1996
DOI: 10.1111/j.1744-313x.1996.tb00137.x
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Routine Low and High Resolution Typing of the Hla‐drb Gene Using the Pcr‐mph (Microtitre Plate Hybridization) Method

Abstract: We describe HLA-DRB1 typing using polymerase chain reaction-based microtitre plate hybridization (PCR-MPH), which can process large numbers of samples. MPH typing is similar to an enzyme-linked immunosorbent assay (ELISA), in which a tandemly ligated sequence-specific oligonucleotide is immobilized on microtitre wells. The typing procedure consisted of two steps. In the first, PCR-MPH with 16 probes was performed to determine the specificities of the serological levels (DR1, DR2, DR3, DR4, DR11, DR12, DR13, DR… Show more

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Cited by 53 publications
(30 citation statements)
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“…DNA was extracted from peripheral white blood cells and typing was performed by PCR-MPH (microtitre plate hybridisation) (8).…”
Section: Hla Typingmentioning
confidence: 99%
“…DNA was extracted from peripheral white blood cells and typing was performed by PCR-MPH (microtitre plate hybridisation) (8).…”
Section: Hla Typingmentioning
confidence: 99%
“…We sequenced the TNXB gene in 48 healthy Japanese control subjects to search genetic variations further. Typing for the HLA-DRB1 locus was performed by the PCRmicrotiter plate hybridization (MPH) method using commercial typing kits (WAK Flow, Wakunaga, Hiroshima, Japan), as described previously (Kawai et al 1996).…”
Section: Dna Samples and Genotypingmentioning
confidence: 99%
“…DNA typing for identification of HLA class I and DRB1 alleles was performed by PCR sequence-specific primer methods and PCR microtiter plate hybridization methods (18), respectively. Type of HLA class I was determined using Micro SSP class I Generic Typing Kit (Veritas, Tokyo, Japan), and type of HLA-DRB1 was determined using a kit for HLA-DR typing (purchased from Wakunaga Pharmaceutical, Hirosima, Japan, and was licensed by Hoffmann-LaRoche, Nutley, NJ, and Roche Molecular Systems, Tutzing, Germany).…”
Section: Hla Typing Studiesmentioning
confidence: 99%