Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).

Abstract

CD2, also known as LFA-2, T11, and the E rosette receptor, is a T lymphocyte surface protein functionally important in adhesion to target cells and T cell triggering. LFA-3 is a widely distributed cell surface protein that functions in adhesion on target cells. We find that LFA-3 is expressed on human E, and that CD2 is a receptor for LFA-3 that mediates T cell adhesion to human E. Pretreatment of T lymphocytes with CD2 mAb or of E with LFA-3 mAb inhibits rosetting. Purified CD2 molecules bind to human E and inhibit rosetting. 125I-CD2 binding to E is inhibited by LFA-3 mAb; reciprocally, binding of LFA-3 mAb to human E is inhibited by pretreatment with purified CD2. Higher concentrations of CD2 aggregate human E; aggregation is inhibited by mAb to LFA-3.

Expression of the T-cell surface molecule CD2 and an epitope-loss CD2 mutant to define the role of lymphocyte function-associated antigen 3 (LFA-3) in T-cell activation.

“…The CD2 molecule has been shown to interact with LFA-3 on the human erythrocyte (4,6) or with the sheep homologue of LFA-3 (33). The murine hybridomas expressing the human CD2 molecule were found to produce IL-2 in response to stimulation with sheep RBC in the presence of the anti-CD2 mAb 9-1 (Table 3, Exp.…”

“…The T-cell erythrocyte receptor, or CD2 (T11, Leu 5, lymphocyte function-associated antigen 2) molecule, is a 50-kDa protein that mediates T-cell adhesion to sheep (1,2) and human (3)(4)(5)(6) erythrocytes. Certain monoclonal antibodies (mAbs) directed against CD2 inhibit T-cell rosette formation (7)(8)(9)(10), as well as cytolytic T-cell function (11,12) and T-cell proliferation (11)(12)(13)(14).…”

Glycolipid reanchoring of T-lymphocyte surface antigen CD8 using the 3' end sequence of decay-accelerating factor's mRNA.

“…1). In human cells, these proteins include decayaccelerating factor (DAF) of complement (2,3), erythrocyte acetylcholinesterase (4), placental and hepatic alkaline phosphatase (5), and lymphocyte function-associated antigen 3 (LFA-3) (6). While the physiological relevance of this mechanism of membrane linkage is not yet clear, glycolipidanchored proteins exhibit special properties.…”

Exon-intron organization and sequence comparison of human and murine T11 (CD2) genes.

“…§ Of note, the extracellular segment of the mature T11 protein is encoded by two exons, whereas almost the entire intracellular segment and 3'-untranslated region is encoded on a single exon. The 50-kDa T11 (CD2) surface glycoprotein plays a major role in T-lymphocyte function (1)(2)(3)(4)(5). Monoclonal antibodies directed against an epitope on the external segment of the human T11 molecule (T111) block T-lymphocyte activation, including that mediated through the T-cell receptor for antigen and major histocompatibility complex (Ti-T3) (6).…”