Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells

Suzuki, Atsushi; Iwama, Atsushi; Matsumoto, Hitoshi; Nakauchi, Hiromitsu; Taniguchi, Hideki

doi:10.1242/dev.00459

Cited by 160 publications

(124 citation statements)

References 45 publications

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“…Suzuki et al (2003) showed that type I collagen played a supportive role in hepatocytic differentiation in a murine system. We did not observe a similar effect in the chick explant culture.…”

Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 99%

“…Second, it may be a result of experimental variables. In the present study, whole liver buds of HH16 chick embryos were used, whereas Suzuki et al (2003) used a rare subpopulation of E13.5 mouse hepatoblasts, which they isolated using several cell-surface markers. This suggests that the diverse differentiation responses to type I collagen may be the result of experimental variables and not species-specific characteristics.…”

Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 99%

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FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Yanai

Tatsumi

Hasunuma

et al. 2008

Developmental Dynamics

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Intrahepatic bile ducts (IHBDs) are indispensable for transporting bile secreted from hepatocytes to the hepatic duct. The biliary epithelial cells (BECs) of the IHBD arise from bipotent hepatoblasts around the portal vein, suggesting the portal mesenchyme is essential for their development. However, except for Notch or Activin/ TGF-␤ signaling molecules, it is not known which molecules regulate IHBD development. Here, we found that FGF receptors and BMP4 are specifically expressed in the developing IHBD and the hepatic mesenchyme, respectively. Using a mesenchyme-free culture of liver bud, we showed that bFGF and FGF7 induce the hepatoblasts to differentiate into BECs, and that BMP4 enhances bFGF-induced BEC differentiation. The extracellular matrix (ECM) components in the hepatic mesenchyme induced BEC differentiation. Forced expression of a constitutively active form of the FGF receptor partially induced BEC differentiation markers in vivo. These data strongly suggest that bFGF and FGF7 promote BEC differentiation cooperatively with BMP4 and ECMs in vivo.

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Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 99%

Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 99%

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Yanai

Tatsumi

Hasunuma

et al. 2008

Developmental Dynamics

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“…ECM serves as a reservoir of biologically active soluble molecules, including cytokines that regulate HSC activation. Different structural matrix components also have the potential to stimulate or curtail HSC activation (38,43), and interactions between ECM composition and cell phenotype have been identified in a variety of tissues (22,39,42).…”

mentioning

confidence: 99%

Differences in regulation of type I collagen synthesis in primary and passaged hepatic stellate cell cultures: the role of α₅β₁-integrin

Dodig¹,

Ogunwale²,

Dasarathy³

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

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Hepatic stellate cells (HSC) differ in their phenotype depending on the initiation and progression of their activation. Our hypothesis was that different mechanisms govern type I collagen synthesis depending on stage of HSC activation. We investigated the role of ␣5␤1-integrin as a regulator of type I collagen gene COL1A1 expression in primary and passaged HSC cultures using transgenic mouse containing type I collagen gene COL1A1 promoter linked to the chloramphenicol acetyltransferase (CAT) reporter gene. The ␣5␤1 protein levels increased during the activation and were highest in day 6 primary cultures but decreased in passaged HSC. CAT activity, reflecting COL1A1 expression, was upregulated by ␣5␤1-integrin. Inhibition of ␣5␤1-integrin by echistatin and blocking antibody resulted in reduced transgene activity only in early primary cultures (compared with the control, 53.3 Ϯ 12% echistatin and 58.8 Ϯ 7% blocking antibody, respectively, P Ͻ 0.05). Treatment of passaged HSC with either echistatin or blocking antibody had no effect. Fibronectin, an ␣5␤1-integrin ligand, increased transgene activity in primary (210 Ϯ 33%, P Ͻ 0.05) but not in passaged HSC cultures (119 Ϯ 8%). This ␣5␤1-integrin effect appears to be at least in part mediated by CCAAT enhancer binding protein-␤ (C/EBP␤), because fibronectin increased and ␣5-gene silencing by small interfering RNA decreased C/EBP␤ levels. In addition, C/EBP␤ knockout mice showed reduced type I collagen synthesis compared with wild-type littermates. Therefore ␣5␤1-integrin is an important regulator of type I collagen production in early primary HSC cultures but appears to have no direct role once the HSC are fully activated.

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“…There are several advantages in using decellularized organs as a platform for whole liver engineering; the use of the decellularized liver bioscaffold provides not only a 3D‐vascularized scaffold for nutrient delivery but also retains the environmental cues necessary for progenitor hepatic and endothelial cells to grow, differentiate, and maintain functionality 55, 56, 57…”

Section: Decellularized 3d Ecm Scaffolds For Tissue/whole Organ Enginmentioning

confidence: 99%

Liver tissue engineering: From implantable tissue to whole organ engineering

Mazza

Al‐Akkad

Rombouts

et al. 2017

Hepatology Communications

113

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The term “liver tissue engineering” summarizes one of the ultimate goals of modern biotechnology: the possibility of reproducing in total or in part the functions of the liver in order to treat acute or chronic liver disorders and, ultimately, create a fully functional organ to be transplanted or used as an extracorporeal device. All the technical approaches in the area of liver tissue engineering are based on allocating adult hepatocytes or stem cell‐derived hepatocyte‐like cells within a three‐dimensional structure able to ensure their survival and to maintain their functional phenotype. The hosting structure can be a construct in which hepatocytes are embedded in alginate and/or gelatin or are seeded in a pre‐arranged scaffold made with different types of biomaterials. According to a more advanced methodology termed three‐dimensional bioprinting, hepatocytes are mixed with a bio‐ink and the mixture is printed in different forms, such as tissue‐like layers or spheroids. In the last decade, efforts to engineer a cell microenvironment recapitulating the dynamic native extracellular matrix have become increasingly successful, leading to the hope of satisfying the clinical demand for tissue (or organ) repair and replacement within a reasonable timeframe. Indeed, the preclinical work performed in recent years has shown promising results, and the advancement in the biotechnology of bioreactors, ex vivo perfusion machines, and cell expansion systems associated with a better understanding of liver development and the extracellular matrix microenvironment will facilitate and expedite the translation to technical applications. (Hepatology Communications 2018;2:131–141)

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Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells

Cited by 160 publications

References 45 publications

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Differences in regulation of type I collagen synthesis in primary and passaged hepatic stellate cell cultures: the role of α₅β₁-integrin

Liver tissue engineering: From implantable tissue to whole organ engineering

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Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells

Cited by 160 publications

References 45 publications

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Differences in regulation of type I collagen synthesis in primary and passaged hepatic stellate cell cultures: the role of α5β1-integrin

Liver tissue engineering: From implantable tissue to whole organ engineering

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Differences in regulation of type I collagen synthesis in primary and passaged hepatic stellate cell cultures: the role of α₅β₁-integrin