Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects

Martínez-Valdivieso, Damián; Font, Rafael; Fernández-Bedmar, Zahira; Merinas-Amo, Tania; Gómez, Pedro Sánchez; Alonso-Moraga, Ángeles; Río-Celestino, Mercedes Del

doi:10.3390/nu9070755

Cited by 23 publications

(21 citation statements)

References 72 publications

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“…As a versatile and flexible model organism, zebrafish ( Danio rerio ) have been widely employed for biological and pathological studies, such as toxicity research [ 12 ] and pathological mechanism research [ 13 ]. Compared with the model organisms such as mice [ 14 ], rats [ 15 ], and Drosophila melanogaster [ 16 , 17 , 18 ], the zebrafish model organism has gathered a large number of advantages, including the optical clarity of the embryos and the ease for genetic manipulation, as well as the genetic similarity with humans [ 19 ]. Zebrafish was thought to be more appropriate for the research of angiocardiopathy than other models, due to the remarkable similarity of its cardiovascular system to humans [ 20 ] and the operation convenience.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Quality Marker (Q-Marker) of Danhong Injection by the Zebrafish Thrombosis Model

Zhao

Líu

et al. 2017

Molecules

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Quality-marker (Q-marker) is an emerging concept to ensure the quality and batch-to-batch consistency of Chinese medicine (CM). However, significant difficulties remain in the identification of Q-markers due to the unclear relationship between complex chemical compositions and the pharmacological efficacy of CM. In the present study, we proposed a novel strategy to identify the potential Q-marker of danhong injection (DHI) by an in vivo zebrafish thrombosis model. The anti-thrombotic effects of DHI and its major constituents were evaluated by the zebrafish model of arachidonic acid (AA)-induced thrombosis. The results indicated that DHI can attenuate tail venous thrombus and recover the decrease of heart red blood cell (RBC) intensity in a dose-dependent manner. The result that DHI prevented the formulation of thrombosis in zebrafish was also validated in the zebrafish thrombosis model with green fluorescence protein (GFP)-labeled hemoglobin. The major components of DHI, namely danshen (DS) and honghua (HH), as well as the major chemical constituents of DHI, also exerted anti-thrombotic effects, among which rosmarinic acid (RA) and p-coumaric acid (pCA) showed moderate anti-thrombotic effects. This is the first time that pCA from HH has been found as an active compound exerting an anti-thrombotic effect in a dose-dependent manner, whose IC50 value is approximately 147 μg/mL. By analyzing 10 batches of normal DHI samples and five abnormal samples by high-performance liquid chromatography (HPLC), we found the contents of pCA and RA can be positively correlated to the anti-thrombotic effect of DHI, suggesting that pCA and RA could be potential Q-markers of DHI to ensure batch-to-batch consistency. Our findings illustrated that discovering major active compounds from CM by in vivo pharmacological models can be a useful approach to identifying Q-markers of CM, and in vivo pharmacological models can be a potential tool to evaluate batch-to-batch consistency of CMs.

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Section: Introductionmentioning

confidence: 99%

Identification of a Quality Marker (Q-Marker) of Danhong Injection by the Zebrafish Thrombosis Model

Zhao

Líu

et al. 2017

Molecules

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“…In the light of the above considerations, the prepared emulsion is not only suitable for topical application, but even may be the best option to improve the acid mantle and skin barrier function. Indeed, Cucurbita pepo fruits are a common, high-value fruit vegetable [39], whose anti-genotoxicity and chemopreventive potential were recently explored [40]. The usage of pumpkin in cosmetic sector is not uncommon as its seeds, extracted by cold pressure, produce an oil rich in antioxidant and antimicrobial components.…”

Section: Resultsmentioning

confidence: 99%

Recovering Cucurbita pepo cv. ‘Lungo Fiorentino’ Wastes: UHPLC-HRMS/MS Metabolic Profile, the Basis for Establishing Their Nutra- and Cosmeceutical Valorisation

et al. 2019

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Food-waste is produced throughout all the food supply chain, with a large part already achieved at farm level. In fact, fruits and vegetables, which do not satisfy aesthetic demands, cannot be marketed, but their recovery could favour their valorisation for the obtainment of highly qualified goods. In this context, faulty zucchini fruits (cultivar ‘Lungo Fiorentino’), intended for disposal, were rescued as effective, inexpensive and bio-sustainable source for cosmeceutical purposes. Zucchini fruits underwent extraction and fractionation to obtain ZLF-O and ZLF-A extracts, which were chemically characterized by UHPLC-HRMS. ZLF-A extract, rich in flavonols and flavones, scavenged massively DPPH• and ABTS•+, and was not cytotoxic at doses up to 200 μ g/mL. Thus, ZLF-A was incorporated into a base cream formula. Zucchini-based emulsion was deeply screened for its antiradical properties and cytotoxicity towards human keratinocytes and fibroblasts. ZLF-A-enriched cream, whose chemical stability was assessed over time and mimicking different storage conditions, was further tested on reconstructed epidermis disks (EpiskinTM). The recovery of valuable chemical substances from zucchini agro-food waste, complying with the principles of valorisation and sustainable development, can represent a new market force for local farmers. Data acquired were eager to convey a suitable reuse of nutraceuticals rich zucchini waste.

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“… [ 13 , 17 – 19 ] Symphytum officinale L. Suelda consuelda FT1084 X L,R Anti-inflammatory, demulcent, hypotensive, stimulating tissue and nerves, astringent, diuretic and hemostatic. [ 17 , 20 ] Brassicaceae Mathiola incana (L.) W.T. Aiton Alhelí FT1250 X X F,L Anti-inflammatory, anti-flu, sedative, analgesic, cough and digestive.…”

Section: Methodsmentioning

confidence: 99%

“…Studies of cytotoxicity and genotoxicity/antigenotoxicity are rapid methods to assess the innocuousness and possible beneficial effects of single compounds or complex mixtures and foods [ 20 , 21 , 33 , 34 ]. The aim of this study is to evaluate the protective effects of this drink in southern Ecuador.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador

Bailón-Moscoso

Tinitana

Martínez-Espinosa

et al. 2017

BMC Complement Altern Med

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Background“Horchata” is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of this study was to evaluate the cytotoxicity and apoptotic/antigenotoxic effects of horchata as well as its mechanism.MethodsNine different varieties of horchata were prepared in the traditional way and then freeze-dried. Phytochemical screening tested for the presence of secondary metabolites using standard procedures and antioxidant activities. The cytotoxic activity was evaluated on cerebral astrocytoma (D-384), prostate cancer (PC-3), breast cancer (MCF-7), colon cancer (RKO), lung cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay. The pro-apoptotic effects were evaluated with Anexin V/Propidium Iodide and western blot of Bax, Bcl-2, TP53, and TP73. Induction and reduction of ROS were assessed by fluorimetry. Genotoxic and antigenotoxic effects were evaluated with a comet assay and micronuclei on binucleated cells.ResultsFive of nine horchatas had cytotoxic effects against D-384 while not affecting normal cells. These horchatas induce cell death by apoptosis modulated by p53/p73. In CHO-K1 cells, the horchatas decrease the damage induced by hydrogen peroxide and Mitomycin C measured in the comet and micronucleus assay respectively.ConclusionsThe IC50 range of effective horchatas in D-384 was 41 to 122 μg·mL−1. This effect may be related to its use in traditional medicine (brain tonic). On the other hand, immortalized Chinese hamster ovary cells (CHO-K1) and lymphocytes did not show a cytotoxic effect. The most potent horchata induced apoptosis via a p53/p73-mediated mechanism.The horchatas present antigenotoxic properties, which may be related to the antioxidant capacity. Future studies on horchata components are necessary to understand the interactions and beneficial properties.

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Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects

Cited by 23 publications

References 72 publications

Identification of a Quality Marker (Q-Marker) of Danhong Injection by the Zebrafish Thrombosis Model

Identification of a Quality Marker (Q-Marker) of Danhong Injection by the Zebrafish Thrombosis Model

Recovering Cucurbita pepo cv. ‘Lungo Fiorentino’ Wastes: UHPLC-HRMS/MS Metabolic Profile, the Basis for Establishing Their Nutra- and Cosmeceutical Valorisation

Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador

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