Role of p38 MAPK Signalling in Testis Development and Male Fertility

Luo, Dandan; He, Zhao; Yu, Chunxiao; Guan, Qingbo

doi:10.1155/2022/6891897

Cited by 33 publications

(20 citation statements)

References 145 publications

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“…Previous studies have demonstrated that the MAPK signaling pathway participated in oxidative stress 23 and that H 2 O 2 and t-BHP could trigger apoptosis by phosphorylating ERK, JNK, and p38 in testis. As shown in Figure 7, compared with the AN group, the phosphorylation levels of JNK and p38 were significantly higher in the DN group although there was no significant change in the phosphorylated ERK.…”

Section: Alteration Of Mitogen-activated Protein Kinases (Mapks) and ...mentioning

confidence: 99%

N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice

Zhao

Wang

Yang

et al. 2023

J. Agric. Food Chem.

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Homeostasis of reactive oxygen species is required to maintain sperm maturation and capacitation. Docosahexaenoic acid (DHA) is accumulated in testicles and spermatozoa and has the ability to manipulate the redox status. The effects of dietary n-3 polyunsaturated fatty acid (n-3 PUFA) deficiency from early life to adulthood on the physiological and functional properties of males under the redox imbalance of testicular tissue deserve attention. The consecutive injection of hydrogen peroxide (H 2 O 2 ) and tertbutyl hydroperoxide (t-BHP) for 15 days to induce oxidative stress in testicular tissue was used to elucidate the consequences of testicular n-3 PUFA deficiency. The results indicated that reactive oxygen species treatment in adult male mice with DHA deficiency in the testis could reduce spermatogenesis and disrupt sex hormone production, as well as trigger testicular lipid peroxidation and tissue damage. N-3 PUFA deficiency from early life to adulthood resulted in higher susceptibility to testicular dysfunction in the germinal function of supplying germ cells and the endocrine role of secreting hormones through the mechanism of aggravating mitochondria-mediated apoptosis and destruction of blood testicular barrier under oxidative stress, which might provide a basis for humans to reduce susceptibility to chronic disease and maintain reproductive health in adulthood through dietary interventions of n-3 PUFAs.

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Section: Alteration Of Mitogen-activated Protein Kinases (Mapks) and ...mentioning

confidence: 99%

N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice

Zhao

Wang

Yang

et al. 2023

J. Agric. Food Chem.

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“…The p38 MAPKs belong to the MAPK family, playing an essential role in cellular responses including cell proliferation, differentiation, apoptosis, inflammation, and cellular stress responses. 40 Thus, it is important to identify and characterize insect p38 MAPKs to provide a theoretical basis for explaining the Bt insecticidal mechanism. In this study, one p38 MAPK gene was identified from the S. exigua transcriptome and genome database (Figure 1).…”

Section: ■ Discussionmentioning

confidence: 99%

Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

Ji,

Gao,

Zhao

et al. 2024

J. Agric. Food Chem.

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The p38 mitogen-activated protein kinases (MAPKs) are associated with insect immunity, tissue repair, and the insecticidal activity of Bacillus thuringiensis (Bt). Here, a p38 MAPK family gene (Sep38β) was identified from Spodoptera exigua. Among the developmental stages, the transcription level of Sep38β was the highest in egg, followed by that in prepupa and pupa. Sep38β expression peaked in Malpighian tubules and the hemolymph of fifth instar larvae. Knockdown of Sep38β or injection of SB203580 (a p38 MAPK inhibitor) significantly downregulated the SeDUOX expression and reactive oxygen species (ROS) level in the midgut, accounting for deterioration of the midgut to scavenge pathogens and enhancement of Bt insecticidal activity. In conclusion, all the results demonstrate that Sep38β regulates the immune-related ROS level in the insect midgut, which suppresses the insecticidal activity of Bt against S. exigua by 17−22%. Our study highlights that Sep38β is essential for insect immunity and the insecticidal activity of Bt to S. exigua and is a potential target for pest control.

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“…Gossypol also impaired mouse testicular development through mitogen-activated protein kinase pathway inactivation [ 29 ]. Mitogen-activated protein kinase pathway was crucial signaling during spermatogenesis integrating its role in cell proliferation, differentiation, apoptosis, and cellular stress response [ 39 , 40 ]. Gossypol also interferes with the expression of several genes associated with testicular development, such as cell proliferation, spermatogenesis, and maturation of germ cells [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The reversible effects of gossypol toxicity on male pigeons' reproductive performance

et al. 2022

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Background and Aim: Gossypol, a cotton seed derivative, is well known for its reversible antifertility in male reproduction across species. Its antifertility and reversibility effects on male reproductive function vary among species in dose-and time-dependent manners. In this study, the antifertility potential of gossypol in pigeons was evaluated for the first time to determine whether it might be used as a dietary supplement for pigeon population control. Materials and Methods: Male pigeons were assigned into three experimental groups: The gossypol-treated group (n = 12), the sham control group (n = 6), and the negative control group (n = 6). There were two experimental periods: A gossypol-feeding period of 28 days and a gossypol-free period of 28 days. During the gossypol-feeding period, birds in the gossypol-treated group were fed 4 mg of gossypol extract per day. Birds in the sham control group were fed 0.5 mL of mixed ethanol and sunflower oil, while those in the negative control group were fed 0.5 mL of phosphate buffer saline. After the gossypol-feeding phase was completed, all remaining pigeons in all groups continued to receive their regular diet for an additional 28 days (gossypol-free phase). The body weight and semen quality of the birds in the experimental groups were compared to evaluate gossypol's antifertility effect. Results: In the gossypol-treated group as compared to the control groups, the percentages of sperm motility and viability were significantly lower at 21 days, and the percentage of normal sperm morphology was significantly lower at 28 days during the gossypol-feeding period. After gossypol withdrawal, these antifertility effects were resumed and reached a comparable semen quality to the control groups within 14 days. Conclusion: Gossypol supplementation (4 mg/day for 28 days) could lower male pigeons' reproductive performance in terms of sperm motility, viability, and sperm morphology. Such infertility was, however, reversible within 14 days after gossypol withdrawal without any side effects on the pigeons, suggesting its application as a safe contraceptive feeding for male pigeons.

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Role of p38 MAPK Signalling in Testis Development and Male Fertility

Cited by 33 publications

References 145 publications

N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice

N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice

Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

The reversible effects of gossypol toxicity on male pigeons' reproductive performance

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