Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: The use of cryostat sections

Chilosi, Marco; Pizzolo, Giovanni; Fiore-Donati, L; Bofill, Margarita; Jánossy, G.

doi:10.1038/bjc.1983.265

Cited by 59 publications

(17 citation statements)

References 40 publications

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“…10) are specific pointers of a cell type which is absent from the normal thymus (but see myasthenia gravis, Fig. 10) and bone marrow (B FALINI, DY MASON, personal communication;CHILOSI et al 1983).…”

Section: Epithelial and Accessory Cellsmentioning

confidence: 97%

Cellular Differentiation of Lymphoid Subpopulations and Their Microenvironments in the Human Thymus

Jánossy¹,

Bofill²,

Trejdosiewicz³

et al. 1986

The Human Thymus

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Section: Epithelial and Accessory Cellsmentioning

confidence: 97%

Cellular Differentiation of Lymphoid Subpopulations and Their Microenvironments in the Human Thymus

Jánossy¹,

Bofill²,

Trejdosiewicz³

et al. 1986

The Human Thymus

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“…Negative controls were incubated with isotype-matched irrelevant Abs. After 30 min incubation at 4ЊC, cells were washed twice with 2 ml of staining medium and flow cytometrically analysed with direct immunofluorescence and Immunohistochemical analysis of CD31 expression was performed on cryostat sections from snap-frozen portions of trephine biopsies using a previously described technique (Chilosi et al, 1983). CD31 Mab (clone JC/70A, Dako) was used at 1 : 20 dilution in PBS, and CD56 Mab (clone Leu19, Becton Dickinson) at 1 : 10 dilution.…”

Section: Methodsmentioning

confidence: 99%

Human myeloma cells express the CD38 ligand CD31

Vallario¹,

Chilosi²,

Adami³

et al. 1999

Br J Haematol

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Summary. Multiple myeloma (MM) plasma cells (PC) are CD38þ . A ligand for CD38 is the adhesion molecule CD31. By flow cytometry and immunocytochemistry we have investigated whether malignant PC co-express CD38 and CD31. All 68 patients studied were CD38þ . 14/14 monoclonal gammopathies of undetermined significance (MGUS) and 39/39 plasmacytic MM patients co-expressed CD38 and CD31 at high density. Only 1/11 plasmablastic MM and 1/4 plasma cell leukaemias (PCL) expressed CD31. These data indicated that PC malignancies co-expressed high levels of both CD38 and its ligand CD31, with the exception of plasmablastic MM and PCL.

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“…BM, spleen and lymph node samples were analyzed on cryostat and paraffin sections using techniques previously described in detail [14]. Monoclonal antibodies (MoAbs) used and their specificities are reported in table 1.…”

Section: Morphologic and Immunohistochemical Evaluationmentioning

confidence: 99%

A CD5+ Leukemic Lymphoma with Monocytoid Features: An Unusual B-Cell Lymphoma Mimicking Hairy-Cell Leukemia

Adami

Chilosi²,

Lestani³

et al. 1993

Acta Haematol

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A case of lymphoma presenting with features shared by hairy cell leukemia (HCL) and its variant, intermediate lymphocytic lymphoma (ILL) and monocytoid B-cell lymphoma (MBCL) is described. Clinical presentation and the morphological findings observed in peripheral blood and in bone marrow biopsy suggested an HCL; however, the tartrate-resistant acid phosphatase negativity of peripheral blood lymphocytes, the histologic pattern observed in the spleen, and the immunophenotyping of peripheral blood, spleen and bone marrow neoplastic lymphocytes (expression of CD5 and lack of CD25), were strongly against this hypothesis. The clinical course was aggressive. This report emphasizes the concept of the equivocal presentation of some ‘low-grade’ B-cell lymphomas. It is further pointed out that, even with complete and exhaustive morphological and immunological analyses, atypical cases may be encountered.

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Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: The use of cryostat sections

Cited by 59 publications

References 40 publications

Cellular Differentiation of Lymphoid Subpopulations and Their Microenvironments in the Human Thymus

Cellular Differentiation of Lymphoid Subpopulations and Their Microenvironments in the Human Thymus

Human myeloma cells express the CD38 ligand CD31

A CD5+ Leukemic Lymphoma with Monocytoid Features: An Unusual B-Cell Lymphoma Mimicking Hairy-Cell Leukemia

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