Root and Shoot Initiation in Aspen Callus Cultures

Wolter, Karl E.

doi:10.1038/219509a0

Cited by 77 publications

(23 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The compound represses cell differentiation and morphogenesis while, for example, NAA promotes the process (6,18). The increase in ethylene production caused by auxins may relate to the cell differentiation process.…”

Section: Resultsmentioning

confidence: 99%

Ethylene Production by Plant Cell Cultures

Gamborg

LaRue

1971

Plant Physiol.

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Ethylene Production by Plant Cell Cultures

Gamborg

LaRue

1971

Plant Physiol.

View full text Add to dashboard Cite

“…Poplars have a small genome size (C value = 0.7 pg; ref. 3), are easily propagated vegetatively, and display developmental plasticity in cell culture (4)(5)(6). These traits, coupled with susceptibility to Agrobacterium-mediated transformation (7,8) and techniques to regenerate transgenic trees (9,10), make poplar an attractive model system for forest tree molecular biology.…”

mentioning

confidence: 99%

Systemic accumulation of specific mRNAs in response to wounding in poplar trees.

Parsons

Bradshaw

Gordon

1989

Proc. Natl. Acad. Sci. U.S.A.

114

View full text Add to dashboard Cite

cDNAs for several transcripts that accumulate systemically in response to mechanical wounding in a hybrid poplar have been cloned. The corresponding mRNAs become abundant in the unwounded upper leaves of poplar trees whose lower leaves have been damaged. Two of the cDNA clones have been characterized by Northern and Southern blotting, and their nucleotide sequences have been determined. These clones, designated win6 (wound-inducible) and win8, are members of multigene families encoding proteins with a high degree of similarity to chitinases from bean, tobacco, and barley. This initial demonstration of a systemic response to wounding in trees provides an approach to study defense mechanisms in woody plants at the biochemical, physiological, and ecological levels.

show abstract

“…Shoot meristems can also synthesize cytokinins, but the production of these compounds is distributed more locally (Böhner and Gatz 2001, ShimizuSato and Mori 2001, Sakakibara 2006. Cytokinins mainly inhibit root initiation and promote shoot initiation (Winton 1971, Wolter 1968, Van Staden and Harty 1988, Werner et al 2001, Schmülling 2002. The influence of cytokinins may thus depend on their concentration and on the stage of the root initiation process (Okoro and Grace 1978, Jarvis 1986, Van Staden and Harty 1988, Cline 1999, Schmülling 2002.…”

Section: Endogenous Control Of Adventitious Organogenesismentioning

confidence: 99%

Multiplication of hybrid aspen (<em>Populus tremula</em> L. x <em>P. tremuloides</em> Michx.) from cuttings

Stenvall¹

2006

Diss. For.

View full text Add to dashboard Cite

The primary aim of the present study was to find an efficient and simple method of vegetative propagation for producing large numbers of hybrid aspen (Populus tremuloides L. x P. tremula Michx.) plants for forest plantations. The key objectives were to investigate the main physiological factors that affect the ability of cuttings to regenerate and to determine whether these factors could be manipulated by different growth conditions. In addition, clonal variation in traits related to propagation success was examined. According to our results, with the stem cutting method, depending on the clone, it is possible to obtain only 1−8 plants from one stock plant per year. With the root cutting method the corresponding values for two-year-old stock plants are 81−207 plants. The difference in number of cuttings between one-and two-yearold stock plants is so pronounced that it is economically feasible to grow stock plants for two years. There is no reason to use much older stock plants as a source of cuttings, as it has been observed that rooting ability diminishes as root diameter increases. Clonal variation is the most important individual factor in propagation of hybrid aspen. The fact that the efficiently sprouted clones also rooted best facilitates the selection of clones for large-scale propagation. In practice, root cuttings taken from all parts of the root system of hybrid aspen were capable of producing new shoots and roots. However, for efficient rooting it is important to use roots smaller than one centimeter in diameter. Both rooting and sprouting, as well as sprouting rate, were increased by high soil temperature; in our studies the highest temperature tested (30ºC) was the best. Light accelerated the sprouting of root cuttings, but they rooted best in dark conditions. Rooting is essential because without roots the sprouted cutting cannot survive long. For aspen the criteria for clone selection are primarily fiber qualities and growth rate, but ability to regenerate efficiently is also essential. For large-scale propagation it is very important to find clones from which many cuttings per stock plant can be obtained. In light of production costs, however, it is even more important that the regeneration ability of the produced cuttings be high.Keywords: vegetative propagation, regeneration ability, stem cuttting, root cutting. ACKNOWLEDGEMENTSI am very grateful to my supervisor, docent Pertti Pulkkinen, who offered me the possibility to work in this project. He has always found time to comment of the manuscripts, discuss problems related to my experiments, or whatever that I had on my mind. He has also introduced me to the ins and outs of science, advised me to take a part in many international conferences and encouraged me in applying grants.During the years, I have found many good friends in Haapastensyrjä, Finnish Forest Research Institute´s Breeding Station in Läyliäinen. Special thanks belong to Pilvi Jokiniemi and Päivi Selin, who worked with me for several summers. We had hilarious days in green...

show abstract

Root and Shoot Initiation in Aspen Callus Cultures

Cited by 77 publications

References 2 publications

Ethylene Production by Plant Cell Cultures

Ethylene Production by Plant Cell Cultures

Systemic accumulation of specific mRNAs in response to wounding in poplar trees.

Multiplication of hybrid aspen (<em>Populus tremula</em> L. x <em>P. tremuloides</em> Michx.) from cuttings

Contact Info

Product

Resources

About