Root-end Filling Materials Alter Fibroblast Differentiation

Bonson, S.; Jeansonne, Billie G.; Lallier, Thomas E.

doi:10.1177/154405910408300511

Cited by 143 publications

(122 citation statements)

References 24 publications

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“…Although the effect of MTA on MC3T3-E1 cells was minimal, a significant difference between the groups was found on day 3. Our results were consistent with previously reports that MTA enables proliferation of various cell types [4][5][6][7][8] , and the excellent biocompatibility of MTA was confirmed.…”

Section: Discussionsupporting

confidence: 83%

“…Thus, MTA has been widely used in endodontic treatments, including direct pulp capping, root-end filling, perforation repair, and apexification [1][2][3] . MTA bioactivity involves the ability to enhance cell proliferation, migration, and mineralization in various cell types [4][5][6][7][8][9] . MTA contains primarily calcium silicate, which is converted into calcium hydroxide upon contact with tissue fluid.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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“…Furthermore, MTA might stimulate PDL fibroblasts to display the osteogenic phenotype and promote the production of osteonectine, osteopontine, and increase alkaline phosphatase levels [38,39]. Our data shows that all treatment case was improved the percussion pain after until 6 months.…”

Section: Discussionsupporting

confidence: 55%

The clinical evaluation of vertical root fracture after endodontic treatment with mineral trioxide aggregate

Tani‐Ishii¹,

Mutoh²,

Muromachi³

et al. 2017

Integr Mol Med

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“…This may be due to the excellent biocompatibility of material and the ability of osteoblastic cells to connect and spread on its surface. 24 In addition to stimulating adhesion and cell proliferation 18 , the expression of alkaline phosphatase by fibroblasts, 4 osteocalcin and accessed by following the link in the citation at the bottom of the page. …”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

Silva

Kim

Luczyszyn

et al. 2015

International Journal of Oral and Maxillofacial Surgery

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Eighty Wistar rats were divided into 4 groups and examined over a period of 2 or 8 weeks. First, a surgical defect was created in the calvaria using a 6 mm diameter trephine drill. The cavity was then treated with BCP (biphasic calcium phosphate), MTA, or the combination of the two. In the control, the cavity was allowed to clot. After 2 or 8 weeks, animals were sacrificed and the bones were subjected to a histological evaluation and immunohistochemical staining to evaluate areas of new osteoid tissue and new bone tissue as well as the percentage of labeled cells that utilize anti-BMPR1B antibodies. Twoway ANOVA showed statistical significant diference in all dependent variable (area of new osteoid tissue; area of new bone and % of immunostainning) within group p=0.00000, time p=0.00000 and with the interaction of both p=0.0000. MTA group at 8 weeks showed the highest amount of osteoid tissue (6.25 ± 0.30 mm 2 ). The same group also exhibited the highest amount of bone tissue formation (3.98 ± 0.14 mm 2 ). In contrast, both 2-week MTA samples (40.73 ± 2.50%) and 2-week BCP+MTA samples (40.30 ± 1.81%) exhibited the highest percentage of stained cells. Best results in terms of the area of osteoid and bone tissue formation and the percentage of BMPR1B markings were observed for the MTA group, confirming that the combination of BCP and MTA does not result in a significant improvement.

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Root-end Filling Materials Alter Fibroblast Differentiation

Abstract: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).

Cited by 143 publications

References 24 publications

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

The clinical evaluation of vertical root fracture after endodontic treatment with mineral trioxide aggregate

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

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