Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Abstract

ObjectivesCaffeic acids are known to have anti-oxidant, anti-inflammatory, immunomodulatory, and tissue reparative effects. The purposes of this study were to determine the effect of caffeic acid on transforming growth factor (TGF) β1-induced myofibroblast differentiation and collagen production, and to determine whether caffeic acid is involved in the antioxidant effect in nasal polyp-derived fibroblasts (NPDFs).MethodsNPDFs were pretreated with caffeic acid (1-10 µM) for 2 hours and stimulated with TGF-β1 (5 ng/mL) for 24 hours. The expression of α-smooth muscle actin (SMA), collagen types I and III, and Nox4 mRNA was determined by a reverse transcription-polymerase chain reaction, and the expression of α-SMA protein was determined by actin ned by immunofluorescence microscopy. The amount of total soluble collagen production was analyzed by the Sircol collagen dye-binding assay. The reactive oxygen species (ROS) generated by NPDFs were determined using 2',7'-dichlorfluorescein-diacetate. siNox4 was used to determine the effect of Nox4.ResultsThe expression of α-SMA and production of collagen were significantly increased following TGF-β1 treatment. In contrast, the level of expression of α-SMA and the level of production of collagen were decreased by pretreatment with caffeic acid. The activation of Nox4 and the subsequent production of ROS were also reduced by pretreatment with caffeic acid. The expression of α-SMA was prevented by inhibition of ROS generation with siNox4.ConclusionCaffeic acid may inhibit TGF-β1-induced differentiation of fibroblasts into myofibroblasts and collagen production by regulating ROS.

Vitamin D attenuates myofibroblast differentiation and extracellular matrix accumulation in nasal polyp-derived fibroblasts through smad2/3 signaling pathway

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Periostin as a marker of mucosal remodelling in chronic rhinosinusitis

“…The role of eosinophil secreted profibrotic cytokines like TGF-β and the deposition of collagen have been reported (16,(19)(20)(21) . Van Bruaene et al have reported early collagen deposition in sinus disease even before the advent of inflammation (44) .…”

Caffeic Acid Inhibits UVB‐induced Inflammation and Photocarcinogenesis Through Activation of Peroxisome Proliferator‐activated Receptor‐γ in Mouse Skin

“…CA downregulates the expression of VEGF, TGF‐ β 1 and iNOS in the UVB‐irradiated mice skin. Previously, CA has been reported to inhibit VEGF, TGF‐ β 1 and iNOS expressions in different carcinoma models . In this study, caffeic acid topical (CAT) treatment showed better photochemoprevention than caffeic acid intraperitoneal (CAIP) treatment.…”