Root-preferential mRNA accumulation in Zea mays

Held, Bruce Marvin

doi:10.31274/rtd-180813-3186

Cited by 3 publications

(45 citation statements)

References 95 publications

(145 reference statements)

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“…These data are consistent with the ZRP2 mRNA accumulation pattern in which high levels of the mRNA were detected in the root except at the tip where it was undetectable (Held, 1993). In the mature plants, ZRP2 protein was detected at a high level in roots and stems but not detected in other organs.…”

Section: Discussionsupporting

confidence: 79%

“…These data support the hypothesis that ZRP2 gene is indeed expressed and functions in the roots and stems of maize. Comparison of western analysis data with in vivo transcription studies conducted previously (Held, 1993) proteins (John and Keller, 1995;Parsey et al, 1994;Kieliszewsky et al, 1990). For example, John and Keller described a proline rich protein from cotton with an estimated size of 21 kDa and an observed size of 55 kDa on SDS-PAGE.…”

Section: Discussionmentioning

confidence: 99%

“…These COMTs would be expected to be localized principally in the xylem tissue since COMTs are involved lignin biosynthesis. In contrast, ZRP4 mRNA levels accumulate to the highest level in the endodermis and at lower levels in the exodermis during the time when these regions are in the process of forming the Casparian strip (Held et al, 1993). A major 0-methylated plant metabolite known to accumulate specifically in the endodermis and exodermis at the time of deposition of Casparian strips is suberin .…”

Section: O-methyl Transferasesmentioning

confidence: 97%

“…The RNA fragments associated with the ZRP2 band has been previously observed and may indicate either in vitro or in vivo instability of this mRNA (Held, 1993).…”

Section: Effect Of Aba On Zrpl Expressionmentioning

confidence: 99%

“…mRNA is more abundant in etiolated seedlings than in light grown seedlings (Held, 1993). In this study, the effect of exogenous application of growth regulators was studied.…”

Section: Effect Of Aba On Zrpl Expressionmentioning

confidence: 99%

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Characterization of the expression of two maize root-preferential genes at the protein level

Moragoda

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This manuscript has been reproduced from the microfilm master. TJMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter &ce, \^e others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedtbrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: O-methyl Transferasesmentioning

confidence: 97%

“…The RNA fragments associated with the ZRP2 band has been previously observed and may indicate either in vitro or in vivo instability of this mRNA (Held, 1993).…”

Section: Effect Of Aba On Zrpl Expressionmentioning

confidence: 99%

“…mRNA is more abundant in etiolated seedlings than in light grown seedlings (Held, 1993). In this study, the effect of exogenous application of growth regulators was studied.…”

Section: Effect Of Aba On Zrpl Expressionmentioning

confidence: 99%

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Characterization of the expression of two maize root-preferential genes at the protein level

Moragoda

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Held¹,

John

Wang

et al. 1997

Plant Molecular Biology

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A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The function of the putative protein remains to be elucidated. The ZRP2 mRNA accumulates to the highest levels in young roots, and is also present in mature roots and stems of maize. Further analysis of young roots indicates that the lowest level of ZRP2 mRNA is near the root tip, with relatively high levels throughout the remainder of the root. In situ hybridization reveals that ZRP2 mRNA accumulates predominantly in the cortical parenchyma cells of the root. In vitro nuclear run-on transcription experiments indicate a dramatically higher level of zrp2 gene transcription in 3-day old roots than in 5-day old leaves. A zrp2 genomic clone, which includes the transcribed region and 4.7 kb of upstream sequence, was isolated and characterized.

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Protein-binding sequences that may regulate organ-preferential transcription of zrp2, a Zea mays root-preferential gene

Tirimanne

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In this study, D A-protein interactions of a one kilobase region proximal to the transcription start site of the zrp2 (Zea mays root-preferential 2) gene promoter was analyzed. The intent of this research was to identify regions of the promoter and D Aprotein interactions, that are responsible for root-preferential transcription. In vitro run-on transcription assays had re ealed dramatically higher levels of transcription in 3-d-old roots than in 5-d-old leaves (Held, 1993). Interactions between regions of the zrp2 promoter and nuclear protein extracts from 3-d-old roots and 5-d-old leaves of maize were analyzed by gel mobility-shift assays. Two D A-protein complexes with each protein extract were observed with the-4 72 to-180 region of the promoter. This region showed 72% nucleotide identity with a 159 bp portion of the first intron from an a-tubulin gene that is expressed preferentially in the roots of maize (Montoliu et al., 1989). AD Aprotein complex with high electrophoretic mobility (Z2BP1) was observed with both root and leaf nuclear protein extracts. Complexes Z2BP2 and Z2BP3 were preferentially formed with root and leaf nuclear protein extracts respecti ely. ubsequently, the proteinbinding region was delimited to a 73 bp (-330 to-258) region, which contained several repeats of two sequence motifs consisting only of As and Ts. The effect of the proteinbinding sequence on transcription of the 1 kbp and the 4.7 kbp promoter fragments (fused to the GU reporter gene) were analyzed by particle bombardment-mediated transient assays in roots and lea es of maize. The transcriptional activities of promoters, with the protein-binding region deleted were compared with their respecti e intact promoters. In roots the deletion resulted in 5-fold-less transcriptional activity with the 1 kbp promoter fragment, while the 4.7 kbp promoter showed no significant decrease. In lea es no ignificant decrease was observed with either 1 kbp or 4.7 kbp promoters. We conclude that this protein-binding region is important in enhancing the le el of transcriptional acti ity in roots.

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Root-preferential mRNA accumulation in Zea mays

Cited by 3 publications

References 95 publications

Characterization of the expression of two maize root-preferential genes at the protein level

Characterization of the expression of two maize root-preferential genes at the protein level

Untitled

Protein-binding sequences that may regulate organ-preferential transcription of zrp2, a Zea mays root-preferential gene

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