Rolling Circle Replication and Transcription Processes in Geminiviruses

Sharma, Nivedita; Ruhel, Rajrani

doi:10.1007/978-3-030-18248-9_2

Cited by 3 publications

(2 citation statements)

References 117 publications

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“…In some situations excised unit length cloned DNA bearing single copies of virus genome act as an infectious clone of certain plant viruses [19][20][21]. Previous studies had described that most infectious clones entail tandem repeat constructs of geminivirus/begomovirus genomes to extend their pathogenicity [22][23][24][25], which makes cloning procedure more complex. The process make multimeric constructs of begomoviruses for tickly selection and design of cloning sites, pursued by aggregation of viral genomic fragments yielded by restriction endonucleases has become an exhausting phenomenon [26,27].…”

Section: Introductionmentioning

confidence: 99%

Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis

et al. 2021

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Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.

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Section: Introductionmentioning

confidence: 99%

Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis

et al. 2021

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“…Infections caused by fungi, bacteria, and phytoplasma are only a few of the many viral diseases that affect it. The most significant and damaging viral pathogen in many regions of the world is the tomato leaf curl virus (ToLCV), a geminivirus, which is responsible for all documented viral diseases in tomatoes [20][21][22][23][24][25][26][27]. Based on the genome organization, host range, phylogenetic relationships, and insect vectors, geminiviruses have been classified into nine genera: Becurtovirus (two species), Begomovirus (>320 species), Curtovirus (three species), Mastrevirus (>30 species), Eragrovirus (one species), Topocuvirus (one species), and Turncurtovirus (one species).…”

Section: Viruses Crops Affected and Damage Causedmentioning

confidence: 99%

Leaf Curl Disease a Significant Constraint in the Production of Tomato: Impact, Challenges, and Management

Chintapalli¹,

Rayalcheruvu²

2022

Tomato - From Cultivation to Processing Technology

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Insect-borne plant viruses cause huge yield loss in the world’s most important crops. Understanding viral transmission mechanisms involves defining plant virus receptors inside their insect vectors. Tomato leaf curl virus (ToLCV) is the most devastating virus for worldwide tomato production. Understanding the biology of ToLCV and devising management techniques are critical in combating this global threat. Researchers are looking into using advanced technologies to detect plant viruses quickly and handle them properly for long-term agriculture. This review’s main goal is to highlight management solutions for effectively combating ToLCV outbreaks and worldwide spread. Resistance genes for plant viruses in agriculture have been identified using morphological, biochemical, and molecular markers from the ancient to the present era. Such techniques are extremely basic. Traditional virus identification methodologies should be integrated with current and advanced tools for efficient virus improvement in crops. This review’s main goal is to highlight management solutions for effectively combating ToLCV outbreaks and worldwide spread. For this aim, we focus on the impact of ToLCV on the world’s agriculture and the significance of recent advances in our comprehension of its interactions with its host and vector. Another important topic is the role of mutations and recombination in shaping the ToLCV genome’s evolution and regional distribution.

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The rising threat of geminiviruses: molecular insights into the disease mechanism and mitigation strategies

et al. 2023

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Rolling Circle Replication and Transcription Processes in Geminiviruses

Cited by 3 publications

References 117 publications

Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis

Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis

Leaf Curl Disease a Significant Constraint in the Production of Tomato: Impact, Challenges, and Management

The rising threat of geminiviruses: molecular insights into the disease mechanism and mitigation strategies

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