Rosa spp. (Roses): In Vitro Culture, Micropropagation, and the Production of Secondary Products

Short, K. C.; Roberts, A. V.

doi:10.1007/978-3-642-84071-5_23

Cited by 24 publications

(18 citation statements)

References 52 publications

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“…It is a long term storage procedure used to preserve germplasm using minimum space and maintenance (Short and Roberts, 1991). It has been applied to a wide range of plant species and tissues (Reed, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Mubbarakh¹,

Izhar²,

Rajasegar³

et al. 2014

Emir. J. Food Agric

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This study was carried out to evaluate the encapsulation-dehydration technique on IFEs of Rosa hybrida L. cv. Helmut Schmidt. The survival of IFEs was first assessed based on the effects of four sucrose concentrations (0, 0.25, 0.5 and 0.75 M) at different durations (0, 24 and 48 hours). The IFEs were then encapsulated and osmoprotected on shaker at 110 rpm with various sucrose concentrations (0, 0.1, 0.5 and 0.75 M). Subsequently, encapsulated IFEs were dehydrated under laminar air flow during three different time periods (0, 3 and 6 hours) on oven sterilized 50 g silica gel. The encapsulated IFEs were plunged into liquid nitrogen for a minimum duration of 24 hour. Encapsulated IFEs were thawed at 40ºC for 90 seconds and cultured on fullstrength MS medium supplemented with 3% sucrose for a week in dark, a week in semi-light and an additional 1 week under full light exposure. IFEs were then evaluated for survival by 2,3,5-Triphenyltetrazolium Chloride assay at 490 nm. The best conditions for the encapsulation-dehydration of IFEs of Rosa hybrida L. cv. Helmut Schmidt was obtained when IFEs were precultured in 0.25 M sucrose for 24 hours, osmoprotected in 0.75 M sucrose and dehydrated for 3 hours. Histological analysis showed cryopreserved IFEs with intensely stained nucleus but with severely damaged membranes.

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Section: Discussionmentioning

confidence: 99%

“…It contains more than 100 species and comes in variety of colours and fragrance (Lynch, 2002). Roses have well significant commercial value to the horticulture society (Short and Roberts, 1991). Therefore it is a must for rose breeders to maintain their cultivars before they face extinction (Luza and Polito, 1988).…”

Section: Introductionmentioning

confidence: 99%

Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Mubbarakh¹,

Izhar²,

Rajasegar³

et al. 2014

Emir. J. Food Agric

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“…The factors that influence micropropagation of medicinal and aromatic plants have been reviewed by Bhagyalakshmi and Singh (1988), Hu and Wang (1983), Hussey (1980Hussey ( , 1983, Murashige (1977a), Rout et al (2000), Short & Roberts (1991).…”

Section: Biotechnological Tools In Conservationmentioning

confidence: 99%

Ethnomedicinal Plants of India with Special Reference to an Indo-Burma Hotspot Region: An overview

Kumar

Lalramnghinglova

2011

Ethnobot. Res. App.

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“…Numerous factors are reported to influence the success of in-vitro propagation of different medicinal plants 9,[13][14][15] . .…”

Section: In-vitro Regenerationmentioning

confidence: 99%

Role of biotechnology in medicinal plants

Tripathi

2005

Trop. J. Pharm Res

111

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Rosa spp. (Roses): In Vitro Culture, Micropropagation, and the Production of Secondary Products

Cited by 24 publications

References 52 publications

Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Ethnomedicinal Plants of India with Special Reference to an Indo-Burma Hotspot Region: An overview

Role of biotechnology in medicinal plants

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