ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

Abstract

Magnetic nanoparticles have been used in diverse pharmaceutical applications because they can potentially be used to target specific sites. In the present work, a new type of nanocomposites is designed as a carrier of controlled bioactive agent delivery. Methods: Amine-functionalized magnetic nanoparticles (amine-MNPs) are coupled with carboxymethyl chitosan (CMC) to generate the nanocomposites, namely MNPs-CMC, which can be further loaded with doxorubicin (DOX) to produce MNPs-CMC-DOX. The generated nanocomposites are characterized by using various techniques (including FTIR, 1 H-NMR, DSC, TGA, SEM, TEM and XRD). In vitro drug release studies are conducted in PBS with different pH values (1.2 and 6.8) at different temperatures (25°C and 37°C). The toxicity of the nanocomposites is tested in MCF-7 and 3T3 cells. The ROS-generating capacity of the nanocomposites is determined in treated cells using 2',7'-dichlorodihydrofluorescein diacetate. Results: The structures of MNPs, CMC, and nanocomposites are confirmed by FTIR, XRD, and 1 H-NMR data reveals the formation of CMC from chitosan (CS). The size of MNPs is estimated by TEM to be around 25 nm. After conjugation with CMC, the size of the nanocomposites increases to 46-57 nm. Based on the release profiles of MNPs-CMC-DOX, our nanocomposites are pH-responsive. In addition, our nanocomposites show reactive oxygen species (ROS)-generating capacity and cell type-dependent toxicity. Conclusion: Our nanocomposites show high potential for use in bioactive agent delivery. Along with their ROS-generating capacity, they warrant further development as pH-responsive carriers for therapeutic applications.

[Retracted] Effect of Ruanjian Xiaoying Granules on Hashimoto Rats with Depression of Liver and Deficiency of Spleen and Effect on Intestinal Microflora

“…At class level, the number of clostridium in the model group was higher than that in the normal group, while the number of clostridium in the traditional Chinese medicine group was lower. Studies have shown that the colonization of clostridium intestinalis can effectively induce the production of IL-17 and IL-22 by CD4 helper T cells (namely 17 cells) in the lamina propria of the intestinal mucosa, and the more 17 cells detected in the lamina propria of the intestinal mucosa, the more clostridium colonized in the intestinal mucosa [17][18][19][20]. However, the intestinal tract with fewer 17 cells in the lamina propria lacks clostridium colonization.…”

Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and Their In Vivo Multiple Biological Applications

“…In addition to being a unique technique for transporting zinc, zinc oxide nanoparticles (ZnO-NPs) have crucial implications in the treatment of a range of disorders, including diabetes [ 13 ]. Zinc supplementation has been demonstrated to have an ameliorative impact in preclinical investigations [ 13 , 14 ]. As a result, the development of a zinc-based medication for the treatment of diabetes and its complications might be promising [ 15 ].…”

Magnetic chitosan stabilized Cu(II)-tetrazole complex: an effective nanocatalyst for the synthesis of 3-imino-2-phenylisoindolin-1-one derivatives under ultrasound irradiation

“…Today, biopolymers are used in the different fields 6 , 47 , 52 – 56 . Among biopolymers, chitosan is an N -deacetylated type of chitin, found in nature, such as in Crabs, Shrimp, squid, etc.…”

“…1 ). Chitosan (CS) is an abundant, biodegradable, hydrophilic, and natural polysaccharide, which affords desirable structural features for mechanical and chemical modifications 6 , 47 , 52 . It is an ideal support for the immobilization of metal nanoparticles due to its high stability, high surface area and low cost.…”