Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

doi:10.14258/turczaninowia.21.1.13

turczaninowia

2018

DOI: 10.14258/turczaninowia.21.1.13

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Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Abstract: Ключевые слова: адаптация ex vitro, гидропонная установка, индолил-3-масляная кислота, укоренение ex vitro, Rhododedron.Аннотация. Укоренение и адаптация являются лимитирующими стадиями для клонального микроразмно-жения растений, особенно у древесных. Представленное исследование направлено на изучение влияния раз-личных обработок индолил-3-маслянной кислотой (ИМК) на укоренение и адаптацию микропобегов шести морозостойких генотипов рододендронов в условиях in vitro и ex vitro. Испытывали два метода применения … Show more

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Cited by 9 publications

References 14 publications

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Challenges and insights in the acclimatization step of micropropagated woody plants

Grzelak,

Pacholczak,

Nowakowska

2024

Plant Cell Tiss Organ Cult

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Acclimatization is the last step of plants in vitro propagation while it comes to alter the environment conditions from tailor for plant requirements, sterile laboratory to external ex vitro conditions. Consequently, during acclimatization it may reach to plants mortality due to abiotic and biotic stresses occurring in external environment (high temperature, light intensity, low air relative humidity and the presence of microorganisms and pathogens in soil). Micropropagated plants develop alter organs on morphological, anatomical or biochemical levels which must be correct during acclimatization to improve adaptation of plants to ex vitro conditions. Therefore, there is a need to create acclimatization protocols to improve the effectiveness of this vegetative propagation method. So far there were formulated facilities as pre-acclimation, in vitro acclimatization, water cultures, CO2 enrichment, application of biostimulators and plant growth regulators. In following research we focused on describing previous achievements about the changes occurring in woody plants by micropropagation and summary of achievements in acclimatization of micropropagated woody plants. This article summary the current state of knowledge on acclimatization for various range of woody species by anatomical, physiological and biochemical levels. There are described ways to increase the survival of acclimatized plants and minimize acclimatization stress it also collects substances used so far to enhance plant growth during acclimatization.

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Challenges and insights in the acclimatization step of micropropagated woody plants

Grzelak,

Pacholczak,

Nowakowska

2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

In vitro and ex vitro rooting of Spiraea betulifolia subsp. aemiliana (Rosaceae), an ornamental shrub

Muraseva

Kostikova

2020

BIO Web Conf.

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Two methods of rhizogenesis – in vitro and ex vitro of Spiraea betulifolia subsp. aemiliana (C.K. Schneid.) H. Hara microshoots have been compared. Pulse treatment of microshoots with aqueous solutions of 4% “Heteroauxin” or 2% succinic acid (ex vitro rooting) did not effective -rooting frequency ranged from 3 to 19%. It was established that the in vitro rooting on nutrient media supplemented with auxins was a more effective technique, providing a high percentage of rooted microshoots. The use of half- strength MS medium supplemented with 0.1 μM indolyl-3-butyric acid (rooting frequency 8 8%, root number 3.5 ± 0.3 per plantlet) was found to be the most efective for in vitro rooting. The in vitro rooted regenerated plantlets were successfully acclimatized with 55% of survival rate.

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Collection of rare and endangered plant species in the meristem bank of the RAS Main Botanical Garden

Molkanova¹,

Gorbunov

Shirnina³

et al. 2021

E3S Web Conf.

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Results of many research years on in vitro formation and conservation of genetic plants' bank of rare and endangered species in the Main Botanical Garden of the Russian Academy of Sciences. Peculiarities of plants' cultivation and conservation in vitro related to different families were revealed. The main methodological aspects at the stages of obtaining axenic culture, micropropagation and long-term deposition are reflected. The main criterion in choosing the optimal explant type for both taxa introduction into the culture in vitro and for long-term reproduction and conservation is the plants’ life form. The nutrient media formulations and cultivation factors for slow explants’ growth of the studied crops and conservation of their viability were optimized. It was revealed that when selecting optimal conditions of cultivation and conservation it is necessary to consider the biological features of the taxon.

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Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Cited by 9 publications

References 14 publications

Challenges and insights in the acclimatization step of micropropagated woody plants

Challenges and insights in the acclimatization step of micropropagated woody plants

In vitro and ex vitro rooting of Spiraea betulifolia subsp. aemiliana (Rosaceae), an ornamental shrub

Collection of rare and endangered plant species in the meristem bank of the RAS Main Botanical Garden

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