Rose Germplasm Analysis with RAPD Markers

Abstract

<jats:p>The genus <jats:italic>Rosa</jats:italic> consists of more than 100 species classified into four subgenera, <jats:italic>Eurosa</jats:italic>, <jats:italic>Platyrhodon</jats:italic>, <jats:italic>Hesperhodos,</jats:italic> and <jats:italic>Hulthemia,</jats:italic> and distributed widely throughout the northern hemisphere. The subgenus <jats:italic>Eurosa</jats:italic> includes 11 sections. The other subgenera are monotypic. One hundred and nineteen accessions and 213 markers of 36 rose species that include eight sections of the subgenus <jats:italic>Eurosa</jats:italic> and one species each from the subgenera <jats:italic>Hesperhodos</jats:italic> and <jats:italic>Platyrhodon</jats:italic> were used to calculate a similarity matrix, which was clustered with the unweighted pair group method using arithmetic means (UPGMA). The RAPD markers distinguished between all the rose accessions, and species grouped into their respective sections. Therefore, classification of <jats:italic>Rosa</jats:italic> using RAPD data generally supports traditional classification. The Asian rose sections (<jats:italic>Laevigatae</jats:italic>, <jats:italic>Banksianae</jats:italic>, <jats:italic>Bracteatae</jats:italic>, <jats:italic>Pimpinellifoliae</jats:italic>, <jats:italic>Chinenses,</jats:italic> and <jats:italic>Synstylae</jats:italic>) were consistently separated from the primarily North American sections (<jats:italic>Cassiorhodon</jats:italic> and <jats:italic>Carolinae</jats:italic>). The <jats:italic>Cassiorhodon</jats:italic> and <jats:italic>Carolinae</jats:italic> sections were grouped together with the subgenera <jats:italic>Hesperhodos</jats:italic> and <jats:italic>Platyrhodon</jats:italic>. Both subgenera separated out at the same level as sections within the subgenus <jats:italic>Eurosa</jats:italic>, suggesting that they are more appropriately classified as sections within the subgenus <jats:italic>Eurosa</jats:italic>. Sections <jats:italic>Cassiorhodon</jats:italic> and <jats:italic>Carolinae</jats:italic> overlapped, and are probably best grouped as one section as previously suggested.</jats:p>

AFLP markers as a tool to reconstruct complex relationships: A case study in Rosa (Rosaceae)

“…Pimpinellifoliae , this places R. persica and R. roxburghii within the subgenus Rosa , dismissing their subgeneric status. The incorporation of both subgenera in subgenus Rosa is in line with previous results on RAPDs (Jan et al, 1999; but only R. roxburghii was included), and on matK (Matsumoto et al, 1998), ITS (Wu et al, 2001b; Wissemann and Ritz, 2005), atpB‐rbcL intergenic spacer (Wissemann and Ritz, 2005; but only for R. roxburghii ), and trnL ‐ F / psbA ‐ trnH spacer sequences (Bruneau et al, 2007). The close relationship of R. persica with sect.…”

“…2 and 3). This position within Cinnamomeae is supported by RAPD data (Jan et al, 1999), AFLP and morphology data (Joly and Bruneau, 2007), and sequence data on ITS (Wissemann and Ritz, 2005), atpB‐rbcL (Wissemann and Ritz, 2005), GAPDH Joly et al, 2006), and trnL‐F / psbA‐trnH (Bruneau et al, 2007). Therefore, our results corroborate the conclusion of these authors that sect.…”

“…The sect. Synstylae is generally considered to be monophyletic, a view that is corroborated by data on RAPDs (Millan et al, 1996; Jan et al, 1999), matK (Wu et al, 2000), and ITS sequences (Matsumoto et al, 2000; Wu et al, 2001b). In our results, the section appears as a polyphyletic group including clades 3a, 3b, and a part of 2b, but the distinction between these clades is only weakly supported.…”

“…In a study on wild accessions, Millan et al (1996) showed a clustering largely according to the sectional affinities. The latter was also found by Jan et al (1999). Wu et al (2001a) used RAPDs to study the relationships within sect.…”

Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers

“…Within the rootstocks, the distance between varieties originating from different species is relatively high and varieties within a species are well clustered, although these clusters do contain some varieties belonging to different species. Distinct clustering of accessions belonging to one Rosa species was also observed with RAPD markers (Debener et al 1996;Millan et al 1996;Reynders-Aloisi and Bollereau 1996;Jan et al 1999;Martin et al 2001). The molecular differentiation contrasts to the fact that it is difficult to distinguish all those varieties using morphological measurements.…”