase, namely the Epoxy OHM C-16 reported in the preceding article. Other aromatic solutes showed the dual retention mechanism on the Biphenyl OHM capillary including phenols, anilines derivatives, and phenoxy acid herbicides. The Biphenyl OHM capillary exhibited good reproducibility from run-to-run, day-to-day, and column-to-column.

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Section: Introductionmentioning

confidence: 99%

Postpolymerization modification of a hydroxy monolith precursor. Part II. Epoxy biphenyl modified poly (hydroxyethyl methacrylate‐co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatography based on π–π and hydrophobic interactions

Khadka

Rassi

2016

Electrophoresis

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“…The retention behaviors of FA‐6AQ derivatives were first evaluated on the in‐house made napthyl methacrylate monolithic column (NMM column), which was designed recently in our laboratory, and its usefulness has been demonstrated in the separation of both small and large molecules . In general, monolithic columns are supposed to have a characteristic large pore size, which is appropriate for the separation of FAs especially those with long carbon chains.…”

Section: Resultsmentioning

confidence: 99%

“…Chromatographic separations were performed on a 5 μm wide pore C4 functionalized silica particles column (C4 column), 15 cm × 4.6 mm id, from J.T. Baker (Phillipsburg, NJ, USA), a 5 μm Nucleosil CN functionalized silica (CN column), 15 cm × 4.6 mm id, and an in‐house synthesized naphthyl functionalized organic monolithic column (NMM column), 10 cm × 4.6 mm id .…”

Section: Methodsmentioning

confidence: 99%

Selective precolumn derivatization of fatty acids with the fluorescent tag 6‐aminoquinoline and their determination in some food samples by reversed‐phase chromatography

Jonnada

Rassi

2017

Electrophoresis

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Fatty acids (FAs) have been selectively derivatized with a fluorescent tag, 6-aminoquinoline (6AQ), which yielded fluorescent FA-6AQ derivatives that have excitation (λ = 270 nm) and emission (λ = 495 nm) wavelengths that are farther apart. This precolumn derivatization is characterized by its simplicity occurring at room temperature between the carboxylic acid group of the FA and the amino group of 6AQ in the presence of a nonaqueous soluble carbodiimide coupling agent such as the N,N´-dicyclohexylcarbodiimide. The FAs extracts are readily derivatized in chloroform and can be analyzed without any further sample cleanup that minimizes sample loss. The FA-6AQ derivatives derived from standard FAs as well as from extracted FAs from food samples were separated by reversed phase chromatography on a homemade naphthyl methacrylate monolithic (NMM) column and C4 silica-based column. While the NMM column provided excellent separation for saturated FA-6AQ derivatives, the C4 silica column was able to separate simultaneously saturated and unsaturated FA-6AQ derivatives. The MNN column permitted the analysis and quantitation of the saturated FA-6AQ derivatives extracted from coconut oil. The C4 column provided the selectivity needed to analyze and quantify saturated and unsaturated derivatized with 6AQ and extracted from meat. The limits of detection and quantitation were 5 and 20 nM, respectively, with a linear dynamic range extending from 20 nM to 40 μM. The 40 μM upper limit was due to the limited solubility of the FA-6AQ derivatives in the diluting mobile phase, which is the initial mobile phase used in gradient runs.

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“…Recently, Jonnada and El Rassi have introduced an organic monolithic column by the in situ copolymerization of 2‐naphthyl methacrylate as the functional monomer and trimethylolpropane trimethacrylate (TRIM) as the cross‐linker in a stainless steel column of 4.6 mm id for RPC . The column showed high mechanical stability and good hydrodynamic characteristics with virtually “zero” compressibility over a long period of daily use with a wide range of mobile phase compositions.…”

Section: Nonpolar Organic Monolithsmentioning

confidence: 99%

“…Gradient elution: 5–60% B in 30 min (A); 10–80% B in 10 min (B); 15–60% B in 1 min (C). Peaks: (1) ribonuclease A, (2) cytochrome C, (3) lysozyme, (4) β‐lactoglobulin A, (5) myoglobin, (6) α‐chymotrypsinogen A. Reproduced with permission from .…”

Section: Nonpolar Organic Monolithsmentioning

confidence: 99%

Polar and nonpolar organic polymer‐based monolithic columns for capillary electrochromatography and high‐performance liquid chromatography

et al. 2016

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This review article is a continuation of the previous reviews on the area of monolithic columns covering the progress made in the field over the last couple of years from the beginning of the second half of 2014 until the end of the first half of 2016. It summarizes and evaluates the evolvement of both polar and nonpolar organic monolithic columns and their use in hydrophilic interaction LC and CEC and reversed-phase chromatography and RP-CEC. The review article discusses the results reported in a total of 62 references.

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Robust naphthyl methacrylate monolithic column for high performance liquid chromatography of a wide range of solutes

Cited by 19 publications

References 22 publications

Postpolymerization modification of a hydroxy monolith precursor. Part II. Epoxy biphenyl modified poly (hydroxyethyl methacrylate‐co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatography based on π–π and hydrophobic interactions

Postpolymerization modification of a hydroxy monolith precursor. Part II. Epoxy biphenyl modified poly (hydroxyethyl methacrylate‐co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatography based on π–π and hydrophobic interactions

Selective precolumn derivatization of fatty acids with the fluorescent tag 6‐aminoquinoline and their determination in some food samples by reversed‐phase chromatography

Polar and nonpolar organic polymer‐based monolithic columns for capillary electrochromatography and high‐performance liquid chromatography

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