Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection

Zanetti, Simone; Meola, Marco; Bochud, Arlette; Puoti, Alessandro

doi:10.1016/j.ydbio.2011.04.001

Cited by 28 publications

(35 citation statements)

References 63 publications

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“…We reasoned that if MOG proteins down-regulated fem-3 RNA, both proteins should not be found within the same germ cells. MOG proteins are present in the nuclei of many germ cells, including oocytes (Belfiore et al 2004;Kasturi et al 2010;Zanetti et al 2011). We show that FEM-3 labels the spermatogenic lineage.…”

Section: Fem-3 and Mog Expression Are Mutually Exclusivementioning

confidence: 73%

“…Although several trans-acting repressors of fem-3 have been identified, the molecular mechanism of fem-3 repression remains speculative (Zhang et al 1997;Kimble 1999, 2000;Zanetti et al 2011). One study reported that in the absence of larp-1, fem-3(+) mRNA levels are increased (Zanin et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…RNA was extracted and reverse-transcribed as described using either random oligonucleotides or specific antisense primers (Zanetti et al 2011). The 39 untranslated region of fem-3 was amplified by PCR and cloned into pBluescript for the sequencing assay (alleles q23 and q96).…”

Section: Quantification Of Fem-3 Mrnamentioning

confidence: 99%

“…fem-3(q95) was analyzed by Southern blotting or real-time PCR. fem-3(q23) was also detected through digestion with BsaXI; after extensive digestion, products were analyzed by Southern blotting and quantified with an Amersham Biosciences Phosphoimager (Zanetti et al 2011). Quantitative real-time PCR was performed on cDNAs using two FAM-conjugated TaqMan probes that are specific either for the wild-type or the fem-3(q95) mutated fem-3 39 UTR.…”

Section: Quantification Of Fem-3 Mrnamentioning

confidence: 99%

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The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

Zanetti¹,

Grinschgl²,

Meola³

et al. 2012

RNA

Self Cite

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Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 39 UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability.

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Section: Fem-3 and Mog Expression Are Mutually Exclusivementioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Quantification Of Fem-3 Mrnamentioning

confidence: 99%

Section: Quantification Of Fem-3 Mrnamentioning

confidence: 99%

See 2 more Smart Citations

The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

Zanetti¹,

Grinschgl²,

Meola³

et al. 2012

RNA

Self Cite

View full text Add to dashboard Cite

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“…As another example, the cell cycle regulators Cyclin E (CYE-1) and CDK2 (CDK-2) promote the proliferative fate (Fox et al, 2011; Jeong et al, 2011). A number of splicing factors have also been identified as being involved in regulating the proliferation vs. differentiation decision (Belfiore et al, 2004; Mantina et al, 2009; Kerins et al, 2010; Zanetti et al, 2011). Genetically, these splicing factors appear to function largely in the gld-1 pathway, functioning downstream of GLP-1/Notch signaling (Figure 1B).…”

Section: Introductionmentioning

confidence: 99%

TEG‐1 CD2BP2 regulates stem cell proliferation and sex determination in the C. elegans germ line and physically interacts with the UAF‐1 U2AF65 splicing factor

Wang

Wilson-Berry

Schedl

et al. 2012

Developmental Dynamics

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Background For a stem cell population to exist over an extended period, a balance must be maintained between self-renewing (proliferating) and differentiating daughter cells. Within the Caenorhabditis elegans germ line, this balance is controlled by a genetic regulatory pathway, which includes the canonical Notch signaling pathway. Results Genetic screens identified the gene teg-1 as being involved in regulating the proliferation vs. differentiation decision in the C. elegans germ line. Cloning of TEG-1 revealed that it is a homolog of mammalian CD2BP2, which has been implicated in a number of cellular processes, including in U4/U6.U5 tri-snRNP formation in the pre-mRNA splicing reaction. The position of teg-1 in the genetic pathway regulating the proliferation vs. differentiation decision, its single mutant phenotype, and its enrichment in nuclei, all suggest TEG-1 also functions as a splicing factor. TEG-1, as well as its human homolog, CD2BP2, directly bind to UAF-1 U2AF65, a component of the U2 auxiliary factor. Conclusions TEG-1 functions as a splicing factor and acts to regulate the proliferation vs. meiosis decision. The interaction of TEG-1 CD2BP2 with UAF-1 U2AF65, combined with its previously described function in U4/U6.U5 tri-snRNP, suggests that TEG-1 CD2BP2 functions in two distinct locations in the splicing cascade.

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“The persistence of memory”—Hermaphroditism in nematodes

Ellis

2016

Molecular Reproduction Devel

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SUMMARYSelf-fertility has evolved many times in nematodes. This transition often produces an androdioecious species, with XX hermaphrodites and XO males. Although these hermaphrodites resemble females in most respects, early germ cells differentiate as sperm, and late ones as oocytes. The sperm then receive an activation signal, populate the spermathecae, and are stored for later use in self-fertilization. These traits are controlled by complex modifications to the sex-determination and sperm activation pathways, which have arisen independently during the evolution of each hermaphroditic species. This transformation in reproductive strategy then promotes other major changes in the development, evolution, and population structure of these animals.Mol. Reprod. Dev. 84: 144À157, 2017. ß 2016 Wiley Periodicals, Inc. The unique requirements that self-fertility places on the sexdetermination process in nematodes provide a window into the complex interactions between developmental and evolutionary forces.

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Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection

Cited by 28 publications

References 63 publications

The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

TEG‐1 CD2BP2 regulates stem cell proliferation and sex determination in the C. elegans germ line and physically interacts with the UAF‐1 U2AF65 splicing factor

“The persistence of memory”—Hermaphroditism in nematodes

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