Root Nodule and Rhizosphere Bacteria for Forage Legume Growth Promotion and Disease Management

Altier, Nora; Beyhaut, Elena; Pérez, Carlos A.

doi:10.1007/978-3-642-37241-4_7

Cited by 8 publications

(7 citation statements)

References 65 publications

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“…Most legumes establish symbiosis with rhizobia, bacteria capable of fixing atmospheric nitrogen in a process referred to as biological nitrogen fixation (BNF). BNF allows saving nitrogenous fertilizers, with economic, and environmental benefits (Altier et al, 2013). The demand for sustainable agricultural practices has thus reinforced the attention in BNF (Sulieman and Tran, 2015), particularly in symbioses involving economically important food, and forage crops (Lindström et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Selection of Competitive and Efficient Rhizobia Strains for White Clover

et al. 2019

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The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover ( Trifolium repens ) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N 2 in clover and tagged with gus A was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N 2 ( 15 N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the native-naturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains.

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Section: Introductionmentioning

confidence: 99%

Selection of Competitive and Efficient Rhizobia Strains for White Clover

et al. 2019

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“…For instance, in India, a common practice is to use an unknown formulation in the field. In our country, every inoculant has to be thoroughly identified and tested before its use in the field (43) . For this reason, our aim focused on the characterization and testing of some physiological and biochemical characteristics to evaluate their growth in vitro.…”

Section: Potential Inoculant Evaluationmentioning

confidence: 99%

Potential cyanobacterial inoculants for rice described from a polyphasic approach

2020

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Ten heterocyst cyanobacteria isolated from a temperate ricefield in Uruguay were characterized using a polyphasic approach. Based on major phenotypic features, the isolates were divided into two different morphotypes within the Order Nostocales, filamentous without true branching. The isolates were also phylogenetically evaluated by their 16S rRNA and hetR gene sequences. Although the morphological classification of cyanobacteria has not always been supported by the analysis of the 16S rRNA gene, in this case the morphological identification agreed with the 16S rRNA gene phylogenetic analysis and the ten isolates were ascribed at the genus level to Nostoc or Calothrix. Four isolates were identified at species level. As these cyanobacteria were intended to be used as biofertilizer for rice, several physiological parameters were studied. Growth rates, nitrogenase, nitrate reductase and glutamate synthase activities were evaluated. This information will be valuable to select strains to be used as inoculant for ricefields.

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“…Likewise, the generation of knowledge favors agricultural interventions based on an ecological vision of the production systems (75) . The biotechnological developments contribute to biological control programs for insect pests and diseases, as well as to nutritional management programs for forestry, agricultural and forage crops (76) . The rhizosphere and soil microbial communities, as well as the endophyte microorganisms and plant symbionts, collectively called microbiome, play an essential role in maintaining soil and plant health, and therefore the health of the global ecosystem.…”

Section: Inia's Contributions To Plant Health and Environmental Healthmentioning

confidence: 99%

One health: considerations in the International Year of Plant Health

Altier

Abreo

2020

Agrociencia

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The concept of “one health” is gaining strength at a global scale. The term summarizes a notion that has been known for more than a century: human and animal health are interdependent and linked to the ecosystems in which they coexist. In the International Year of Plant Health (2020), it is crucial that the agricultural sciences call attention for the need to consider the health of plants and their microbiome as a whole within the “one health” concept, closely linked to the health of the soil, the environment, animals and humans. In the context of agroecological production based on the sustainable management of natural resources, integrated pest management has been promoted to reduce the use of pesticides and mitigate negative effects on human and environmental health. The combination of genetic resistance with cultural and biological control have contributed to achieving this goal. The management of the microbiome offers also the possibility of addressing plant nutritional issues and the continuum between environmental, plant, animal and human health. With its strategic plan and long-term vision, INIA plays an essential role in the generation of knowledge and technologies, acting in accordance to the one health approach.

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Root Nodule and Rhizosphere Bacteria for Forage Legume Growth Promotion and Disease Management

Cited by 8 publications

References 65 publications

Selection of Competitive and Efficient Rhizobia Strains for White Clover

Selection of Competitive and Efficient Rhizobia Strains for White Clover

Potential cyanobacterial inoculants for rice described from a polyphasic approach

One health: considerations in the International Year of Plant Health

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