ROS Suppression by Egg White Hydrolysate in DOCA-Salt Rats—An Alternative Tool against Vascular Dysfunction in Severe Hypertension

Abstract

This study aimed to evaluate the potential for lowering blood pressure and beneficial effects on mesenteric resistance arteries (MRA) and conductance vessels (aorta) produced by dietary supplementation of an egg white hydrolysate (EWH) in rats with severe hypertension induced by deoxycorticosterone plus salt treatment (DOCA-salt), as well as the underlying mechanisms involved. The DOCA-salt model presented higher blood pressure, which was significantly reduced by EWH. The impaired acetylcholine-induced relaxation and eNOS expression observed in MRA and aorta from DOCA-salt rats was ameliorated by EWH. This effect on vessels (MRA and aorta) was related to the antioxidant effect of EWH, since hydrolysate intake prevented the NF-κB/TNFα inflammatory pathway and NADPH oxidase-induced reactive oxygen species (ROS) generation, as well as the mitochondrial source of ROS in MRA. At the plasma level, EWH blocked the higher ROS and MDA generation by DOCA-salt treatment, without altering the antioxidant marker. In conclusion, EWH demonstrated an antihypertensive effect in a model of severe hypertension. This effect could be related to its endothelium-dependent vasodilator properties mediated by an ameliorated vessel’s redox imbalance and inflammatory state.

Egg white hydrolysate protects white adipose tissue against metabolic insult in deoxycorticosterone acetate-salt rats

“…Moreover, it is known that specific amino acids and their position in the sequence are essential for bioactivity (34,35) . The antihypertensive effect produced after EWH administration in the DOCA-salt model was a 32 % reduction in SBP, corroborating a previous report using this hydrolysate (20) . Other bioactive compounds derived from foods have also demonstrated antihypertensive effects in hypertensive experimental models, specifically in DOCA-salt hypertensive animals.…”

“…Bioactive compounds from animal sources, particularly bioactive peptides, have been extensively studied. In this context, an egg white hydrolysate (EWH) produced by treatment with pepsin for 8 hours has demonstrated antioxidant, anti-inflammatory and/or antihypertensive properties in several experimental models such as Zucker obesity (17) , spontaneously hypertensive rat (18) , metabolic syndrome (19) and deoxycorticosterone acetate (DOCA)-salt hypertension (20) related to the action of bioactive peptides which are included on EWH.…”

Preparation, Biological Activities, and Potential Applications of Hen Egg-Derived Peptides: A Review

“…And Aleksandra et al (2014) reported that EYPs exerted a strong ACE inhibitory activity (IC 50 of 9.4 µg/mL), which has been attributed mainly to the glutamic acid residue at the C-terminal positions [95]. Moreover, it has been found that oxidative stress is related to vascular damage in hypertensive patients, due to the degradation of excessive reactive oxygen species (ROS) and nitric oxide (NO) [124]. Zhang et al (2020) reported that LAPYK, identified from EWPPs, had an ABTS scavenging IC 50 of 5.29 ± 0.24 µM and ORAC of 1.50 ± 0.04 µM TE/µM, and found that LAPYK has potential to exhibit antihypertensive activity [118].…”

Sprague-Dawley Rats Induced by Uninephrectomy, DOCA Injection, and Sodium Chloride: A Suitable Model for Chronic Hypertension with Cardiac Hypertrophy

“…We used this model to demonstrate that it induces severe hypertension and impacts blood volume; thus, it is useful for studies on resistant hypertension (Lee et al 2015;Abreu et al 2022). It is widely known that salt sensitivity plays a role in the onset of essential hypertension in humans (Grillo et al 2019).…”