Role of an Atypical E2F Transcription Factor in the Control of Arabidopsis Cell Growth and Differentiation

Ramírez-Parra, Elena; López-Matas, M. Ángeles; Fründt, Corinne; Gutiérrez, Crisanto

doi:10.1105/tpc.104.023978

Cited by 90 publications

(78 citation statements)

References 61 publications

(100 reference statements)

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“…1E). The opposite effect was observed in plants deficient for E2Fd/DEL2 (e2fd plants) or E2Ff/ DEL3 (e2ff plants; Ramirez-Parra et al, 2004), and in E2Fe/DEL1 OE plants Fig. 1E).…”

Section: Fas1 Is An E2f Target In Vivomentioning

confidence: 92%

“…ChIP assays and data analysis were carried out basically as previously described (Ramirez-Parra et al, 2004), treating plants with 10 mM sodium butirate (Sigma) during 4 h prior to fixation and using anti-HA (Roche), antiMyc 9E10 (Santa Cruz Biotechnology), anti-tetra-AcH4 (Usptate), antidi-AcH3 (Usptate), and anti-di-MetK9H3 (Usptate) antibodies. Sequences of primers used are specified in Supplemental Table S2.…”

Section: Emsa and Chip Assaysmentioning

confidence: 99%

“…EMSAs were carried out as described (Ramirez-Parra et al, 2004) using purified MBP-AtE2Ff and a FAS1 promoter fragment (2370 to 2195 relative to the ATG start codon) or double-stranded oligonucleotides containing E2F sites, E2F1 (5#-CTTGAGTGGCGGCAAACAGTAAAAT-3#) and E2F2 (5#-AAC-CTTGGCGGGAAAATCAACGACC-3#) and their complementary sequences as probes.…”

Section: Emsa and Chip Assaysmentioning

confidence: 99%

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E2F RegulatesFASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status

2007

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Maintenance of genome integrity depends on histone chaperone-mediated chromatin reorganization. DNA replicationassociated nucleosome deposition relies on chromatin assembly factor-1 (CAF-1). Depletion of CAF-1 in human cells leads to cell death, whereas in Arabidopsis (Arabidopsis thaliana), where it is involved in heterochromatin compaction and homologous recombination, plants are viable. The mechanism that makes the lack of CAF-1 activity compatible with development is not known. Here, we show that the FASCIATA1 (FAS1) gene, which encodes the CAF-1 large subunit, is a target of E2F transcription factors. Mutational studies demonstrate that one of the two E2F binding sites in its promoter has an activator role, whereas the other has a repressor function. Loss of FAS1 results in reduced type A cyclin-dependent kinase activity, inhibits mitotic progression, and promotes a precocious and systemic switch to the endocycle program. Selective up-regulation of the expression of a subset of genes, including those involved in activation of the G2 DNA damage checkpoint, also occurs upon FAS1 loss. This activation is not the result of a global change in chromatin structure, but depends on selective epigenetic changes in histone acetylation and methylation within a small region in their promoters. This suggests that correct chromatin assembly during the S-phase is required to prevent unscheduled changes in the epigenetic marks of target genes. Interestingly, activation of the endocycle switch as well as introduction of activating histone marks in the same set of G2 checkpoint genes are detected upon treatment of wild-type plants with DNA-damaging treatments. Our results are consistent with a model in which defects in chromatin assembly during the S-phase and DNA damage signaling share part of a pathway, which ultimately leads to mitotic arrest and triggers the endocycle program. Together, this might be a bypass mechanism that makes development compatible with cell division arrest induced by DNA damage stress.

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“…1E). The opposite effect was observed in plants deficient for E2Fd/DEL2 (e2fd plants) or E2Ff/ DEL3 (e2ff plants; Ramirez-Parra et al, 2004), and in E2Fe/DEL1 OE plants Fig. 1E).…”

Section: Fas1 Is An E2f Target In Vivomentioning

confidence: 92%

Section: Emsa and Chip Assaysmentioning

confidence: 99%

Section: Emsa and Chip Assaysmentioning

confidence: 99%

See 1 more Smart Citation

E2F RegulatesFASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status

2007

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“…ChIP assays and data analysis were carried out basically as described previously (Ramirez-Parra et al, 2004). Briefly, whole 12-DAS wild-type or E2Fd (E2Fd OE ), E2Fe (E2Fe OE ), and E2Ff (E2Ff OE ) overexpressing plants were treated with 1% (v/v) formaldehyde under vacuum.…”

Section: Chip Assaysmentioning

confidence: 99%

“…In addition, chromatin immunoprecipitation (ChIP) experiments were performed using commercial antibodies that recognize hemagglutinin (HA) or anti-myc antibodies with transgenic Arabidopsis plants that overexpress HA-E2Fe (E2Fe OE ), HA-E2Ff (E2Ff OE ; Ramirez-Parra et al, 2004), or myc-E2Fd (E2Fd OE ; Ramirez-Parra and Gutierrez, 2007) fusion proteins. We chose to use these plants because (1) they show high induction (Fig.…”

Section: Asf1a and Asf1b Are Regulated By E2f Transcription Factorsmentioning

confidence: 99%

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

et al. 2013

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ANTI-SILENCING FUNCTION1 (ASF1) is a key histone H3/H4 chaperone that participates in a variety of DNA-and chromatin-related processes, including DNA repair, where chromatin assembly and disassembly are of primary relevance. Information concerning the role of ASF1 proteins in the post-ultraviolet (UV) response in higher plants is currently limited. In Arabidopsis (Arabidopsis thaliana), an initial analysis of in vivo localization of ASF1A and ASF1B indicates that both proteins are mainly expressed in proliferative tissues. In silico promoter analysis identified ASF1A and ASF1B as potential targets of Elongation Factor2 (E2F) transcription factors. These observations were experimentally validated, both in vitro, by electrophoretic mobility shift assays, and in vivo, by chromatin immunoprecipitation assays and expression analysis using transgenic plants with altered levels of different E2F transcription factors. These data suggest that ASF1A and ASF1B are regulated during cell cycle progression through E2F transcription factors. In addition, we found that ASF1A and ASF1B are associated with the UV-B-induced DNA damage response in Arabidopsis. Transcript levels of ASF1A and ASF1B were increased following UV-B treatment. Consistent with a potential role in UV-B response, RNA interference-silenced plants of both genes showed increased sensitivity to UV-B compared with wild-type plants. Finally, by coimmunoprecipitation analysis, we found that ASF1 physically interacts with amino-terminal acetylated histones H3 and H4 and with acetyltransferases of the Histone Acetyl Transferase subfamily, which are known to be involved in cell cycle control and DNA repair, among other functions. Together, we provide evidence that ASF1A and ASF1B are regulated by cell cycle progression and are involved in DNA repair after UV-B irradiation.

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Control of Plant Organ Size

Egea‐Cortines

Weiß

2013

Encyclopedia of Life Sciences

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Lateral aerial organs are produced from the primordia initiated in the shoot apical meristem. Early stages of lateral organ growth require cell division and a sharp transition occurs late in development towards a cell expansion‐based growth. A current hypothesis indicates that cytokinin signalling in meristematic cells is downregulated to allow differentiation. Increased local auxin levels promoting growth accompany this event. The later stage coincides with jasmonic acid signalling required for flower maturarion. Within one organism, lateral organs of the same type can change in size and shape during ontogeny, a feature called heteroblasty. The developmental programme leading to final organ size may be modulated by environmental cues including photoperiod, temperature and abiotic stress. These environmental signals are integrated into the morphogenetic programme allowing better adaptation as a result of developmental plasticity. Key Concepts: Initial growth of lateral organs requires the downregulation of cytokinins allowing morphogenesis and differentiation. Early developmental stages are characterised by cell division, whereas later stages show a rapid transition to growth caused by cell expansion. There seems to be a compensation mechanism at the organ size level allowing increased cell expansion when cell division is compromised and vice versa, giving as a result lower organ size variation. A given species may develop lateral organs differing in size and shape during development, a phenomenon called heteroblasty. A coordination between a basic growth programme and the timing of organ formation seems to be responsible for this phenomenon. Plant organ size is the result of an underlying genetic programme and the interaction with environmental conditions that fine tune the final size of an organ.

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Role of an Atypical E2F Transcription Factor in the Control of Arabidopsis Cell Growth and Differentiation

Cited by 90 publications

References 61 publications

E2F RegulatesFASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status

E2F RegulatesFASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

Control of Plant Organ Size

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