Role of IL-4 in aversion induced by food allergy in mice

Dourado, Luana Pereira Antunes; Saldanha, Janaína Cláudia da Silva; Gargiulo, Daniela Longo; Noviello, Maria de Lourdes Meirelles; Brant, Cláudia Caldeira; Reis, Maria Letícia Costa; Souza, Raphaela Mendes Fernandes de; Faria, Ana Maria Caetano; Souza, Danielle da Glória de; Cara, Denise Carmona

doi:10.1016/j.cellimm.2009.12.010

Cited by 28 publications

(23 citation statements)

References 21 publications

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“…It was shown that this process happens through IgE-mediated mast cell degranulation Prolonged antigen ingestion and food allergy with consequent 5-HT3 signalling [28]. It has also been shown that this aversion is immune-based [29] and is dependent upon both IgE [20] and IL-4 [18]. Therefore, these data support the breakdown of antigen aversion that happens in sensitized mice after 14 days of prolonged OVA ingestion when the specific IgE levels are decreased significantly, while IgG1 is kept at the same level of sensitized mice that were only challenged orally for 7 days.…”

Section: Discussionmentioning

confidence: 99%

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Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

Batista¹,

Pereira²,

Noviello³

et al. 2014

Clinical and Experimental Immunology

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SummaryThe prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of oral challenge, sensitized mice showed an anti-OVA IgE level similar to the mice that were only sensitized, but the specific IgG1 did not change. With this prolonged ingestion of OVA, sensitized mice were protected from OVA-induced anaphylaxis when the antigen was given systemically at a dose of 2 mg/animal. Moreover, various parameters analysed were significantly ameliorated, including adipose tissue inflammation, body and adipose tissue loss, as well as serum levels of adipokines and triglycerides. Therefore, our data suggest that prolonged ingestion of OVA by sensitized mice results in an improvement of the metabolic consequences caused by experimental food allergy.

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Section: Discussionmentioning

confidence: 99%

“…The enzyme-linked immunosorbent assay (ELISA) test for anti-OVA IgE was performed using plates coated with rat anti-mouse IgE, serum and biotinylated OVA, as described previously [18]. The reaction was developed with streptavidin-peroxidase conjugate (ExtraAvidin; Sigma), o phenylene-diamine and H2O2.…”

Section: Evaluation Of Serum Ova-specific Ige and Igg1mentioning

confidence: 99%

Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

Batista¹,

Pereira²,

Noviello³

et al. 2014

Clinical and Experimental Immunology

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“…IL-4 is essential in Th2-type responses during FA [23,24]. It prompts B-cell activation and interacts with antigen-specific B cells, leading to isotype switching to generate food antigen-specific IgE [25].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, IL-4 was shown to enhance the release of histamine as well as leukotrienes from mast cells triggered by IgE receptor crosslinking [27]. IL-4 deficiency was shown to limit FA development [24]. Inversely, a gain-of-function mutation in the IL-4 receptor α-chain was shown to increase susceptibility to anaphylaxis [28].…”

Section: Discussionmentioning

confidence: 99%

Eppikajutsuto Protects against Food Allergy Induced by Ovalbumin in a Murine Model

Matsui

Yamashita

Mori

et al. 2017

Int Arch Allergy Immunol

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Background: Currently, there are no efficient medications available for the prevention and treatment of food allergy (FA). Herbal medicines, including traditional Japanese Kampo medicines (TJKMs), are promising therapeutic drugs. Methods: We screened 18 TJKMs for treatment of FA symptoms in a mouse FA model induced by ovalbumin (OVA). BALB/c mice were sensitized intraperitoneally by an OVA/aluminum hydroxide gel mixture followed by 4 booster doses of oral OVA and FA symptom induction by 50 mg of OVA. TJKMs were orally administered for 28 days from the day of sensitization to the day before FA symptom induction. Evaluated FA symptoms included a decrease in body temperature and allergic diarrhea. Allergic sensitization was determined by plasma OVA-specific IgE levels. Cytokine mRNA levels in mesenteric lymph nodes, plasma mouse mast cell protease-1, and the number of mast cells in the small and large intestines were analyzed. Additionally, the therapeutic effect of the TJKM eppikajutsuto (EJT) on mast cell degranulation was determined in active anaphylaxis and passive cutaneous anaphylaxis models. Results: EJT effectively prevented FA symptoms. Although OVA-specific IgE levels and the intestinal mast cell numbers were not different between the EJT-treated and untreated FA mice, plasma mMcpt1 and IL-4 levels were lower in EJT-treated FA mice than untreated FA mice. EJT could alleviate symptoms in both active and passive anaphylaxis models. Conclusion: EJT prevented OVA-induced FA symptoms in a mouse model, suggesting that EJT might exert its therapeutic activity via IL-4 suppression and the inhibition of mucosal mast cell degranulation.

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“…However, how BZS exerts its anti-abortive function is as yet unknown. Many studies have demonstrated that cytokines play an important role during pregnancy, by creating microenvironments that modulate maternal immune responses (Jacques and Qureshi 1998;Jiang and Vacchio 1998;Paradisi et al 2003;Chaouat et al 2009;Dourado et al 2010). Therefore, the interrelation between BZS and changes in the maternal immune response may be significant for the mechanism of action of BZS in preventing abortion.…”

Section: Discussionmentioning

confidence: 99%

Effects of Bai zhu san (Atractylodis macrocephalae) decoction on cellular immunity and Th1/Th2 cytokine ratio in a Mifepristone-induced murine abortion model

Geng¹,

Yuan²,

Wang³

et al. 2014

Vet. Med. - Czech

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Chinese herbal medicine has been used as an anti-abortive treatment in farm animals for thousands of years. To investigate the anti-abortive functions and mechanisms of Bai zhu san (BZS) on mifepristone (RU-486)-induced abortion, pregnant mice were allocated to different groups as follows: control group receiving neither RU-486 nor BZS; mice receiving RU-486 only; mice treated with both RU-486 and BZS. The results showed that the concentrations of IL-10 and IL-4 in uterine lysates were significantly higher in mice of the BZS + RU-486 group compared with the mice of the RU-486 group. The IL-10 and IL-4 levels in serum were several folds higher than that in uterus lysates. The IFN-γ concentrations in uterine lysate decreased significantly in mice of the BZS + RU-486 group vs. the RU-486 group. There were no significant differences in IL-2 concentrations between the mice of BZS + RU-486 or RU-486 groups and controls in the uterus and serum. The numbers of CD4 + , CD8 + T lymphocytes or macrophages in the uterus decreased in the BZS + RU-486 group compared with the RU-486 group. These results indicate that the Chinese herbal formula Bai zhu san inhibits RU-486-induced abortion and modulates the Th1/Th2 cytokine balance at the maternal-foetal interface.

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Role of IL-4 in aversion induced by food allergy in mice

Cited by 28 publications

References 21 publications

Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

Eppikajutsuto Protects against Food Allergy Induced by Ovalbumin in a Murine Model

Effects of Bai zhu san (Atractylodis macrocephalae) decoction on cellular immunity and Th1/Th2 cytokine ratio in a Mifepristone-induced murine abortion model

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