Rotating bed reactor packed with heterofunctional structured silica-supported lipase. Developing an effective system for the organic solvent and aqueous phase reactions

Kowalczykiewicz, Daria; Szymańska, Katarzyna; Gillner, Danuta; Jarzębski, Andrzej B.

doi:10.1016/j.micromeso.2020.110789

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…Enzymes can be immobilized by covalent or non-covalent (adsorption) interaction with support [28]. Lipases (especially lipase B from Candida antarctica), due to their specific structure, prefer hydrophobic carriers and non-covalent immobilization method [26,31]. In the presented study, MCFs were applied as carriers for lipase immobilization.…”

Section: Carriers Characterizationmentioning

confidence: 99%

“…Polymeric carrier of Novozym 435 features higher affinity to organic solvents, whereas silica to water. However, despite the good affinity of Novozyme 435 for organic solvents, its stability is rather poor [24][25][26][27].…”

Section: Biocatalysts Characterizationmentioning

confidence: 99%

“…The most popular hydrophobic carriers are organic polymers such as poly(methyl methacrylate) (PMMA) which constitutes the core of the Novozym 435 (the most popular commercial lipase-based biocatalyst). However, the major disadvantage of polymer carriers is their low stability in organic solvents, which was shown among others by Zhao, Jose and Kowalczykiewicz [24][25][26]. For this very reason the search for stable, hydrophobic carriers for lipase is still an important issue [27].…”

Section: Introductionmentioning

confidence: 99%

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Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

et al. 2021

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Dynamic kinetic resolution (DKR) is one of the most attractive methods for enantioselective synthesis. In the reported studies, lipase B from Candida antarctica (CALB) immobilized on siliceous mesoporous cellular foams (MCF) functionalized with different hydrophobic groups, and two ruthenium complexes with substituted cyclopentadienyl ligands were investigated as catalysts for the chemoenzymatic DKR of (rac)-1-phenylethanol, using Novozym 435 as a benchmark biocatalyst. Studies on the (rac)-1-phenylethanol transesterification reaction showed that CALB supported on MCFs grafted with methyl groups is a promising biocatalyst and isopropenyl acetate is a preferable acylation agent. Both Ru-complexes activated by K3PO4 or t-BuOK, proved to be effective catalysts of the racemization reaction. The final DKR experiments using all catalysts combinations singled out, gave 96% conversion, and (R)-1-phenylethyl acetate enantiomeric excess of 98% in 8 h using K3PO4 activator.

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Section: Carriers Characterizationmentioning

confidence: 99%

Section: Biocatalysts Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

et al. 2021

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“…However, to make full use of RBR potentials to improve reaction kinetics, we proposed to load the basket with larger catalytic pellets/beads, a few millimeters in size, but with a hierarchical pore structure, as in the M-monoliths (vide supra) (Szymańska et al, 2017). Such studies were carried out more recently, and the structured catalytic pellets did indeed enhance the reaction kinetics up to an order of magnitude, compared to the RBR packed with the most conventional particulate catalysts (Odrozek et al, 2017;Szymańska et al, 2017;Kowalczykiewicz et al, 2021). These studies are summarized in the following.…”

Section: Rotating Bed Reactors With Hierarchically Structured Catalytic Packingmentioning

confidence: 99%

“…Most recently, the performance of the same synthetic system has been tested in hydrolysis and esterification reactions carried out in either the aqueous phase or organic solvents using CALB lipase (Kowalczykiewicz et al, 2021). The enzyme was attached to the pre-functionalized pellets in an open-lid conformation, either by covalent bonding or by adsorption forces.…”

Section: Rotating Bed Reactors With Hierarchically Structured Catalytic Packingmentioning

confidence: 99%

Catalytic Functionalized Structured Monolithic Micro-/Mesoreactors: Engineering, Properties, and Performance in Flow Synthesis: An Overview and Guidelines

et al. 2021

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In this review article, we first discussed the development of silica monoliths with hierarchical macro-/mesopore structure and their potential figures of merit as continuous-flow micro-/mesoreactors of up to 30 ml working volume. Making use of the flow hindrance of different pore structures seen from the Darcy law perspective, we discriminated four structures of the monoliths (M1–M4). We then summarized the most important results, mainly from our studies of continuous-flow structured monolithic reactors and rotating bed reactors (RBRs) filled with structured pellets, activated with various catalytic entities and enzymes. The results show that an increase in the flow rate and thus velocity in reactors activated with more conventional catalytic sites has no or a minor positive effect on the apparent reaction rate. On the contrary, in those with the most open structure (M1) and functionalized with enzymes, it could increase by more than two orders of magnitude even at low overpressures. The production systems worked stably for at least 200 h. To conclude, the synthetic system made of the hierarchically structured monoliths, or RBRs filled with structured catalytic pellets, lay the foundation for a new platform for the high-yield production of a wide variety of specialty chemicals, even on a multikilogram scale, in a safe and sustained manner.

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Process optimization for enzymatic production of a valuable biomass-based ester from levulinic acid

Cambraia¹,

Barbosa²,

Soares³

et al. 2022

Bioprocess Biosyst Eng

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Rotating bed reactor packed with heterofunctional structured silica-supported lipase. Developing an effective system for the organic solvent and aqueous phase reactions

Cited by 15 publications

References 41 publications

Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

Catalytic Functionalized Structured Monolithic Micro-/Mesoreactors: Engineering, Properties, and Performance in Flow Synthesis: An Overview and Guidelines

Process optimization for enzymatic production of a valuable biomass-based ester from levulinic acid

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