Role of protein kinase C-mediated pathway in the pathogenesis of coronary artery spasm in a swine model.

Ito, Akira; Shimokawa, Hiroaki; Nakaike, Ryuichi; Fukai, Tohru; Sakata, Makoto; Takayanagi, T; Egashira, Kensuke; Takeshita, Akira

doi:10.1161/01.cir.90.5.2425

Cited by 44 publications

(50 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cineangiograms were projected on a screen using a cineprojector (ELK-35CB; Nishimoto Sangyo Inc., Osaka, Japan), and an end-diastolic frame was selected and printed. The coronary luminal diameters were measured with a caliper (4)(5)(6)16). Using this technique, excellent correlations between repeated measurements ( r ϭ 0.99) as well as between different observers ( r ϭ 0.98) were confirmed in the range of the coronary diameter from 0.98 mm to 5.58 mm (16).…”

Section: Methodsmentioning

confidence: 98%

“…A preshaped Judkins catheter was inserted into the right or left femoral artery, and coronary arteriography in a left anterior oblique view was performed. Electrocardiograms (I, II, III, V1, and V6 leads) and blood pressure were continuously recorded throughout the experiments (4)(5)(6)16).…”

Section: Methodsmentioning

confidence: 99%

“…), and prostaglandin F2 ␣ (5 and 50 g/kg i.c.) (16). Coronary arteriography was performed 2 min after the intracoronary administration of serotonin, 1 min after that of histamine, and 5 min after that of PGF2 ␣ .…”

Section: Methodsmentioning

confidence: 99%

“…Coronary arteriography was performed 2 min after the intracoronary administration of serotonin, 1 min after that of histamine, and 5 min after that of PGF2 ␣ . Ischemic electrocardiogram (ECG) 1 changes were defined as a Ͼ 1-mm ST segment elevation or depression in more than two leads (4)(5)(6)16). Coronary diameter measurement.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Chronic treatment with interleukin-1 beta induces coronary intimal lesions and vasospastic responses in pigs in vivo. The role of platelet-derived growth factor.

et al. 1996

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Chronic treatment with interleukin-1 beta induces coronary intimal lesions and vasospastic responses in pigs in vivo. The role of platelet-derived growth factor.

et al. 1996

Self Cite

View full text Add to dashboard Cite

“…Ito et al, 11 Katsumata et al, 12 and Kandabashi et al 13 have investigated the intracellular mechanism for spasm using swine coronary spasm models. They indicate that the protein kinase C-mediated pathway and enhanced myosin light chain phosphorylation play an important role in the enhanced constrictor response of the coronary artery smooth muscle to autocoids such as serotonin and histamine.…”

mentioning

confidence: 99%

Enhanced Activity of Variant Phospholipase C-δ1 Protein (R257H) Detected in Patients With Coronary Artery Spasm

et al. 2002

View full text Add to dashboard Cite

Background-We recently demonstrated that phospholipase C (PLC)-␦1 activity in cultured skin fibroblasts obtained from patients with coronary spastic angina (CSA) is enhanced. We tested the hypothesis that structural abnormality in PLC-␦1 isoform is a cause of the enhanced activity. Methods and Results-Sequence analysis of the cDNA coding for PLC-␦1 obtained from fibroblasts revealed that one conversion of guanine to adenine (A) was present at nucleotide position 864 in one CSA patient, resulting in the amino acid replacement of arginine 257 by histidine (R257H). The incidence of 864A/A in genomic DNA, analyzed by single-strand conformation polymorphism, was greater in patients with CSA than in male control subjects (6 of 57 patients with CSA versus 1 of 62 control subjects, PϽ0.05). The activity of the variant PLC-␦1 protein under free calcium concentration between 10 Ϫ8 and 10 Ϫ7 mol/L was 2-fold higher than that of the wild-type protein. Baseline intracellular calcium concentration ([Ca 2ϩ ] i ) in human embryonic kidney 293 cells transfected with the variant PLC-␦1 was higher than that in cells with the wild type. The peak increase in [Ca 2ϩ ] i in response to acetylcholine at 10 Ϫ6 and 10 Ϫ5 mol/L was greater in the cells with the variant PLC-␦1 than in those with the wild type. Conclusions-These findings indicate that the R257H variant in the PLC-␦1 gene detected in patients with CSA is associated with enhancement of enzyme activity, and they describe a novel mechanism for the enhanced coronary vasomotility in

show abstract

Increased oxidative stress with elevated serum thioredoxin level in patients with coronary spastic angina

Miwa

Kishimoto

Nakamura

et al. 2003

Clinical Cardiology

View full text Add to dashboard Cite

SummaryBackground: Increased oxidative stress has been implicated in the pathogenesis of coronary vasospasm. Thioredoxin (TRX) is a redox-active protein that is known to be induced by oxidative stress. Hypothesis:The serum TRX level may be high in patients with coronary vasospasm. Methods:The serum TRX level was determined using an enzyme-linked immunosorbent assay in 21 patients with the active stage of coronary spastic angina (CSA), in 18 patients with the inactive stage of CSA (iCSA), in 24 control subjects without coronary artery disease (Control), and in 20 patients with stable effort angina (SEA).Results: Serum TRX levels (mean ± standard deviation ng/ml) were significantly higher in CSA (64 ± 44) than in iCSA (28 ± 26), in Control (34 ± 15), and in SEA (36 ± 16). In contrast, serum ␣-tocopherol levels (mg/g lipids) were significantly lower in CSA (2.8 ± 0.7) than in Control (4.0 ± 1.2) and in SEA (3.2 ± 0.4). Current smoking was significantly more prevalent in CSA (76%) than in any of the other groups. No significant correlation was found between the serum level of TRX and ␣-tocopherol in the study subjects. In nine patients

show abstract

Role of protein kinase C-mediated pathway in the pathogenesis of coronary artery spasm in a swine model.

Cited by 44 publications

References 35 publications

Chronic treatment with interleukin-1 beta induces coronary intimal lesions and vasospastic responses in pigs in vivo. The role of platelet-derived growth factor.

Chronic treatment with interleukin-1 beta induces coronary intimal lesions and vasospastic responses in pigs in vivo. The role of platelet-derived growth factor.

Enhanced Activity of Variant Phospholipase C-δ1 Protein (R257H) Detected in Patients With Coronary Artery Spasm

Increased oxidative stress with elevated serum thioredoxin level in patients with coronary spastic angina

Contact Info

Product

Resources

About