Roles for prostaglandins in the steroidogenic response of human granulosa cells to high-density lipoproteins

Chandras, Christina; Ragoobir, Jennifer; Barrett, Gemma E; Bruckdorfer, K. Richard; Graham, Annette; Abayasekara, D R E; Michael, Anthony E.

doi:10.1016/j.mce.2004.05.008

Cited by 11 publications

(9 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-density lipoprotein isolated from plasma was shown to deliver cholesterol to hGCs through a selective uptake pathway utilizing the scavenger receptor class B type 1 [39], as well as to stimulate progesterone synthesis in a process involving increased generation of luteotropic prostaglandins and cAMP [40,41]. The present study documents that (similar to plasma lipoprotein) FF-HDL is capable of stimulating ovarian steroidogenesis (Fig.…”

Section: Discussionsupporting

confidence: 68%

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Becker¹,

Otte²,

Robenek³

et al. 2011

Biology of Reproduction

View full text Add to dashboard Cite

Coordinated migration and progesterone production by granulosa cells is critical to the development of the corpus luteum, but the underlying mechanisms remain obscure. Sphingosine 1-phosphate (S1P), which is associated with follicular fluid high-density lipoprotein (FF-HDL), was previously shown to regulate ovarian angiogenesis. We herein examined the effects of S1P and FF-HDL on the function of granulosa lutein cells. Both FF-HDL and S1P induced migration of primary human granulosa lutein cells (hGCs) and the granulosa lutein cell line HGL5. In addition, FF-HDL but not S1P promoted progesterone synthesis, and neither of the two compounds stimulated proliferation of granulosa lutein cells. Polymerase chain reaction and Western blot experiments demonstrated the expression of S1P receptor type 1 (S1PR1), S1PR2, S1PR3, and S1PR5 but not S1PR4 in hGCs and HGL5 cells. The FF-HDL- and S1P-induced granulosa lutein cell migration was emulated by FTY720, an agonist of S1PR1, S1PR3, S1PR4, and S1PR5, and by VPC24191, an agonist of S1PR1 and S1PR3, but not by SEW2871 and phytosphingosine 1-phosphate, agonists of S1PR1 and S1PR4, respectively. In addition, blockade of S1PR3 with CAY1044, suramine, or pertussis toxin inhibited hGC and HGL5 cell migration toward FF-HDL or S1P, while blockade of S1PR1 and S1PR2 with W146 and JTE013, respectively, had no effect. Both FF-HDL and S1P triggered activation of small G-protein RAC1 and actin polymerization in granulosa cells, and RAC1 inhibition with Clostridium difficile toxin B or NSC23766 abolished FF-HDL- and S1P-induced migration. The FF-HDL-associated S1P promotes granulosa lutein cell migration via S1PR3 and RAC1 activation. This may represent a novel mechanism contributing to the development of the corpus luteum.

show abstract

Section: Discussionsupporting

confidence: 68%

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Becker¹,

Otte²,

Robenek³

et al. 2011

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…The bovine CL synthesises APOA1, the main component of HDL, across the oestrous cycle and pregnancy (NdikumMoffor et al 1997). Thus, APOA1 stimulates P 4 production in the CL and promotes PGE 2 production (a luteotrophic PG) (Chandras et al 2004). In our study, APOA1 decreased from early to late luteal phase during CL regression but was highly expressed in the early CL of pregnancy at the time of embryo implantation (day 16) and post-implantation (day 20).…”

Section: Steroidogenesissupporting

confidence: 50%

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Arianmanesh¹,

McIntosh²,

Lea³

et al. 2011

Journal of Endocrinology

View full text Add to dashboard Cite

Progesterone (P 4 ) secreted by the corpus luteum (CL) is critical for in utero embryo survival and development, although CL proteins are key regulatory factors during the luteal phase. We, therefore, characterised protein expression patterns in ovine CL of pregnancy (days 12, 16 and 20) compared with those of controls, CL of oestrous cycle (days 12 and 16), using two-dimensional gel electrophoresis (2DE) gel-based proteomics. Proteins in 24 significantly altered spots were identified by tandem mass spectroscopy. At the time of embryo implantation (day 16), 77 spots were up-regulated and 101 spots were down-regulated in CL of pregnancy compared with regressed CL. Vimentin, lamin A/C (LMNA), [Mn] superoxide dismutase (SOD2), isocitrate dehydrogenase 1, annexin A1 and elongation factor Tu, mitochondrial (TUFM) altered during CL regression, whereas glutathione S-transferase A1, apolipoprotein A-1, myxovirus resistance protein 1, ornithine aminotransferase and enoyl-CoA hydratase, mitochondrial (ECHS1) tended to be altered during CL maintenance. biliverdin reductase B (BLVRB), FDXR, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (GNB2) and cytochrome bc1 complex subunit 1, mitochondrial (UQCRC1) showed divergent expression during CL regression and maintenance. The expression of two representative proteins, SOD2 and BLVRB, by western blot increased in CL of non-pregnant ewes on day 16 compared with that on day 12. SOD2 and BLVRB were localised in the large and small luteal cells and endothelial cells of CL over peri-implantation periods. 2DE gel and mass spectrometry have been used, for the first time, to study ovine CL function. We have identified proteins involved in key pathways, including oxidative stress, steroidogenesis, signal transduction and apoptosis, which have not previously been associated with changes occurring in the CL during the peri-implantation period. These proteins are most likely involved with mechanisms allowing the CL to produce P 4 during early pregnancy.

show abstract

“…Although the mechanisms underlying this effect are not clear they are consistent with the observations of Bao et al (1995), who also found that HDL, but not LDL, increased P 4 production in bovine theca cells between 96 and 144 h culture. Furthermore, this effect may be induced by the underlying protein molecule as Chandras et al (2004) found that lipid-free apolipoprotein-A1 increased P 4 production in human granulosa-lutein cells following 3 days of culture. The study of Wonnacott et al (2010) also revealed that there is no net uptake of FA from HDL by sheep embryos, so that FA composition was similar to embryos cultured in the presence of FAF-BSA.…”

Section: Discussionmentioning

confidence: 99%

Effects of omega-3 and -6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism

Hughes

Kwong²,

Li³

et al. 2011

Reproduction

View full text Add to dashboard Cite

We previously reported increased follicular fluid progesterone (P 4 ) concentrations in ewes fed an n-3 compared to an n-6 polyunsaturated fatty acid (PUFA)-enriched diet, but detected no differential effect of n-3 and n-6 PUFA-enriched high-density lipoproteins (HDL) on granulosa cell (GC) steroidogenesis in vitro. Moreover, net n-6 PUFA-enriched HDL reduced early embryo development, but in the absence of a net uptake of FA. Consequently, we hypothesised that a) effects of n-3 PUFA on ovarian steroidogenesis are mediated by theca rather than GCs and b) during embryo culture lipids are acquired solely from the albumin fraction of serum, so that albumin-delivered n-3 and n-6 PUFA exert a greater differential effect on embryo development than either low-density lipoprotein (LDL)-or HDL-delivered PUFA. Data confirmed that n-3 PUFA increases P 4 production solely in theca cells and that this is associated with an increase in STAR transcript expression. Furthermore, LDL-and HDL-delivered n-3 PUFA are equally efficacious in this regard during the first 96 h of culture, but thereafter only HDL-delivered n-3 PUFA induces this effect in partially luteinised theca cells. We also demonstrate that albumin is the sole serum fraction that leads to a net uptake of FA during embryo culture. PUFA-enriched serum and albumin increased the yield of morphologically poorer quality blastocysts with increased transcript expression for the antioxidant enzyme SOD1. Important differential effects of n-3 and n-6 PUFA on ovarian steroidogenesis acting solely on theca cells are identified, but differential effects of PUFA on embryo development are less apparent.

show abstract

Roles for prostaglandins in the steroidogenic response of human granulosa cells to high-density lipoproteins

Cited by 11 publications

References 68 publications

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Effects of omega-3 and -6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism

Contact Info

Product

Resources

About