Root hairiness: effect on fluid flow and oxygen transfer in hairy root cultures

Shiao, Tien-Li; Doran, Pauline M.

doi:10.1016/s0168-1656(00)00316-3

Cited by 36 publications

(16 citation statements)

References 24 publications

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“…Hairy roots were initiated and grown in liquid Gamborg's B5 medium with 3% sucrose as described previously (Shiao and Doran, 2000). The A. thaliana hairy roots were extremely ®ne and formed dense clumps; in liquid medium, the root clumps grew with the appearance and texture of wet cotton wool.…”

Section: Hairy Root Induction and Culturementioning

confidence: 99%

“…Oxygen uptake rates were measured in the absence of external hydrodynamic boundary layer eects in a singletube recirculation bioreactor as described previously (Williams and Doran, 1999;Shiao and Doran, 2000). Operation at a super®cial liquid velocity of 1.0 cm s )1 eliminated hydrodynamic boundary layers around the roots according to the criteria outlined previously (Williams and Doran, 1999), and reduced the conversion per pass of the bioreaction tube to 0.3±1.6%.…”

Section: Oxygen Uptake Measurementsmentioning

confidence: 99%

“…Various aspects of root morphology such as diameter, branchedness, and rigidity (Carvalho et al, 1997) and the proli®cacy and length of the root hairs (Bordonaro and Curtis, 2000;Shiao and Doran, 2000) can aect¯uid¯ow resistance, and therefore external oxygen transfer, in hairy root cultures. Because dissolved oxygen tensions may be signi®cantly lower inside hairy root clumps than in the bulk medium (Yu et al, 1997), and as hairy roots have relatively high external critical oxygen tensions for growth and respiration (Shiao and Doran, 2000;Williams and Doran, 1999), delivery of oxygen is likely to become inadequate in submerged root systems. The consequences of oxygen limitation in hairy root cultures include reduced nutrient uptake and lower rates of growth and product synthesis (Kanokwaree and Doran, 1998;Kwok and Doran, 1995;McKelvey et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations

Shiao

Ellis

Dolferus

et al. 2002

Biotech & Bioengineering

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Overexpression of Arabidopsis thaliana genes for the fermentation enzymes, alcohol dehydrogenase and pyruvate decarboxylase, improved the tolerance of A. thaliana hairy roots to low oxygen conditions. Whereas the specific growth rate of untransformed hairy roots in shake flasks and in a multiple-tube recirculation bioreactor declined significantly with decreasing oxygen tension down to 25% air saturation, growth of the transformant root lines was maintained at rates similar to those achieved with full aeration. This work demonstrates that altering the expression of selected genes involved in anaerobic metabolism can alleviate the problems of oxygen deficiency in hairy root cultures caused by poor mixing and mass transfer conditions.

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Section: Hairy Root Induction and Culturementioning

confidence: 99%

Section: Oxygen Uptake Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations

Shiao

Ellis

Dolferus

et al. 2002

Biotech & Bioengineering

Self Cite

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“…Tylophora indica transformed with A. rhizogenes A4 showed a more rapid growth and higher branching on liquid medium than when cultured on solid medium . Hairy roots growing in liquid medium in shake flasks may enhance root growth due to increased oxygen transfer generated by the constant shaking, even though oxygen is limited in liquid medium (Shaoxiong et al, 1997;Shiao and Doran, 2000).…”

Section: Growing Medium Optimizationmentioning

confidence: 99%

“…Hairy roots rely on oxygen for energy generation and other metabolic functions; and oxygen supply may also directly influence the growth and production of secondary metabolites (Shaoxiong et al, 1997;Shiao and Doran, 2000).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Orellana¹,

Ferri²

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Bioreactor technology: A novel industrial tool for high‐tech production of bioactive molecules and biopharmaceuticals from plant roots

Sivakumar

2006

Biotechnology Journal

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Plants are the richest source for different bioactive molecules. Because of the vast number of side effects associated with synthetic pharmaceuticals, medical biotechnologists turned to nature to provide new promising therapeutic molecules from plant biofactories. The large-scale availability of the disease- and pesticide-free raw material is, however, restricted in vivo. Many bioactive plant secondary metabolites are accumulated in roots. Engineered plants can also produce human therapeutic proteins. Vaccines and diagnostic monoclonal antibodies can be won from their roots, so that engineered plants hold immense potential for the biopharmaceutical industry. To obtain sufficient amounts of the plant bioactive molecules for application in human therapy, adventitious and hairy roots have to be cultured in in vitro systems. High-tech pilot-scale bioreactor technology for the establishment of a long-term adventitious root culture from biopharmaceutical plants has recently been established. In this review, I briefly discuss a technology for cultivating bioactive molecule-rich adventitious and hairy roots from plants using a high-tech bioreactor system, as well as the principles and application of genome-restructuring mechanisms for plant-based biopharmaceutical production from roots. High-tech bioreactor-derived bioactive phytomolecules and biopharmaceuticals hold the prospect of providing permanent remedies for improving human well-being.

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Root hairiness: effect on fluid flow and oxygen transfer in hairy root cultures

Cited by 36 publications

References 24 publications

Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations

Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Bioreactor technology: A novel industrial tool for high‐tech production of bioactive molecules and biopharmaceuticals from plant roots

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