Role of a Ubiquitously Expressed Receptor in the Vertebrate Olfactory System

DeMaria, Shannon; Berke, Allison P.; Name, Eric Van; Heravian, Anisa; Ferreira, Todd; Ngai, John

doi:10.1523/jneurosci.2339-13.2013

Cited by 50 publications

(69 citation statements)

References 59 publications

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“…Calretinin appears to label subpopulations of ciliated and microvillous neurons101516. Again we observe no co-localisation for G o -ir and calretinin (<1%, Fig.…”

Section: Resultsmentioning

confidence: 56%

Kappe neurons, a novel population of olfactory sensory neurons

Ahuja

Nia

Zapilko

et al. 2014

Sci Rep

101

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Perception of olfactory stimuli is mediated by distinct populations of olfactory sensory neurons, each with a characteristic set of morphological as well as functional parameters. Beyond two large populations of ciliated and microvillous neurons, a third population, crypt neurons, has been identified in teleost and cartilaginous fishes. We report here a novel, fourth olfactory sensory neuron population in zebrafish, which we named kappe neurons for their characteristic shape. Kappe neurons are identified by their Go-like immunoreactivity, and show a distinct spatial distribution within the olfactory epithelium, similar to, but significantly different from that of crypt neurons. Furthermore, kappe neurons project to a single identified target glomerulus within the olfactory bulb, mdg5 of the mediodorsal cluster, whereas crypt neurons are known to project exclusively to the mdg2 glomerulus. Kappe neurons are negative for established markers of ciliated, microvillous and crypt neurons, but appear to have microvilli. Kappe neurons constitute the fourth type of olfactory sensory neurons reported in teleost fishes and their existence suggests that encoding of olfactory stimuli may require a higher complexity than hitherto assumed already in the peripheral olfactory system.

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“…Calretinin appears to label subpopulations of ciliated and microvillous neurons101516. Again we observe no co-localisation for G o -ir and calretinin (<1%, Fig.…”

Section: Resultsmentioning

confidence: 56%

Kappe neurons, a novel population of olfactory sensory neurons

Ahuja

Nia

Zapilko

et al. 2014

Sci Rep

101

View full text Add to dashboard Cite

show abstract

“…This study is unique in reporting a ligand for any of the 112 zebrafish TAARs, and following identification of two amino acid-activated receptors from the V2R-related receptor family (24,25), constitutes the third deorphanization of any fish olfactory receptor. TAAR13c is strongly activated by primary amines and indeed is phylogenetically closer to those rodent TAARs that prefer primary over tertiary amines (18).…”

Section: Discussionmentioning

confidence: 99%

High-affinity olfactory receptor for the death-associated odor cadaverine

Hussain

Saraiva

Ferrero

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

172

234

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Carrion smell is strongly repugnant to humans and triggers distinct innate behaviors in many other species. This smell is mainly carried by two small aliphatic diamines, putrescine and cadaverine, which are generated by bacterial decarboxylation of the basic amino acids ornithine and lysine. Depending on the species, these diamines may also serve as feeding attractants, oviposition attractants, or social cues. Behavioral responses to diamines have not been investigated in zebrafish, a powerful model system for studying vertebrate olfaction. Furthermore, olfactory receptors that detect cadaverine and putrescine have not been identified in any species so far. Here, we show robust olfactory-mediated avoidance behavior of zebrafish to cadaverine and related diamines, and concomitant activation of sparse olfactory sensory neurons by these diamines. The large majority of neurons activated by low concentrations of cadaverine expresses a particular olfactory receptor, trace amineassociated receptor 13c (TAAR13c). Structure-activity analysis indicates TAAR13c to be a general diamine sensor, with pronounced selectivity for odd chains of medium length. This receptor can also be activated by decaying fish extracts, a physiologically relevant source of diamines. The identification of a sensitive zebrafish olfactory receptor for these diamines provides a molecular basis for studying neural circuits connecting sensation, perception, and innate behavior.Danio rerio | aversion | heterologous expression | polyamines C adaverine, putrescine, and other biogenic diamines are strongly repulsive odors to humans, for whom these odors presumably signal bacterial contamination. It may be expected that animal species feeding on carcasses attribute a more positive valence to diamines, and indeed both putrescine and cadaverine have been reported to be feeding attractants for rats (1) as well as goldfish (2). Similarly, insects depositing their eggs in carcasses or other proteineacous materials are attracted by these diamines (3). Beyond signaling danger or food, putrescine and cadaverine also serve as social cues in several vertebrate species, both for marking of territories-for example, in feline species (4)-and for burial of conspecifics (5).Very little is known about the molecular and cellular basis of cadaverine-driven behaviors. Cadaverine and putrescine evoke electrophysiological responses in the olfactory epithelium of two fish species (2, 6) and cadaverine-responsive olfactory sensory neurons and glomeruli have been identified in the mouse (7,8). However, chemosensory receptors that detect cadaverine or related diamines are unknown in any species, and could provide valuable tools to study how the olfactory system mediates innate aversion or attraction.Here, we show that cadaverine is a major product of zebrafish tissue decay, activates a zebrafish olfactory receptor (trace amineassociated receptor 13c, TAAR13c) with high affinity, and elicits a strong, low-threshold, and olfactory-mediated avoidance response in zebrafish. In vivo me...

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“…Results from a heterologous HEK293 expression system suggest that Vmn2r1 is a calcium‐dependent, low‐sensitivity receptor for isoleucine, leucine, and valine (DeMaria et al ., ). If these responses can be extended in vivo or ex vivo to VSNs of WT mice and can be shown to be reduced or abolished in the ∆C1 or ∆C1‐GFP background, the reduced cell number could be interpreted in terms of reduced VSN survival due to absent or aberrant physiological responses.…”

Section: Discussionmentioning

confidence: 97%