Role of lncRNAs in Cellular Aging

Degirmenci, Ufuk; Sun, Li

doi:10.3389/fendo.2016.00151

Cited by 40 publications

(24 citation statements)

References 119 publications

(104 reference statements)

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“…However, no previous studies have focused on the role of lncRNAs in early AMD. Considering the extensive effect of lncRNAs on physiological function, 11 it is reasonable to think that lncRNAs can significantly influence the development and progression of early AMD. In the present study, we identified a set of lncRNAs that were differentially expressed in early AMD, confirmed the expression patterns of selected lncRNAs in an in vitro model, and performed a preliminary investigation into the biological effects of dysregulated lncRNA on AMD development.…”

mentioning

confidence: 99%

Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

Zhu

Xing

et al. 2017

IJN

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Background Age-related macular degeneration (AMD) is one of the most common causes of adult blindness in developed countries. However, the role of long noncoding RNAs (lncRNAs) in the development and progression of early AMD is unclear. Methods We established the lncRNA profile of early AMD by reannotation of microarrays from the gene expression omnibus database. Quantitative real-time polymerase chain reaction was used to determine the expression of selected lncRNAs. Results The expression profiles of 9 cases of AMD and 7 controls were studied. A total of 266 differentially expressed genes (DEGs) were detected (94 upregulated and 172 downregulated). Among all the DEGs, 64 were lncRNAs. Advanced bioinformatics analyses demonstrated that differentially expressed lncRNAs could play significant roles in visual perception, sensory perception of light stimulus, and cognition. The pathway analyses showed that the two most significantly influenced Kyoto Encyclopedia of Genes and Genomes pathways were those of phototransduction and purine metabolism. By the analyses of the key lncRNAs, it was found that RP11-234O6.2 was downregulated in the aging retinal pigment epithelium (RPE) cellular model. Exogenous RP11-234O6.2 treatment led to increased cell viability and improved apoptosis but it did not affect the cell migration ability of aging RPE cells. Conclusion This study indicated that lncRNAs are differentially expressed in early AMD and may produce important regulative effects. An lncRNA, RP11-234O6.2, might be involved in the biological regulation of early AMD and have therapeutic potential.

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confidence: 99%

Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

Zhu

Xing

et al. 2017

IJN

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“…Hence, any cause that affects the proliferation capacity of spermatogonia can lead to spermatogenesis process disorders. It has been proved that almost all cellular processes are modulated by lncRNAs, and the imbalance of these noncoding molecules appear to be associated with the development of a variety of diseases (Degirmenci & Lei, ). A study of Lu et al confirmed that downregulation of miR‐320a/383 sponge‐like lncRNA NLC1‐C has correlation with male infertility and accelerates the proliferation of testicular embryonal carcinoma cells (Lu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA HOTTIP is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation

Zhou

Zhang

et al. 2019

Molec Gen & Gen Med

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Background It has been proposed that lncRNAs, widely transcribed from genomes, play pivotal regulatory roles in a variety of biological processes, but their function in regulating spermatogenesis in human males is rarely reported. Methods QRT‐PCR was adopted to detect HOTTIP expression level in testicular tissues from hypospermatogenesis (Hypo) patients or controls. The proliferation levels of NT2 and 293T were measured via CCK‐8 and EdU detection. Meanwhile, luciferase reporter gene assay and bioinformatics analysis were carried out to identify a target of HOTTIP. Additionally, the underlying mechanism of HOTTIP’s function was investigated using western blotting and RIP analysis. Results The research results manifested that the expression of HOTTIP in testicular tissues from Hypo patients was prominently reduced in comparison with that in control testicular tissues. Interestingly, it was noted that HOTTIP exhibited a high expression in testicular embryonal carcinoma cell line NT2 compared with that in normal control cell line 293T. It was denoted in cell function evaluation that cell proliferation was impeded by downregulated HOTTIP but evidently stimulated by overexpressed HOTTIP. Moreover, HOTTIP was capable of positively modulating HOXA13 expression via the competitive binding to miR‐128‐3p. Conclusion Therefore, HOTTIP acting as ceRNAs to promote testicular embryonal carcinoma cell proliferation.

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“…LncRNAs constitute non-coding RNAs over 200 nt long, which regulate gene expression patterns at the transcriptional, post-transcriptional and post-translational levels. LncRNAs display tissue specific gene expression 64,65 and are thought to have direct roles in the aging process. Putative roles for other lncRNAs have been identified in conditions such as heart failure, cardiac autophagy, hypertension, acute kidney injury, glomerular diseases, acute allograft rejection and renal cell carcinoma.…”

Section: Long Non-coding Rnas (Lncrnas)mentioning

confidence: 99%

The role of epigenetics in renal ageing

Shiels

McGuinness

Eriksson³

et al. 2017

Nat Rev Nephrol

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An ability to separate natural ageing processes from processes specific to morbidities is required to understand the heterogeneity of age-related organ dysfunction. Mechanistic insight into how epigenetic factors regulate ageing throughout the life course, linked to a decline in renal function with ageing, is already proving to be of value in the analyses of clinical and epidemiological cohorts. Noncoding RNAs provide epigenetic regulatory circuits within the kidney, which reciprocally interact with DNA methylation processes, histone modification and chromatin. These interactions have been demonstrated to reflect the biological age and function of renal allografts. Epigenetic factors control gene expression and activity in response to environmental perturbations. They also have roles in highly conserved signalling pathways that modulate ageing, including the mTOR and insulin/insulin-like growth factor signalling pathways, and regulation of sirtuin activity. Nutrition, the gut microbiota, inflammation and environmental factors, including psychosocial and lifestyle stresses, provide potential mechanistic links between the epigenetic landscape of ageing and renal dysfunction. Approaches to modify the renal epigenome via nutritional intervention, targeting the methylome or targeting chromatin seem eminently feasible, although caution is merited owing to the potential for intergenerational and transgenerational effects.

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Role of lncRNAs in Cellular Aging

Cited by 40 publications

References 119 publications

Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

Long noncoding RNA HOTTIP is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation

The role of epigenetics in renal ageing

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