Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Kang, Yuhui; Zhou, Hongshan; Jin, Wenjie

doi:10.3390/vetsci9050197

Cited by 2 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the opportunistic pathogen Rothia nasimurium was first in the PM2.5 samples at 13.6%, and it can infect animals such as dogs, pigs, ducks, rabbits, and geese. The bacterium was isolated from captive chickens and geese for the first time and was found to be multidrug resistant by drug sensitivity test and animal regression test, resulting in severe hair loss in chickens and geese [68,69]. Staphylococcus aureus (0.46%) is a kind of pathogen that can cause primary infections in humans or animals, leading to severe clinical conditions such as bacteremia and sepsis [70].…”

Section: Discussionmentioning

confidence: 99%

Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Qu,

Wang,

et al. 2024

Indoor Air

View full text Add to dashboard Cite

Particulate matter (PM) and the microorganisms of duck houses may have negative impacts on animal and human health. During 2021-2022, PM2.5 and PM10 inside and outside the duck house were sampled with a built-in air sampler in Tai’an City, Shandong Province, and the diversity and abundance of microorganisms within the PM were analyzed by macrogenomic and absolute sequence analysis. The results showed that PM2.5 and PM10 concentrations in the house and at downwind points exceeded the short-term (24 h) guideline of the global air quality guidelines (AQG). Macrogenome sequencing showed that the microbial composition of the PM2.5 samples was dominated by bacteria (exceed 85%); a total of 1316 bacterial genera and 110 fungal genera were identified in PM2.5 samples from duck house 1 in winter, which were much higher than the results of amplicon sequencing method reported before, and relatively high levels of the pathogenic bacteria (Coccidioides immitis, etc.) and the conditionally pathogenic bacterium (Rothia nasimurium) were identified at the species level. Absolute quantitative sequencing detected conditionally pathogenic bacteria and allergens at high levels in PM10 samples: Corynebacterium (5.6×107 copies/g), Aerococcus (9.9×106 copies/g), Alternaria (3.3×106 copies/g), and Aspergillus (8.3×105 copies/g). Moreover, Corynebacterium was the highest content of PM10 in summer and PM2.5 samples in winter, and its pathogenicity and potential threat should be noted. The diversity and relative abundance of microorganisms were similar in the duck house and at the downwind point. The results showed that the microorganisms in the house environment have a greater influence on the air environment around the downwind point and may pose a public health risk to the staff and the surrounding area.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Qu,

Wang,

et al. 2024

Indoor Air

View full text Add to dashboard Cite

show abstract

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Chickens

Zhang

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

Rothia nasimurium is a facultative anaerobic Gram-positive coccus belonging to the Rothia genus of the Micrococcaceae family. While Rothia nasimurium is considered an opportunistic pathogen, to date few studies have investigated its pathogenicity and drug resistance. In January 2022, chickens at a poultry farm in China’s Xinjiang Uygur Autonomous Region became ill and died. Treatment with commonly used Chinese medicines and antibiotics was ineffective, causing economic losses to the poultry farm. In order to determine the cause of the disease in these poultry farm chickens, the isolation and identification of the pathogens in the livers and other internal organs of the sick and dead chickens were performed. Further, animal pathogenicity tests, antibiotic susceptibility tests, and the detection of antibiotic resistance genes were carried out to analyze the pathogenicity and drug resistance of the identified pathogens. A Gram-positive coccus was isolated from the livers of the diseased chickens. The isolate was resistant to 17 antibiotics, including ciprofloxacin, chloramphenicol, and florfenicol, and was only sensitive to penicillin, amikacin, and tigecycline, to varying degrees. The results of the drug resistance gene testing indicated that the isolated bacterium carried 13 kinds of resistance genes. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, morphological observations, biochemical tests, and 16S rRNA gene sequence analysis were performed on the isolated bacterium, and it was determined that the isolated bacterial strain was Rothia nasimurium. The animal pathogenicity tests showed that the isolate caused feather loss and death in chicks; the clinical symptoms and necropsy lesions of the test chicks were consistent with those observed in the farmed chickens. A review of the literature revealed that, to date, there are no reports of infection with Rothia nasimurium in chickens. Thus, in this study, Rothia nasimurium was isolated from chickens for the first time and an investigation of the biological characteristics of the bacterium was carried out in order to provide a reference for the clinical treatment, prevention, and control of Rothia nasimurium infection.

show abstract

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Cited by 2 publications

References 25 publications

Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Chickens

Contact Info

Product

Resources

About