2019
DOI: 10.3390/molecules24244441
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Routine Management of Microalgae Using Autofluorescence from Chlorophyll

Abstract: From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial ap… Show more

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Cited by 43 publications
(28 citation statements)
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“…Chlorophyll is the intrinsic biomarker suitable to carefully monitor growing and quality of algae, which are very sensitive to changes in culture medium depending on the increase in their own growing biomass or on external factors. Devices based on cell counters detecting directly chlorophyll fluorescence together with additional parameters have been proposed to support and facilitate the industrial cultivation of microalgae [48,49]. This could help to adapt the production response to the increasing worldwide commercial interest in algae, because of their remarkable value as food and source of products valuable for dietary, nutraceutical and pharmacology purposes.…”
Section: Chlorophyll and Algaementioning
confidence: 99%
“…Chlorophyll is the intrinsic biomarker suitable to carefully monitor growing and quality of algae, which are very sensitive to changes in culture medium depending on the increase in their own growing biomass or on external factors. Devices based on cell counters detecting directly chlorophyll fluorescence together with additional parameters have been proposed to support and facilitate the industrial cultivation of microalgae [48,49]. This could help to adapt the production response to the increasing worldwide commercial interest in algae, because of their remarkable value as food and source of products valuable for dietary, nutraceutical and pharmacology purposes.…”
Section: Chlorophyll and Algaementioning
confidence: 99%
“…When illuminated with 480 nm-wavelength light, these smaller green vesicles emitted red light ( Fig. 5B ), similar to chlorophyll ( Takahashi 2019 . The larger orange vesicles emitted a green wavelength when excited at 480 nm.…”
Section: Resultsmentioning
confidence: 85%
“…Oleoresin extracts from H. pluvialis are known to emit yellow to red fluorescence when excited by light around 488 nm [ 21 ]. This fact can be exploited to measure phagocytosis of ASX-loaded microparticles by fluorescence microscopy and flow cytometry.…”
Section: Resultsmentioning
confidence: 99%