Root canal microorganisms and their antibiotic susceptibility in patients with persistent endodontic infections, with and without clinical symptoms

Medina-Palacios, Sara Edith; Vitales-Noyola, Marlen; López-González, Emmanuel; Amaro, Ana María González; Méndez-González, Verónica; Pozos‐Guillén, Amaury

doi:10.1007/s10266-020-00580-2

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…Vigil et al isolated Clostridium from 22 refractory periapical cases via growth culture [54]. Medina-Palacios et al identified that Clostridium was one of the most common genera in refractory apical periodontitis [55]. One possible mechanism that Clostridium contributed to destruction is degrading Type IV collagen, which is the major component of basement membranes, via proteinases [56].…”

Section: Discussionmentioning

confidence: 99%

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

Sun

Guo

et al. 2021

Journal of Immunology Research

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Objective. The primary aim of this investigation was to analyze the microbiome in patients with combined periodontal-endodontic lesions. Method. Patients with loose and/or painful teeth referred for treatment from March 2020 to December 2020 in the First People’s Hospital of Jinzhong were recruited. Samples were collected from teeth diagnosed as chronic periodontics (PE), ulcerative pulpitis (PU), and retrograde pulpitis (RE). Genomic DNA was extracted. The quantitative polymerase chain reaction, targeting the 16S ribosomal RNA (rRNA), was adopted for the quantification of bacteria. Then, the V3-V4 hypervariable regions of the 16S rRNA gene were amplified and subjected to next-generation sequencing. The statistical analysis was performed by R software (V3.5.1). Results. A total of 57 qualified samples were collected from 48 patients and analyzed (7 PE, 21 PU, and 19 RE). By linear discriminant analysis effect size, Kingella and Barnesiella were significantly increased in the periodontal pocket of retrograde pulpitis (RE-PE), compared with PE. The relative abundance of Clostridiales Incertae Sedis XI, Fusobacteriaceae, Fusobacterium, Parvimonas, Micrococcaceae, and Rothia was significantly increased in the pulp of retrograde pulpitis (RE-PU) than PU and RE-PE. Prevotella, Leptotrichia, Porphyromonas, Streptococcus, and Fusobacterium are consistently at a high abundance, across PU, RE-PE, and RE-PU. Conclusion. The current study highlighted the evidence that a specific microbial community is associated with the occurrence of retrograde pulpitis. The microenvironment of the root canal and pulp chamber will select microbiota. This study offered insights into the pathogenesis of retrograde pulpitis.

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Section: Discussionmentioning

confidence: 99%

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

Sun

Guo

et al. 2021

Journal of Immunology Research

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“…Although antimicrobial compounds have been reported to decrease the occurrence of dental disease, the use of antibiotics and chemical bactericides can have a negative impact on the bacterial flora of the oral cavity and intestinal tract [15,16]. Since pathogens can acquire resistance against different antibiotics, agents that are characterized for having remarkable antibacterial activity and do not develop resistance are now in high demand [17,18]. Bacterial growth in the oral cavity is the main cause of secondary caries [19].…”

Section: Introductionmentioning

confidence: 99%

A Study of Zn-Ca Nanocomposites and Their Antibacterial Properties

Torres-Ramos

Martín-Camacho

González

et al. 2022

IJMS

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This study aimed to develop Ca2+ doped ZnO nanoparticles (NPs) and investigate their antibacterial properties against microorganisms of dental interest. Zn-Ca NPs were synthesized by the sol-gel method with different concentrations of Ca2+ (1, 3, and 5 wt. %) and subsequently characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-vis spectroscopy and Fourier transform infrared spectroscopy (FT-IR). The Kirby–Bauer method was used to measure antibacterial effects. NPs showed the wurzite phase of ZnO and bandgap energies (Eg) from 2.99 to 3.04 eV. SEM analysis showed an average particle size of 80 to 160 nm. The treatments that presented the best antibacterial activity were Zn-Ca 3% and Zn-Ca 5%. ZnO NPs represent an alternative to generate and improve materials with antibacterial capacity for dental applications.

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“…Chronic, asymptomatic inflammatory lesions around the apex of a tooth with necrotic dental pulp or an insufficient passageway treatment can develop unnoticed by the patient, and remain so for years (2). The course of disease is modulated by both the virulence of the microbiota established in the root canal space and the capacity of the immune system to curb the infection (3,4). Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage (5).…”

Section: Introductionmentioning

confidence: 99%

Evaluation Of Knowledge, Attitude and Practice of Final Year and Internship Students Towards the Use of Devitalising Agent in Endodontics

2022

JETT

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Introduction: Infections of the root canal space and their sequelae may be extremely painful and potentially dangerous, yet they may not always necessarily be. Many general dental practitioners find management of the inflamed pulp challenging in their routine dental practice. First step within the mortal endodontic methods of treatment is positioning of devitalizing medicine. The devitalizing agents can compose formaldehyde, cresol, paraformaldehyde or some arsenic compounds. Thus, this study aims to analyze knowledge, attitude and practice of final year and internship students regarding the use of devitalizing agents in their respective practice. Materials And Method: A total of 100 practicing final year and internship students were randomly chosen. The questionnaire was about the general information of the different aspects of devitalizing agents. The collected data was subjected to SPSS. Descriptive statistics was drawn with respective percentages to have a comparative overview. Result: The response rate was 99% of which effective and complete replies were 100%. 73% of the students observed post operative pain. 55% respondents used paraformaldehyde containing paste. 38% of the students were not aware of the complications of devitalizing agentsP value is 0.352; DF: 3; p value: 0.615 ie (p > 0.05). Hence the association was not statistically significant. Conclusion: We concluded that final year and internship students do use pulp devitalizing agents despite possessing knowledge related to the complications.

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Root canal microorganisms and their antibiotic susceptibility in patients with persistent endodontic infections, with and without clinical symptoms

Cited by 10 publications

References 35 publications

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

A Study of Zn-Ca Nanocomposites and Their Antibacterial Properties

Evaluation Of Knowledge, Attitude and Practice of Final Year and Internship Students Towards the Use of Devitalising Agent in Endodontics

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