Role of interferon regulatory factor 7 in corneal endothelial cells after HSV-1 infection

Ohtani, Fumie; Miyazaki, Dai; Shimizu, Yumiko; Haruki, Tomoko; Yamagami, Satoru; Inoue, Yoshitsugu

doi:10.1038/s41598-021-95823-9

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…We found several TFs, such as Irf7, Creb5, Elk3, Fosl1 and Klf7 , appeared in the top up-regulated genes in four types cells. IRF7 is a member of the interferon regulatory factors (IRFS) family and participates in type-I interference (IFN) signaling, and plays a role in antigen presentation function of human corneal endothelial cells [ 62 , 63 ]. The latest research showed that FOSL1 as the human LSC-specific TFs that determined the LSC fate and was important in epidermal cells [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing

Sun,

Zhang,

Chen

et al. 2024

Stem Cell Res Ther

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Background Dysfunction or deficiency of corneal epithelium results in vision impairment or blindness in severe cases. The rapid and effective regeneration of corneal epithelial cells relies on the limbal stem cells (LSCs). However, the molecular and functional responses of LSCs and their niche cells to injury remain elusive. Methods Single-cell RNA sequencing was performed on corneal tissues from normal mice and corneal epithelium defect models. Bioinformatics analysis was performed to confirm the distinct characteristics and cell fates of LSCs. Knockdown of Creb5 and OSM treatment experiment were performed to determine their roles of in corneal epithelial wound healing. Results Our data defined the molecular signatures of LSCs and reconstructed the pseudotime trajectory of corneal epithelial cells. Gene network analyses characterized transcriptional landmarks that potentially regulate LSC dynamics, and identified a transcription factor Creb5, that was expressed in LSCs and significantly upregulated after injury. Loss-of-function experiments revealed that silencing Creb5 delayed the corneal epithelial healing and LSC mobilization. Through cell–cell communication analysis, we identified 609 candidate regeneration-associated ligand-receptor interaction pairs between LSCs and distinct niche cells, and discovered a unique subset of Arg1+ macrophages infiltrated after injury, which were present as the source of Oncostatin M (OSM), an IL-6 family cytokine, that were demonstrated to effectively accelerate the corneal epithelial wound healing. Conclusions This research provides a valuable single-cell resource and reference for the discovery of mechanisms and potential clinical interventions aimed at ocular surface reconstruction.

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Section: Resultsmentioning

confidence: 99%

Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing

Sun,

Zhang,

Chen

et al. 2024

Stem Cell Res Ther

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“…Dhanushkodi et al [ 221 ] also discovered IFN-γ-producing PLZFloRORγtlo as the most prevalent Invariant Natural Killer 1 cells in HSK-infected corneas[ 221 ]. At the level of the infected endothelial layer, IFN regulatory factor-7 has been shown to upregulate acquired immunity and mediate the major histocompatibility complex resulting from HSV infection[ 222 ].…”

Section: Immune Responses In Herpes Simplex Keratitis: Implications F...mentioning

confidence: 99%

Herpes simplex keratitis: A brief clinical overview

Musa,

Enaholo,

Aluyi-Osa

et al. 2024

World J Virol

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The aim of our minireview is to provide a brief overview of the diagnosis, clinical aspects, treatment options, management, and current literature available regarding herpes simplex keratitis (HSK). This type of corneal viral infection is caused by the herpes simplex virus (HSV), which can affect several tissues, including the cornea. One significant aspect of HSK is its potential to cause recurrent episodes of inflammation and damage to the cornea. After the initial infection, the HSV can establish a latent infection in the trigeminal ganglion, a nerve cluster near the eye. The virus may remain dormant for extended periods. Periodic reactivation of the virus can occur, leading to recurrent episodes of HSK. Factors triggering reactivation include stress, illness, immunosuppression, or trauma. Recurrent episodes can manifest in different clinical patterns, ranging from mild epithelial involvement to more severe stromal or endothelial disease. The severity and frequency of recurrences vary among individuals. Severe cases of HSK, especially those involving the stroma and leading to scarring, can result in vision impairment or even blindness in extreme cases. The cornea's clarity is crucial for good vision, and scarring can compromise this, potentially leading to visual impairment. The management of HSK involves not only treating acute episodes but also implementing long-term strategies to prevent recurrences and attempt repairs of corneal nerve endings via neurotization. Antiviral medications, such as oral Acyclovir or topical Ganciclovir, may be prescribed for prophylaxis. The immune response to the virus can contribute to corneal damage. Inflammation, caused by the body's attempt to control the infection, may inadvertently harm the corneal tissues. Clinicians should be informed about triggers and advised on measures to minimize the risk of reactivation. In summary, the recurrent nature of HSK underscores the importance of both acute and long-term management strategies to preserve corneal health and maintain optimal visual function.

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Viral Infection and Ischemic Stroke: Emerging Trends and Mechanistic Insights

Clarke,

Falcione,

Boghozian

et al. 2024

JAHA

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Population studies have suggested that viral infections may be contributing to risk of ischemic stroke, although the mechanisms for this are unclear. In this review, we examine the epidemiological evidence supporting the involvement of viral diseases, including influenza, COVID‐19, chronic herpesvirus infections, and hepatitis C in current trends of stroke incidence. To support these associations, we highlight the virus–host interactions that are critical in the context of stroke, including direct effects of acute and persistent viral infections on vascular function, inflammation, and thrombosis. Additionally, we evaluate the systemic changes that occur during viral infection that can predispose individuals to ischemic stroke, including alterations in blood pressure regulation, coagulation, and lipid metabolism. Our review emphasizes the need to further elucidate precise mechanisms involved in viral infections and stroke risk. Future research will inform the development of targeted interventions for stroke prevention in the context of viral diseases.

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Role of interferon regulatory factor 7 in corneal endothelial cells after HSV-1 infection

Cited by 4 publications

References 31 publications

Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing

Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing

Herpes simplex keratitis: A brief clinical overview

Viral Infection and Ischemic Stroke: Emerging Trends and Mechanistic Insights

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