Role of Cyclic AMP in Hydrogen Peroxide-Induced Potentiation of Sympathetic Neurotransmission in the Bovine Iris

Opere, Catherine A.; Ohia, Sunny E.

doi:10.1089/jop.1997.13.261

Cited by 9 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The methodology used for the studies on [ 3 H]NE release was similar to that described by Opere and Ohia [25] with minor modifications. Briefly, isolated porcine iris-ciliary bodies (ICBs) were incubated in fresh Krebs solution containing 2.5 lCi/ml [ 3 H]NE at 37°C for 1 h. After incubation, tissues were rinsed twice with non-radioactive buffer for 5 min, mounted between nylon mesh cloths and placed in thermostatically controlled Superfusion Chambers.…”

Section: Studies On [ 3 H]norepinephrine Releasementioning

confidence: 99%

Effect of Hydrogen Sulfide on Sympathetic Neurotransmission and Catecholamine Levels in Isolated Porcine Iris-Ciliary Body

et al. 2008

View full text Add to dashboard Cite

In the present study, we investigated the pharmacological action of hydrogen sulfide (H2S, using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on sympathetic neurotransmission from isolated, superfused porcine iris-ciliary bodies. We also examined the effect of H2S on norepinephrine (NE), dopamine and epinephrine concentrations in isolated porcine anterior uvea. Release of [3H]NE was triggered by electrical field stimulation and basal catecholamine concentrations was measured by high performance liquid chromatography (HPLC). Both NaHS and Na2S caused a concentration-dependent inhibition of electrically evoked [3H]NE release from porcine iris-ciliary body without affecting basal [3H]NE efflux. The inhibitory action of H2S donors on NE release was attenuated by aminooxyacetic acid (AOA) and propargyglycine (PAG), inhibitors of cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE), respectively. With the exception of dopamine, NaHS caused a concentration-dependent reduction in endogenous NE and epinephrine concentrations in isolated iris-ciliary bodies. We conclude that H2S can inhibit sympathetic neurotransmission from isolated porcine anterior uvea, an effect that is dependent, at least in part, on intramural biosynthesis of this gas. Furthermore, the observed action of H2S donors on sympathetic transmission may be due to a direct action of this gas on neurotransmitter pools.

show abstract

Section: Studies On [ 3 H]norepinephrine Releasementioning

confidence: 99%

Effect of Hydrogen Sulfide on Sympathetic Neurotransmission and Catecholamine Levels in Isolated Porcine Iris-Ciliary Body

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Although the mechanism of action is not clear, the authors suggest that intracellular cysteine levels are enhanced indirectly to form GSH by H 2 S stimulation of glutamate/cystine antiporters (Sparatore et al, 2009; Perrino et al, 2009; Osborne et al, 2010). Moreover, as mentioned above, a study by Opere and Ohia (1997) had demonstrated the inhibitory action of H 2 S donors on glutamatergic transmission in mammalian retinae. So, it is plausible that H 2 S donors can render there cytoprotective effects by upregulating system x − C transporter in the retina and thereby increasing the production of glutathione.…”

Section: Regulation Of Transporters By H2s In the Eyementioning

confidence: 84%

Hydrogen sulfide: role in ion channel and transporter modulation in the eye

Njie‐Mbye¹,

Opere²,

Chitnis³

et al. 2012

Front. Physio.

View full text Add to dashboard Cite

Hydrogen sulfide (H2S), a colorless gas with a characteristic smell of rotten eggs, has been portrayed for decades as a toxic environmental pollutant. Since evidence of its basal production in mammalian tissues a decade ago, H2S has attracted substantial interest as a potential inorganic gaseous mediator with biological importance in cellular functions. Current research suggests that, next to its counterparts nitric oxide and carbon monoxide, H2S is an important multifunctional signaling molecule with pivotal regulatory roles in various physiological and pathophysiological processes as diverse as learning and memory, modulation of synaptic activities, cell survival, inflammation, and maintenance of vascular tone in the central nervous and cardiovascular systems. In contrast, there are few reports of a regulatory role of H2S in the eye. Accumulating reports on the pharmacological role of H2S in ocular tissues indicate the existence of a functional trans-sulfuration pathway and a potential physiological role for H2S as a gaseous neuromodulator in the eye. Thus, understanding the role of H2S in vision-related processes is imperative to our expanding knowledge of this molecule as a gaseous mediator in ocular tissues. This review aims to provide a comprehensive and current understanding of the potential role of H2S as a signaling molecule in the eye. This objective is achieved by discussing the involvement of H2S in the regulation of (1) ion channels such as calcium (L-type, T-type, and intracellular stores), potassium (KATP and small conductance channels) and chloride channels, (2) glutamate transporters such as EAAT1/GLAST and the L-cystine/glutamate antiporter. The role of H2S as an important mediator in cellular functions and physiological processes that are triggered by its interaction with ion channels/transporters in the eye will also be discussed.

show abstract

Rp-cAMPS has ho effect on adenosine A 1 receptors in guinea-pig cardiomyocytes

Adamson

Harding

2000

Basic Research in Cardiology

View full text Add to dashboard Cite

Rp-cAMPS, a protein kinase A inhibitor, is used in the investigation of the cAMP-dependent systems. A report by Musgrave et al. has suggested that Rp-cAMPS may also act on adenosine receptors. To determine whether this occurs in guinea-pig ventricular myocytes, Rp-cAMPS was applied in the presence and absence of DCPCX, an adenosine A receptor antagonist. The isoprenaline-induced response was significantly decreased by Rp-cAMPS and the effect was not altered by the presence of DCPCX. Therefore Rp-cAMPS has no effect on cell contraction via adenosine A1 receptors and can reliably be used to investigate cyclic AMP-dependent systems in isolated cardiac myocytes.

show abstract

Role of Cyclic AMP in Hydrogen Peroxide-Induced Potentiation of Sympathetic Neurotransmission in the Bovine Iris

Cited by 9 publications

References 18 publications

Effect of Hydrogen Sulfide on Sympathetic Neurotransmission and Catecholamine Levels in Isolated Porcine Iris-Ciliary Body

Effect of Hydrogen Sulfide on Sympathetic Neurotransmission and Catecholamine Levels in Isolated Porcine Iris-Ciliary Body

Hydrogen sulfide: role in ion channel and transporter modulation in the eye

Rp-cAMPS has ho effect on adenosine A 1 receptors in guinea-pig cardiomyocytes

Contact Info

Product

Resources

About