ROS-dependent HMGB1 secretion upregulates IL-8 in upper airway epithelial cells under hypoxic condition

Min, Hyun Jin; Kim, J. H.; Yoo, Jihye; Oh, Jaewon; Kim, K. S.; Yoon, Joo Heon; Kim, C. H.

doi:10.1038/mi.2016.82

Cited by 44 publications

(43 citation statements)

References 34 publications

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“…IL-8 is a neutrophilic chemokine that is freely present in nasal lavage fluid and is a known associate of other DAMP molecules in chronic rhinosinusitis [19,20]. In this experiment (as in other reports), IL-8 was readily detected in all nasal lavage fluids; and although one particular study has demonstrated that in lower airways, baseline CXCL8 mRNA levels of sputum measured in swimmers exceeded those of controls [12].…”

Section: Discussionsupporting

confidence: 68%

“…2A, 2B). In measuring IL-8 (which was detected and is important in upper airway inflammatory pathways) [20], levels present before (288.94 ± 161.37 pg/mL) and after (319.70 ± 338.49 pg/mL) exercise were similar (Fig. 2C).…”

Section: Hsp27 and Hsp70 In Nasal Lavage Fluid Of Nonathletes (Pre-anmentioning

confidence: 80%

“…As subjects were supine position, some amount of instilled normal saline was got out of the posterior nasal airway, and some was entered to middle meatus, leaving 2.5 ml of nasal secretions by suction prior to intranasal manipulation. NAL fluid collection was performed following our previous protocols [19][20][21]. The secretions were immediately centrifuged to remove mucus and stored (-70°C) until needed.…”

Section: Nasal Lavage Fluid Collectionmentioning

confidence: 99%

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Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study

Min

Kang

et al. 2020

Int. J. Med. Sci.

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Purpose: Exercise modifies airway immune responses and susceptibility to infection. We investigated the effects of exercise on two HSPs (heat shock proteins), quantifying expression levels in nasal mucosa of both professional competitive athletes and non-athletes for comparison. Method: We used western blot technique to determine expression levels of HSPs in primary human nasal epithelial cells (HNECs). Nasal lavage (NAL) fluids were collected from 12 male professional volley ball players and 6 healthy males pre-submaximal exercise (running for 30 min at 70-80% of maximal heart rate) and post-submaximal exercise. Expression levels of HSP27, HSP70, Interleukin (IL)-8, and Tumor necrosis factor (TNF)-α in NAL fluids were quantified by enzyme-linked immunosorbent assay (ELISA), and difference of the level between pre-submaximal exercise and post-submaximal exercise was statistically analyzed. Antibacterial assay using Staphylococcus aureus was performed to assess the immunological role of HSPs in NAL fluids. Results:. In non-athlete controls, HSP27, HSP70, and IL-8 were unchanged after exercise. In the professional athletes, HSP70 expression was declined significantly (p<0.05), but HSP27 was not significantly changed. IL-8 and TNF-α did not show significant difference, either. By antibacterial assay, it was found that the number of active bacterial populations were influenced by the presence or absence of HSP27 and HSP70 in NAL fluids. Conclusion: HSP27 and HSP70 were present in NAL fluids of enrolled subjects, and the effect of exercise on the level HSPs was different between professional athletic competitors and non-athletes. As the number of active bacterial population was influenced by the presence or absence of nasal HSP27 and HSP70, we suggest that HSP27 and HSP70 may play immunological function in NAL fluids.

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Section: Discussionsupporting

confidence: 68%

Section: Hsp27 and Hsp70 In Nasal Lavage Fluid Of Nonathletes (Pre-anmentioning

confidence: 80%

Section: Nasal Lavage Fluid Collectionmentioning

confidence: 99%

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Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study

Min

Kang

et al. 2020

Int. J. Med. Sci.

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“…Above results confirmed that ROS and HMGB1 can regulate the p53 and PI3K/Akt pathways. Min et al ( 2017 ) found that hypoxia induced translocation of HMGB1 into the extracellular area and it was dependent on ROS produced by dual oxidase 2. This further proved that ROS can regulate the functions of HMGB1.…”

Section: Discussionmentioning

confidence: 99%

IL-17A Enhances Microglial Response to OGD by Regulating p53 and PI3K/Akt Pathways with Involvement of ROS/HMGB1

Zhang

Yang

et al. 2017

Front. Mol. Neurosci.

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Cerebral ischemia-reperfusion injury (IRI) has a complex pathogenesis, and interleukin-17 (IL-17) is a newly identified class of the cytokine family that plays an important role in ischemic inflammation. An oxygen-glucose deprivation (OGD) model showed that IL-17A expression was significantly up-regulated in microglial cells. After IL-17A siRNA transfection, the inhibition of proliferation, and the increased apoptosis in microglial cells, induced by OGD/reperfusion, was improved, and the elevation of Caspase-3, Caspase-8, Caspase-9, and poly ADP ribose polymerase (PARP) activities was inhibited. Mass spectrometry demonstrated that IL-17A functioned through a series of factors associated with oxidative stress and apoptosis and regulated Caspase-3 activity and apoptosis in microglial cells via the p53 and PI3K/Akt signaling pathways. IL-17A, HMGB1, and ROS were regulated mutually to exhibit a synergistic effect in the OGD model of microglial cells, but the down-regulation of IL-17A or HMGB1 expression did not completely inhibit the production of ROS. These findings demonstrated that ROS might be located upstream of IL-17A and HMGB1 so that ROS can regulate HMGB1/IL-17A expression to affect the p53 and PI3K/Akt signaling pathways and therefore promote the occurrence of apoptosis in microglial cells. These findings provide a novel evidence for the role of IL-17A in ischemic cerebral diseases.

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“…Recently, it has been reported that the alarmin HMGB1 mediates its many activities via an ROS-dependent mechanism. 45 HMGB1 is a known target of such redox reaction molecules, and it contains two redox-sensitive cysteine moieties at positions C23 and C45, the redox states of which greatly affect HMGB1 function. 46 When these cysteine residues are presented in reduced thiol form, HMGB1's prodamage signaling is minimal.…”

Section: Discussionmentioning

confidence: 99%

High mobility group box 1 and markers of oxidative stress in human cord blood

D’Angelo

Granese

Marseglia

et al. 2019

Pediatrics International

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Background Parturition induces considerable oxidative stress and many inflammatory mediators, such as high mobility group box 1 (HMGB1), are involved from the beginning of the pregnancy to birth. The aim of the present study was to evaluate serum cord blood concentration of diacron‐reactive oxygen metabolites (d‐ROM), biological antioxidant potential (BAP), and HMGB1 to investigate the perinatal oxidative status of neonates and correlation with mode of delivery, as well as the influence of labor. Methods The subjects consisted of 214 neonates delivered at University Hospital “G. Martino”, Messina, in a 6 months period. Venous blood samples were collected from the umbilical cord after cord separation. Results Umbilical cord venous blood HMGB1 was significantly higher in the spontaneous vaginal delivery (SVD) group than in the elective or emergency cesarean section (CS) group (P = 0.018). Regarding labor, there was no significant difference in HMGB1 concentration in umbilical vein blood between the spontaneous and induced labor groups (P = 0.250). Furthermore, d‐ROM was significantly different between the SVD group and the elective or emergency CS group (P = 0.044). BAP concentration, however, was not significantly different, not even with regard to mode of labor. Conclusion Oxidation is higher in newborns delivered by SVD than in those delivered by CS, and HMGB1 may be involved in the mechanisms of birth, and responsible for decidual modifications that lead to birth.

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ROS-dependent HMGB1 secretion upregulates IL-8 in upper airway epithelial cells under hypoxic condition

Cited by 44 publications

References 34 publications

Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study

Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study

IL-17A Enhances Microglial Response to OGD by Regulating p53 and PI3K/Akt Pathways with Involvement of ROS/HMGB1

High mobility group box 1 and markers of oxidative stress in human cord blood

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