Roles of Rack1 Proteins in Fungal Pathogenesis

Zhang, Xue; Jain, Rashmi; Li, Guotian

doi:10.1155/2016/4130376

Cited by 8 publications

(9 citation statements)

References 79 publications

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“…To a large extent, our experiments enable the analysis of the phosphorylation reactions at amino acid resolution (reliably for all phospho-peptides with AScores > 13, meaning a p -value for the phospho-site localization <0.05). Regarding the G-proteins, RACK1-homologs have been demonstrated to function as an alternative Gβ in several fungi and are well-known for their additional role as scaffolding proteins for numerous processes in eukaryotes (Ron et al, 2013; Zhang et al, 2016). In N. crassa , the RACK1 homolog cross-pathway control-2 (CPC-2) is required for proper expression of numerous amino acid biosynthetic enzymes in response to starvation for a single amino acid (Muller et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa

et al. 2019

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Fungal plant cell wall degradation processes are governed by complex regulatory mechanisms, allowing the organisms to adapt their metabolic program with high specificity to the available substrates. While the uptake of representative plant cell wall mono- and disaccharides is known to induce specific transcriptional and translational responses, the processes related to early signal reception and transduction remain largely unknown. A fast and reversible way of signal transmission are post-translational protein modifications, such as phosphorylations, which could initiate rapid adaptations of the fungal metabolism to a new condition. To elucidate how changes in the initial substrate recognition phase of Neurospora crassa affect the global phosphorylation pattern, phospho-proteomics was performed after a short (2 min) induction period with several plant cell wall-related mono- and disaccharides. The MS/MS-based peptide analysis revealed large-scale substrate-specific protein phosphorylation and de-phosphorylations. Using the proteins identified by MS/MS, a protein-protein-interaction (PPI) network was constructed. The variance in phosphorylation of a large number of kinases, phosphatases and transcription factors indicate the participation of many known signaling pathways, including circadian responses, two-component regulatory systems, MAP kinases as well as the cAMP-dependent and heterotrimeric G-protein pathways. Adenylate cyclase, a key component of the cAMP pathway, was identified as a potential hub for carbon source-specific differential protein interactions. In addition, four phosphorylated F-Box proteins were identified, two of which, Fbx-19 and Fbx-22, were found to be involved in carbon catabolite repression responses. Overall, these results provide unprecedented and detailed insights into a so far less well known stage of the fungal response to environmental cues and allow to better elucidate the molecular mechanisms of sensory perception and signal transduction during plant cell wall degradation.

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Section: Discussionmentioning

confidence: 99%

Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa

et al. 2019

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“…However, other studies have reported the potency of 8-hydroxyquinoline as a scaffold for drug design to get novel microbial bioactive derivatives (Pippi et al, 2019). It is hypothetical that the key proteins and signal transduction pathways in plant pathogenic fungi could be the targets susceptible to preservatives such as 8-HQS in microbial inhibition (Zhang et al, 2016). The adverse effects of cold storage without pulsing of cut flowers have been reported (Chore et al, 2019).…”

Section: Days Of Storagementioning

confidence: 99%

Influence of pulsing and wet cold storage on the vase water microbial profiles and overall quality of cut gladioli

et al. 2021

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Occlusion of the stem vasculature by microorganisms that proliferate in the vase water, or the plant vessels, leads to water stress symptoms that reduce postharvest quality of cut flowers. This study aimed to determine the effects of pulsing and wet-cold storage on the microbial profiles in cut Gladiolus grandiflorus L. cv. Fado. Pulsing treatments of 600-ppm 8-hydroxyquinoline sulfate plus 5% sucrose solution versus distilled water were administered before wet cold storage periods of 0-5 days in cut Gladiolus, previously grown from corms under open field. A two-by-six factorial experiment embedded in a completely randomized design with four replicates was accomplished. Proc GLM in two-way Anova was adopted, and the means were separated using Tukey’s test at a 5% level of significance. The pulsing treatment of 600 ppm 8-HQS plus 5% sucrose, the wet cold storage duration and their interactive effects significantly (P˂0.0209; ˂0.0001 and ˂0.0001 respectively) affected the means of the colony-forming units in the vase water of cut Gladiolus at senescence. The prolonged vase life of cut gladioli spikes was associated with decreased microbial proliferation as influenced by pulsing and wet storage duration of up to 4 days. Data generated from this study will improve existing technologies related to the quality and market value of this Gladiolus cultivar.

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“…As a scaffold protein, RACK1 is known to regulate many cellular processes such as cell proliferation, signaling, development, migration, and apoptosis (for review [1,2]). In addition, RACK1 has increasingly been implicated in many viral, bacterial, fungal infections as well as in human diseases, including cancer, Alzheimer, Parkinson's, and alcohol addiction [9,[11][12][13][14][15]. Recent studies demonstrated that RACK1 regulates many pathogenic protozoan/microbial/fungal infections resulting in malaria [16], scarlet-like fever [11], and histoplasmosis [15].…”

Section: Introductionmentioning

confidence: 99%

Host-Targeted Antivirals Inhibit RACK1-mediated IRES Activities in HIV-1 Infection

Malli¹,

Kumari²,

Dubrovsky³

et al. 2021

Preprint

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Host ribosome-associated scaffold protein Receptor for Activated C Kinase 1 (RACK1) is utilized by a diverse group of human viruses for Internal Ribosomal Entry Sites (IRES) – mediated translation of viral mRNAs. We recently reported inhibition of herpes virus by small molecules targeting the RACK1 functional site. Here, we tested these molecules against HIV-1 and HCV, as HIV-1 contains two potential IRES sites and HCV translation occurs exclusively through IRES. Compounds significantly downregulated activities of HIV-1- and HCV-related dicistronic reporter constructs in transfected HEK293T cells. The compounds also strongly downregulated production of the HIV-1 capsid protein p24 in HIV-infected cells, as well as production of HIV-1 Gag precursor p55 and p55-derived proteins p24 and p17 in cells infected with the HIV-1 virus. Hepatitis C virus (HCV) IRES activities were also significantly inhibited by RACK1 inhibitor compounds. Since a number of human and plant pathogenic viruses are reported to use IRES, the RACK1 compounds can be established as broad host-targeted antivirals.

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Roles of Rack1 Proteins in Fungal Pathogenesis

Cited by 8 publications

References 79 publications

Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa

Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa

Influence of pulsing and wet cold storage on the vase water microbial profiles and overall quality of cut gladioli

Host-Targeted Antivirals Inhibit RACK1-mediated IRES Activities in HIV-1 Infection

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