Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Das, Dhrubajyoti; Lin, Cheng‐Wen; Kwon, Jae‐Sung; Chuang, Huey-Ru

doi:10.1016/j.bios.2022.114293

Cited by 8 publications

(5 citation statements)

References 68 publications

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“…Analysis of 300 images of blinking signals from 1 µm sized half-fluorescent half-gold functionalized Janus particles via a cross-correlation algorithm obtained a LOD of 48.2 pg/µL of E. coli gDNA in just 10 min, with a sample volume as low as 2 µL. In the follow-up article, the authors reported the detection of SARS-CoV-2 nsp-2 cDNA in a recombinant plasmid with a LOD of 70 ag/µL in 10 min using rotational diffusometry combined with LAMP [ 105 ]. It 10-fold higher sensitivity compared to the gold standard: real-time PCR.…”

Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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Seeking optimized infectious pathogen detection tools is of primary importance to lessen the spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid-based sensing techniques, loop-mediated isothermal amplification is a promising method, as it provides rapid, sensitive, and specific detection of microbial and viral pathogens and has enormous potential to transform current point-of-care molecular diagnostics. In this review, the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined. The numerous detection methods of LAMP-based biosensors are discussed in an end-point and real-time manner with ideal examples. We also summarize the trends in LAMP-on-a-chip modalities, such as classical microfluidic, paper-based, and digital LAMP, with their merits and limitations. Finally, we provide our opinion on the future improvement of on-chip LAMP methods. This review serves as an overview of recent breakthroughs in the LAMP approach and their potential for use in the diagnosis of existing and emerging diseases.

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Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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“…However, this measurement is a multi-step technique that relies on enzymes, complex machines and trained operational professionals, which may be incapable and not suitable for large-scale rapid and timely screening, such as in the food industry and cold-chain foods safety monitoring, to some extent. Many researchers turn to isothermal techniques and portable real-time devices [ 25 ]. Their properties, independence of thermal cycling instruments, isothermal performance and point-of-need make it a great possibility to integrate these technologies into cold-chain transportation for on-site setting diagnostics, creating a cost-effective and time-efficient approach.…”

Section: The Current Status Of Detection Methods For Sars-cov-2mentioning

confidence: 99%

Needs, Challenges and Countermeasures of SARS-CoV-2 Surveillance in Cold-Chain Foods and Packaging to Prevent Possible COVID-19 Resurgence: A Perspective from Advanced Detections

Qiao

Xiao

Zhao

et al. 2022

Viruses

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The pandemic caused by SARS-CoV-2 has a huge impact on the global economy. SARS-CoV-2 could possibly and potentially be transmitted to humans through cold-chain foods and packaging (namely good-to-human), although it mainly depends on a human-to-human route. It is imperative to develop countermeasures to cope with the spread of viruses and fulfil effective surveillance of cold-chain foods and packaging. This review outlined SARS-CoV-2-related cold-chain food incidents and current methods for detecting SARS-CoV-2. Then the needs, challenges and practicable countermeasures for SARS-CoV-2 detection, specifically for cold-chain foods and packaging, were underlined. In fact, currently established detection methods for SARS-CoV-2 are mostly used for humans; thus, these may not be ideally applied to cold-chain foods directly. Therefore, it creates a need to develop novel methods and low-cost, automatic, mini-sized devices specifically for cold-chain foods and packaging. The review intended to draw people’s attention to the possible spread of SARS-CoV-2 with cold-chain foods and proposed perspectives for futuristic cold-chain foods monitoring during the pandemic.

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“…This sensor improved the linear range of 0.75-250 pg/mL, and the LOD to 0.37 pg/mL, which provides an alternative method to detect breast cancer tumor biomarkers [40]. Recently, Janus particles (JPs) driven by rotational Brown motion have been used as novel probes for the rapid detection of diseases based on enhanced nucleic acid amplification [41] and small protein biomarkers [42]. By detecting the microviscosity change through loop-mediated isothermal amplification (LAMP) in the presence of target cDNA, SARS-CoV-2 nsp2, an LOD down to 70 ag/µL is achieved in 10 min, showcasing 100-fold higher in sensitivity and 15-fold faster than conventional polymerase chain reaction (PCR).…”

Section: Ultra-sensitive Probesmentioning

confidence: 99%

Recent Trends and Innovations in Bead-Based Biosensors for Cancer Detection

Cheng,

Yang,

Wang

et al. 2024

Sensors

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Demand is strong for sensitive, reliable, and cost-effective diagnostic tools for cancer detection. Accordingly, bead-based biosensors have emerged in recent years as promising diagnostic platforms based on wide-ranging cancer biomarkers owing to the versatility, high sensitivity, and flexibility to perform the multiplexing of beads. This comprehensive review highlights recent trends and innovations in the development of bead-based biosensors for cancer-biomarker detection. We introduce various types of bead-based biosensors such as optical, electrochemical, and magnetic biosensors, along with their respective advantages and limitations. Moreover, the review summarizes the latest advancements, including fabrication techniques, signal-amplification strategies, and integration with microfluidics and nanotechnology. Additionally, the challenges and future perspectives in the field of bead-based biosensors for cancer-biomarker detection are discussed. Understanding these innovations in bead-based biosensors can greatly contribute to improvements in cancer diagnostics, thereby facilitating early detection and personalized treatments.

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Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Cited by 8 publications

References 68 publications

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

Needs, Challenges and Countermeasures of SARS-CoV-2 Surveillance in Cold-Chain Foods and Packaging to Prevent Possible COVID-19 Resurgence: A Perspective from Advanced Detections

Recent Trends and Innovations in Bead-Based Biosensors for Cancer Detection

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